The Fluorescence Recovery After Photobleaching (FRAP) assay has been used to validate many of the SGC’s chemical probes against bromodomains. The published work can be found here "Frapid: achieving full automation of FRAP for chemical probe validation". Further reading: PMID:25097667
Frapid is a collection of scripts to automate a Zeiss LSM710 confocal for implementing FRAP as published in Biomedical Optics Xpress.
This website will provide updates and documentation for Frapid. Please access the links below to download the following files (rar files):
Frapid has been tested on a Windows PC installed with ZEN 2009 and MATLAB’s fuzzy logic and image analysis toolbox. Customising the Visual Basic script will require a USB dongle from Zeiss. Please contact Clarence Yapp (Clarence.yapp[at]ndm.ox.ac.uk) for queries.