Dalia’s PhD work in molecular cell biology and toxicology was carried out at the University of Manchester (UK). In her postdoctoral training at the University of Manchester and Ontario Cancer Institute, she investigated mechanisms of kinase signaling in stress response and c-Myc oncogene driven transcriptional programs associated with cellular stress and epigenetics. The scientific interests include transcription and chromatin driven cellular programs of pluripotency, differentiation, carcinogenesis and adaptation to the environment.
Current Toronto cell biology projects include functional studies and assays for the methyltransferases PRMT3, PRMT5, EZH2/1, SETD7, SETD8, WHSC1/NSD2/3 and, MLL family members, PRDM methyltransferases, methyl lysine binder WDR5 and chromatin binder UHRF1. The functional readouts for each target reflect the unique biology of that target. We address the activity of the chemical probes by looking at the enzymatic activity of the target in cells (immunofluorescence, immunoblotting, mass spectroscopy, ChIP), downstream effects on gene regulation, chromatin function and seek to advance the knowledge of the biological function of the target. We are particularly interested in the epigenetic states of cell pluripotency, differentiation and tumorigenicity.