Masoud Vedadi

Principal Investigator

University of Toronto
Group: 
Biophysics
+1 416 946 0897

Masoud is an Assistant Professor in the Department of Pharmacology and Toxicology at the University of Toronto. He received his Ph.D. from McGill University (1997) and worked as a postdoctoral fellow in the Department of Biochemistry in Concordia University, Canada, focusing on structural and functional studies of enzymes (1997-1999). Masoud then joined Affinium Pharmaceuticals where he worked as Associate Director of Protein Science. There, he and his team were involved in purifying, characterizing and crystallizing hundreds of proteins as well as developing novel methods of protein purification and characterization (1999-2003). He later joined the Structural Genomics Consortium in 2003 as head of the Molecular Biophysics group.

Research Areas

Molecular biophysics group of SGC Toronto is involved in various epigenetic related projects including characterization of histone methyltransferases (HMT). HMT family of proteins consist of enzymes that methylate lysine or arginine residues on histone tails as well as other proteins. Such modifications affect chromatin structure and play a significant regulatory role in gene expression. Many HMTs have been implicated in tumorigenesis and progression of multiple malignancies, and play essential roles in embryonic development and stem cell renewal. Overexpression of some HMTs has been observed and correlated positively with various types of cancers. I am interested in characterizing HMTs and discovering potent, selective and nontoxic inhibitors (chemical probes) of these proteins. The critical roles of HMTs in a variety of diseases suggest that many of these enzymes may be targets for a new generation of therapeutics. However, little is known about substrate specificity and kinetic behavior for many of the annotated HMTs. Thus, the field would benefit from such characterizations and availability of chemical probes.

Group Members

  • Abdellah Allali-Hassani
  • Guillermo Senisterra
  • Irene Chau
  • Taraneh Hajian
  • Fengling Li
  • Elisa Gibson
  • Mohammad Eram
  • Albina Bolotokova
  • Carlo C. dela Seña
  • Charles Leung, Postdoctoral Fellow
  • Slava Trush, Postdoctoral Fellow
  • Megha Abbey, Postdoctoral Fellow
Gain-of-function p53 mutants co-opt chromatin pathways to drive cancer growth.
Zhu J, Sammons MA, Donahue G, Dou Z, Vedadi M, Getlik M, Barsyte-Lovejoy D, Al-awar R, Katona BW, Shilatifard A, Huang J, Hua X, Arrowsmith CH, Berger SL
Nature. 2015 525(7568):206-11. doi: 10.1038/nature15251
PMID: 26331536

Pharmacological targeting of the Wdr5-MLL interaction in C/EBPα N-terminal leukemia.
Grebien F, Vedadi M, Getlik M, Giambruno R, Grover A, Avellino R, Skucha A, Vittori S, Kuznetsova E, Smil D, Barsyte-Lovejoy D, Li F, Poda G, Schapira M, Wu H, Dong A, Senisterra G, Stukalov A, Huber KV, Schönegger A, Marcellus R, Bilban M, Bock C, Brown PJ, Zuber J, Bennett KL, Al-Awar R, Delwel R, Nerlov C, Arrowsmith CH, Superti-Furga G
Nat. Chem. Biol.. 2015 . doi: 10.1038/nchembio.1859
PMID: 26167872

LLY-507, a Cell-Active, Potent and Selective Inhibitor of Protein Lysine Methyltransferase SMYD2.
Nguyen H, Allali-Hassani A, Antonysamy S, Chang S, Chen LH, Curtis C, Emtage S, Fan L, Gheyi T, Li F, Liu S, Martin JR, Mendel D, Olsen JB, Pelletier L, Shatseva T, Wu S, Zhang FF, Arrowsmith CH, Brown PJ, Campbell RM, Garcia BA, Barsyte-Lovejoy D, Mader M, Vedadi M
J. Biol. Chem.. 2015 . doi: 10.1074/jbc.M114.626861
PMID: 25825497

A Potent, Selective and Cell-Active Allosteric Inhibitor of Protein Arginine Methyltransferase 3 (PRMT3).
Kaniskan HÜ, Szewczyk MM, Yu Z, Eram MS, Yang X, Schmidt K, Luo X, Dai M, He F, Zang I, Lin Y, Kennedy S, Li F, Dobrovetsky E, Dong A, Smil D, Min SJ, Landon M, Lin-Jones J, Huang XP, Roth BL, Schapira M, Atadja P, Barsyte-Lovejoy D, Arrowsmith CH, Brown PJ, Zhao K, Jin J, Vedadi M
Angew. Chem. Int. Ed. Engl.. 2015 . doi: 10.1002/anie.201412154
PMID: 25728001

SMN and symmetric arginine dimethylation of RNA polymerase II C-terminal domain control termination.
Yanling Zhao D, Gish G, Braunschweig U, Li Y, Ni Z, Schmitges FW, Zhong G, Liu K, Li W, Moffat J, Vedadi M, Min J, Pawson TJ, Blencowe BJ, Greenblatt JF
Nature. 2015 . doi: 10.1038/nature16469
PMID: 26700805

(R)-PFI-2 is a potent and selective inhibitor of SETD7 methyltransferase activity in cells.
Barsyte-Lovejoy D, Li F, Oudhoff MJ, Tatlock JH, Dong A, Zeng H, Wu H, Freeman SA, Schapira M, Senisterra GA, Kuznetsova E, Marcellus R, Allali-Hassani A, Kennedy S, Lambert JP, Couzens AL, Aman A, Gingras AC, Al-Awar R, Fish PV, Gerstenberger BS, Roberts L, Benn CL, Grimley RL, Braam MJ, Rossi FM, Sudol M, Brown PJ, Bunnage ME, Owen DR, Zaph C, Vedadi M, Arrowsmith CH
Proc. Natl. Acad. Sci. U.S.A.. 2014 . doi: 10.1073/pnas.1407358111
PMID: 25136132

A Strategy for Modulation of Enzymes in the Ubiquitin System.
Ernst A, Avvakumov G, Tong J, Fan Y, Zhao Y, Alberts P, Persaud A, Walker JR, Neculai AM, Neculai D, Vorobyov A, Garg P, Beatty L, Chan PK, Juang YC, Landry MC, Yeh C, Zeqiraj E, Karamboulas K, Allali-Hassani A, Vedadi M, Tyers M, Moffat J, Sicheri F, Pelletier L, Durocher D, Raught B, Rotin D, Yang J, Moran MF, Dhe-Paganon S, Sidhu SS
Science. 2013 . doi: 10.1126/science.1230161
PMID: 23287719

An Orally Bioavailable Chemical Probe of the Lysine Methyltransferases EZH2 and EZH1.
Konze KD, Ma A, Li F, Barsyte-Lovejoy D, Parton T, Macnevin CJ, Liu F, Gao C, Huang XP, Kuznetsova E, Rougie M, Jiang A, Pattenden SG, Norris JL, James LI, Roth BL, Brown PJ, Frye SV, Arrowsmith CH, Hahn KM, Wang GG, Vedadi M, Jin J
ACS Chem. Biol.. 2013 . doi: 10.1021/cb400133j
PMID: 23614352

Small-molecule inhibition of MLL activity by disruption of its interaction with WDR5.
Senisterra G, Wu H, Allali-Hassani A, Wasney GA, Barsyte-Lovejoy D, Dombrovski L, Dong A, Nguyen KT, Smil D, Bolshan Y, Hajian T, He H, Seitova A, Chau I, Li F, Poda G, Couture JF, Brown PJ, Al-Awar R, Schapira M, Arrowsmith CH, Vedadi M
Biochem. J.. 2013 449(1):151-9. doi: 10.1042/BJ20121280
PMID: 22989411

An allosteric inhibitor of protein arginine methyltransferase 3.
Siarheyeva A, Senisterra G, Allali-Hassani A, Dong A, Dobrovetsky E, Wasney GA, Chau I, Marcellus R, Hajian T, Liu F, Korboukh I, Smil D, Bolshan Y, Min J, Wu H, Zeng H, Loppnau P, Poda G, Griffin C, Aman A, Brown PJ, Jin J, Al-Awar R, Arrowsmith CH, Schapira M, Vedadi M
Structure. 2012 20(8):1425-35. doi: 10.1016/j.str.2012.06.001
PMID: 22795084

A chemical probe selectively inhibits G9a and GLP methyltransferase activity in cells.
Vedadi M, Barsyte-Lovejoy D, Liu F, Rival-Gervier S, Allali-Hassani A, Labrie V, Wigle TJ, Dimaggio PA, Wasney GA, Siarheyeva A, Dong A, Tempel W, Wang SC, Chen X, Chau I, Mangano TJ, Huang XP, Simpson CD, Pattenden SG, Norris JL, Kireev DB, Tripathy A, Edwards A, Roth BL, Janzen WP, Garcia BA, Petronis A, Ellis J, Brown PJ, Frye SV, Arrowsmith CH, Jin J
Nat. Chem. Biol.. 2011 7(8):566-74. doi: 10.1038/nchembio.599
PMID: 21743462

2017

Discovery of Peptidomimetic Ligands of EED as Allosteric Inhibitors of PRC2.
Barnash KD, The J, Norris-Drouin JL, Cholensky SH, Worley BM, Li F, Stuckey JI, Brown PJ, Vedadi M, Arrowsmith CH, Frye SV, James LI
ACS Comb Sci. 06.02.2017 . doi: 10.1021/acscombsci.6b00174
PMID: 28165227

Targeting human SET1/MLL family of proteins.
Vedadi M, Blazer L, Eram MS, Barsyte-Lovejoy D, Arrowsmith CH, Hajian T
Protein Sci.. 04.02.2017 . doi: 10.1002/pro.3129
PMID: 28160335

The EED protein-protein interaction inhibitor A-395 inactivates the PRC2 complex.
He Y, Selvaraju S, Curtin ML, Jakob CG, Zhu H, Comess KM, Shaw B, The J, Lima-Fernandes E, Szewczyk MM, Cheng D, Klinge KL, Li HQ, Pliushchev M, Algire MA, Maag D, Guo J, Dietrich J, Panchal SC, Petros AM, Sweis RF, Torrent M, Bigelow LJ, Senisterra G, Li F, Kennedy S, Wu Q, Osterling DJ, Lindley DJ, Gao W, Galasinski S, Barsyte-Lovejoy D, Vedadi M, Buchanan FG, Arrowsmith CH, Chiang GG, Sun C, Pappano WN
Nat. Chem. Biol.. 30.01.2017 . doi: 10.1038/nchembio.2306
PMID: 28135237

Discovery of Potent and Selective Inhibitors for G9a-Like Protein (GLP) Lysine Methyltransferase.
Xiong Y, Li F, Babault N, Dong A, Zeng H, Wu H, Chen X, Arrowsmith CH, Brown PJ, Liu J, Vedadi M, Jin J
J. Med. Chem.. 30.01.2017 . doi: 10.1021/acs.jmedchem.6b01645
PMID: 28135087

The SUV4-20 inhibitor A-196 verifies a role for epigenetics in genomic integrity.
Bromberg KD, Mitchell TR, Upadhyay AK, Jakob CG, Jhala MA, Comess KM, Lasko LM, Li C, Tuzon CT, Dai Y, Li F, Eram MS, Nuber A, Soni NB, Manaves V, Algire MA, Sweis RF, Torrent M, Schotta G, Sun C, Michaelides MR, Shoemaker AR, Arrowsmith CH, Brown PJ, Santhakumar V, Martin A, Rice JC, Chiang GG, Vedadi M, Barsyte-Lovejoy D, Pappano WN
Nat. Chem. Biol.. 23.01.2017 . doi: 10.1038/nchembio.2282
PMID: 28114273

2016

Turning a Substrate Peptide into a Potent Inhibitor for the Histone Methyltransferase SETD8.
Judge RA, Zhu H, Upadhyay AK, Bodelle PM, Hutchins CW, Torrent M, Marin VL, Yu W, Vedadi M, Li F, Brown PJ, Pappano WN, Sun C, Petros AM
ACS Med Chem Lett. 08.12.2016 7(12):1102-1106. doi: 10.1021/acsmedchemlett.6b00303
PMID: 27994746

Structure-Based Design of a Covalent Inhibitor of the SET Domain-Containing Protein 8 (SETD8) Lysine Methyltransferase.
Butler KV, Ma A, Yu W, Li F, Tempel W, Babault N, Pittella-Silva F, Shao J, Wang J, Luo M, Vedadi M, Brown PJ, Arrowsmith CH, Jin J
J. Med. Chem.. 02.11.2016 . doi: 10.1021/acs.jmedchem.6b01244
PMID: 27804297

Discovery of Potent Pantothenamide Inhibitors of Staphylococcus aureus Pantothenate Kinase through a Minimal SAR Study: Inhibition Is Due to Trapping of the Product.
Hughes SJ, Barnard L, Mottaghi K, Tempel W, Antoshchenko T, Hong BS, Allali-Hassani A, Smil D, Vedadi M, Strauss E, Park HW
ACS Infect Dis. 09.09.2016 2(9):627-641. doi: 10.1021/acsinfecdis.6b00090
PMID: 27759386

Discovery of a Potent, Selective and Cell-active Dual Inhibitor of Protein Arginine Methyltransferase 4 and Protein Arginine Methyltransferase 6.
Shen Y, Szewczyk MM, Eram MS, Smil D, Kaniskan HÜ, de Freitas RF, Senisterra G, Li F, Schapira M, Brown PJ, Arrowsmith CH, Barsyte-Lovejoy D, Liu J, Vedadi M, Jin J
J. Med. Chem.. 01.09.2016 . doi: 10.1021/acs.jmedchem.6b01033
PMID: 27584694

A Suite of Biochemical Assays for Screening RNA Methyltransferase BCDIN3D.
Blazer LL, Li F, Kennedy S, Zheng YG, Arrowsmith CH, Vedadi M
J Biomol Screen. 31.08.2016 . doi: 10.1177/1087057116666276
PMID: 27581605

Structural basis of arginine asymmetrical dimethylation by PRMT6.
Wu H, Zheng W, Eram MS, Vhuiyan M, Dong A, Zeng H, He H, Brown P, Frankel A, Vedadi M, Luo M, Min J
Biochem. J.. 01.08.2016 . doi: 10.1042/BCJ20160537
PMID: 27480107

Structure-Activity Relationship Studies for Enhancer of Zeste Homologue 2 (EZH2) and Enhancer of Zeste Homologue 1 (EZH1) Inhibitors.
Yang X, Li F, Konze KD, Meslamani J, Ma A, Brown PJ, Zhou MM, Arrowsmith CH, Kaniskan HÜ, Vedadi M, Jin J
J. Med. Chem.. 28.07.2016 . doi: 10.1021/acs.jmedchem.6b00855
PMID: 27468126

New small molecule inhibitors of histone methyl transferase DOT1L with a nitrile as a non-traditional replacement for heavy halogen atoms.
Spurr SS, Bayle ED, Yu W, Li F, Tempel W, Vedadi M, Schapira M, Fish PV
Bioorg. Med. Chem. Lett.. 21.07.2016 . doi: 10.1016/j.bmcl.2016.07.041
PMID: 27485386

Discovery of a Potent and Selective Coactivator Associated Arginine Methyltransferase 1 (CARM1) Inhibitor by Virtual Screening.
Ferreira de Freitas R, Eram MS, Smil D, Szewczyk MM, Kennedy S, Brown PJ, Santhakumar V, Barsyte-Lovejoy D, Arrowsmith CH, Vedadi M, Schapira M
J. Med. Chem.. 08.07.2016 . doi: 10.1021/acs.jmedchem.6b00668
PMID: 27390919

Global Profiling of Acetyltransferase Feedback Regulation.
Montgomery DC, Garlick J, Kulkarni RA, Kennedy S, Allali-Hassani A, Kuo YM, Andrews AJ, Wu H, Vedadi M, Meier JL
J. Am. Chem. Soc.. 05.05.2016 . doi: 10.1021/jacs.6b03036
PMID: 27149119

PR Domain-Containing Protein 7 (PRDM7) is a Histone 3 Lysine 4 Trimethyltransferase.
Blazer LL, Lima-Fernandes E, Gibson E, Eram MS, Loppnau P, Arrowsmith CH, Schapira M, Vedadi M
J. Biol. Chem.. 29.04.2016 . doi: 10.1074/jbc.M116.721472
PMID: 27129774

Discovery and Characterization of a Highly Potent and Selective Aminopyrazoline-Based in vivo Probe (BAY-598) for the Protein Lysine Methyltransferase SMYD2.
Eggert E, Hillig RC, Köhr S, Stöckigt D, Weiske J, Barak N, Mowat J, Brumby T, Christ CD, Ter Laak A, Lang T, Fernández-Montalván AE, Badock V, Weinmann H, Hartung IV, Barsyte-Lovejoy D, Szewczyk M, Kennedy S, Li F, Vedadi M, Brown PJ, Santhakumar V, Arrowsmith CH, Stellfeld T, Stresemann C
J. Med. Chem.. 13.04.2016 . doi: 10.1021/acs.jmedchem.5b01890
PMID: 27075367

Structure-Based Optimization of a Small Molecule Antagonist of the Interaction Between WD Repeat-Containing Protein 5 (WDR5) and Mixed-Lineage Leukemia 1 (MLL1).
Getlik M, Smil D, Zepeda-Velázquez C, Bolshan Y, Poda G, Wu H, Dong A, Kuznetsova E, Marcellus R, Senisterra G, Dombrovski L, Hajian T, Kiyota T, Schapira M, Arrowsmith CH, Brown PJ, Vedadi M, Al-Awar R
J. Med. Chem.. 09.03.2016 . doi: 10.1021/acs.jmedchem.5b01630
PMID: 26958703

Discovery of a Potent Class I Protein Arginine Methyltransferase Fragment Inhibitor.
Ferreira de Freitas R, Eram MS, Szewczyk MM, Steuber H, Smil D, Wu H, Li F, Senisterra G, Dong A, Brown PJ, Hitchcock M, Moosmayer D, Stegmann CM, Egner U, Arrowsmith C, Barsyte-Lovejoy D, Vedadi M, Schapira M
J. Med. Chem.. 29.01.2016 . doi: 10.1021/acs.jmedchem.5b01772
PMID: 26824386

A cellular chemical probe targeting the chromodomains of Polycomb repressive complex 1.
Stuckey JI, Dickson BM, Cheng N, Liu Y, Norris JL, Cholensky SH, Tempel W, Qin S, Huber KG, Sagum C, Black K, Li F, Huang XP, Roth BL, Baughman BM, Senisterra G, Pattenden SG, Vedadi M, Brown PJ, Bedford MT, Min J, Arrowsmith CH, James LI, Frye SV
Nat. Chem. Biol.. 25.01.2016 . doi: 10.1038/nchembio.2007
PMID: 26807715

2015

A Radioactivity-Based Assay for Screening Human m6A-RNA Methyltransferase, METTL3-METTL14 Complex, and Demethylase ALKBH5.
Li F, Kennedy S, Hajian T, Gibson E, Seitova A, Xu C, Arrowsmith CH, Vedadi M
J Biomol Screen. 23.12.2015 . doi: 10.1177/1087057115623264
PMID: 26701100

SMN and symmetric arginine dimethylation of RNA polymerase II C-terminal domain control termination.
Yanling Zhao D, Gish G, Braunschweig U, Li Y, Ni Z, Schmitges FW, Zhong G, Liu K, Li W, Moffat J, Vedadi M, Min J, Pawson TJ, Blencowe BJ, Greenblatt JF
Nature. 23.12.2015 . doi: 10.1038/nature16469
PMID: 26700805

A Potent, Selective and Cell-active Inhibitor of Human Type I Protein Arginine Methyltransferases.
Eram MS, Shen Y, Szewczyk M, Wu H, Senisterra G, Li F, Butler KV, Kaniskan HÜ, Speed BA, Dela Seña C, Dong A, Zeng H, Schapira M, Brown PJ, Arrowsmith CH, Barsyte-Lovejoy D, Liu J, Vedadi M, Jin J
ACS Chem. Biol.. 24.11.2015 . doi: 10.1021/acschembio.5b00839
PMID: 26598975

Design of a fluorescent ligand targeting the S-adenosylmethionine binding site of the histone methyltransferase MLL1.
Luan Y, Blazer LL, Hu H, Hajian T, Zhang J, Wu H, Houliston S, Arrowsmith CH, Vedadi M, Zheng YG
Org. Biomol. Chem.. 06.11.2015 . doi: 10.1039/c5ob01794g
PMID: 26541578

WDR5 supports an N-Myc transcriptional complex that drives a pro-tumorigenic gene expression signature in neuroblastoma.
Sun Y, Bell JL, Carter DR, Gherardi S, Poulos RC, Milazzo G, Wong JW, Al-Awar R, Tee AE, Liu PY, Liu B, Atmadibrata B, Wong M, Trahair T, Zhao Q, Shohet JM, Haupt Y, Schulte JH, Brown PJ, Arrowsmith CH, Vedadi M, MacKenzie KL, Huttelmaier S, Perini G, Marshall GM, Braithwaite A, Liu T
Cancer Res.. 15.10.2015 . doi: 10.1158/0008-5472.CAN-15-0423
PMID: 26471359

Gain-of-function p53 mutants co-opt chromatin pathways to drive cancer growth.
Zhu J, Sammons MA, Donahue G, Dou Z, Vedadi M, Getlik M, Barsyte-Lovejoy D, Al-awar R, Katona BW, Shilatifard A, Huang J, Hua X, Arrowsmith CH, Berger SL
Nature. 10.09.2015 525(7568):206-11. doi: 10.1038/nature15251
PMID: 26331536

Dual EZH2 and EHMT2 histone methyltransferase inhibition increases biological efficacy in breast cancer cells.
Curry E, Green I, Chapman-Rothe N, Shamsaei E, Kandil S, Cherblanc FL, Payne L, Bell E, Ganesh T, Srimongkolpithak N, Caron J, Li F, Uren AG, Snyder JP, Vedadi M, Fuchter MJ, Brown R
Clin Epigenetics. 21.08.2015 7(1):84. doi: 10.1186/s13148-015-0118-9
PMID: 26300989

Pharmacological targeting of the Wdr5-MLL interaction in C/EBPα N-terminal leukemia.
Grebien F, Vedadi M, Getlik M, Giambruno R, Grover A, Avellino R, Skucha A, Vittori S, Kuznetsova E, Smil D, Barsyte-Lovejoy D, Li F, Poda G, Schapira M, Wu H, Dong A, Senisterra G, Stukalov A, Huber KV, Schönegger A, Marcellus R, Bilban M, Bock C, Brown PJ, Zuber J, Bennett KL, Al-Awar R, Delwel R, Nerlov C, Arrowsmith CH, Superti-Furga G
Nat. Chem. Biol.. 13.07.2015 . doi: 10.1038/nchembio.1859
PMID: 26167872

Discovery of A-893, A New Cell-Active Benzoxazinone Inhibitor of Lysine Methyltransferase SMYD2.
Sweis RF, Wang Z, Algire M, Arrowsmith CH, Brown PJ, Chiang GG, Guo J, Jakob CG, Kennedy S, Li F, Maag D, Shaw B, Soni NB, Vedadi M, Pappano WN
ACS Med Chem Lett. 11.06.2015 6(6):695-700. doi: 10.1021/acsmedchemlett.5b00124
PMID: 26101576

Kinetic characterization of human histone H3 lysine 36 methyltransferases, ASH1L and SETD2.
Eram MS, Kuznetsova E, Li F, Lima-Fernandes E, Kennedy S, Chau I, Arrowsmith CH, Schapira M, Vedadi M
Biochim. Biophys. Acta. 19.05.2015 . doi: 10.1016/j.bbagen.2015.05.013
PMID: 26002201

Discovery of a Dual PRMT5-PRMT7 Inhibitor.
Smil D, Eram MS, Li F, Kennedy S, Szewczyk MM, Brown PJ, Barsyte-Lovejoy D, Arrowsmith CH, Vedadi M, Schapira M
ACS Med Chem Lett. 09.04.2015 6(4):408-12. doi: 10.1021/ml500467h
PMID: 25893041

LLY-507, a Cell-Active, Potent and Selective Inhibitor of Protein Lysine Methyltransferase SMYD2.
Nguyen H, Allali-Hassani A, Antonysamy S, Chang S, Chen LH, Curtis C, Emtage S, Fan L, Gheyi T, Li F, Liu S, Martin JR, Mendel D, Olsen JB, Pelletier L, Shatseva T, Wu S, Zhang FF, Arrowsmith CH, Brown PJ, Campbell RM, Garcia BA, Barsyte-Lovejoy D, Mader M, Vedadi M
J. Biol. Chem.. 30.03.2015 . doi: 10.1074/jbc.M114.626861
PMID: 25825497

A Potent, Selective and Cell-Active Allosteric Inhibitor of Protein Arginine Methyltransferase 3 (PRMT3).
Kaniskan HÜ, Szewczyk MM, Yu Z, Eram MS, Yang X, Schmidt K, Luo X, Dai M, He F, Zang I, Lin Y, Kennedy S, Li F, Dobrovetsky E, Dong A, Smil D, Min SJ, Landon M, Lin-Jones J, Huang XP, Roth BL, Schapira M, Atadja P, Barsyte-Lovejoy D, Arrowsmith CH, Brown PJ, Zhao K, Jin J, Vedadi M
Angew. Chem. Int. Ed. Engl.. 27.02.2015 . doi: 10.1002/anie.201412154
PMID: 25728001

2014

Selective inhibition of EZH2 and EZH1 enzymatic activity by a small molecule suppresses MLL-rearranged leukemia.
Xu B, On DM, Ma A, Parton T, Konze KD, Pattenden SG, Allison DF, Cai L, Rockowitz S, Liu S, Liu Y, Li F, Vedadi M, Frye SV, Garcia BA, Zheng D, Jin J, Wang GG
Blood. 13.11.2014 . doi: 10.1182/blood-2014-06-581082
PMID: 25395428

(R)-PFI-2 is a potent and selective inhibitor of SETD7 methyltransferase activity in cells.
Barsyte-Lovejoy D, Li F, Oudhoff MJ, Tatlock JH, Dong A, Zeng H, Wu H, Freeman SA, Schapira M, Senisterra GA, Kuznetsova E, Marcellus R, Allali-Hassani A, Kennedy S, Lambert JP, Couzens AL, Aman A, Gingras AC, Al-Awar R, Fish PV, Gerstenberger BS, Roberts L, Benn CL, Grimley RL, Braam MJ, Rossi FM, Sudol M, Brown PJ, Bunnage ME, Owen DR, Zaph C, Vedadi M, Arrowsmith CH
Proc. Natl. Acad. Sci. U.S.A.. 18.08.2014 . doi: 10.1073/pnas.1407358111
PMID: 25136132

Discovery of a Selective, Substrate-competitive Inhibitor of the Lysine Methyltransferase SETD8.
Ma A, Yu W, Li F, Bleich RM, Herold JM, Butler KV, Norris JL, Korboukh V, Tripathy A, Janzen WP, Arrowsmith CH, Frye SV, Vedadi M, Brown PJ, Jin J
J. Med. Chem.. 17.07.2014 . doi: 10.1021/jm500871s
PMID: 25032507

A scintillation proximity assay for histone demethylases.
Yu W, Eram MS, Hajian T, Szykowska A, Burgess-Brown N, Vedadi M, Brown PJ
Anal. Biochem.. 08.07.2014 . doi: 10.1016/j.ab.2014.06.023
PMID: 25010373

Structural basis for histone mimicry and hijacking of host proteins by influenza virus protein NS1.
Qin S, Liu Y, Tempel W, Eram MS, Bian C, Liu K, Senisterra G, Crombet L, Vedadi M, Min J
Nat Commun. 24.05.2014 5:3952. doi: 10.1038/ncomms4952
PMID: 24853335

Trimethylation of Histone H3 Lysine 36 by Human Methyltransferase PRDM9.
Eram MS, Bustos SP, Lima-Fernandes E, Siarheyeva A, Senisterra G, Hajian T, Chau I, Duan S, Wu H, Dombrovski L, Schapira M, Arrowsmith CH, Vedadi M
J. Biol. Chem.. 14.03.2014 . doi: 10.1074/jbc.M113.523183
PMID: 24634223

A Basic Post-SET Extension of NSDs Is Essential for Nucleosome Binding In Vitro.
Allali-Hassani A, Kuznetsova E, Hajian T, Wu H, Dombrovski L, Li Y, Gräslund S, Arrowsmith CH, Schapira M, Vedadi M
J Biomol Screen. 04.03.2014 . doi: 10.1177/1087057114525854
PMID: 24595546

Discovery and development of potent and selective inhibitors of histone methyltransferase g9a.
Sweis RF, Pliushchev M, Brown PJ, Guo J, Li F, Maag D, Petros AM, Soni NB, Tse C, Vedadi M, Michaelides MR, Chiang GG, Pappano WN
ACS Med Chem Lett. 13.02.2014 5(2):205-9. doi: 10.1021/ml400496h
PMID: 24900801

A Chemical Tool for In Vitro and In Vivo Precipitation of Lysine Methyltransferase G9a.
Konze KD, Pattenden SG, Liu F, Barsyte-Lovejoy D, Li F, Simon JM, Davis IJ, Vedadi M, Jin J
ChemMedChem. 17.01.2014 . doi: 10.1002/cmdc.201300450
PMID: 24443078

Discovery and Development of Potent and Selective Inhibitors of Histone Methyltransferase G9a
Sweis RF, Pliushchev M, Brown PJ, Guo J, Li F, Maag D, Petros AM, Soni NB, Tse C, Vedadi M, Michaelides MR, Chiang GG, PappanoWN
ACS Med. Chem. Lett. 04.01.2014 . doi:
PMID:

2013

Structure of the Catalytic Domain of EZH2 Reveals Conformational Plasticity in Cofactor and Substrate Binding Sites and Explains Oncogenic Mutations.
Wu H, Zeng H, Dong A, Li F, He H, Senisterra G, Seitova A, Duan S, Brown PJ, Vedadi M, Arrowsmith CH, Schapira M
PLoS ONE. 19.12.2013 8(12):e83737. doi: 10.1371/journal.pone.0083737
PMID: 24367611

Crystal structures of the human histone H4K20 methyltransferases SUV420H1 and SUV420H2.
Wu H, Siarheyeva A, Zeng H, Lam R, Dong A, Wu XH, Li Y, Schapira M, Vedadi M, Min J
FEBS Lett.. 29.11.2013 587(23):3859-68. doi: 10.1016/j.febslet.2013.10.020
PMID: 24396869

Discovery of an in vivo Chemical Probe of the Lysine Methyltransferases G9a and GLP.
Liu F, Barsyte-Lovejoy D, Li F, Xiong Y, Korboukh VK, Huang X, Allali-Hassani A, Janzen WP, Roth BL, Frye SV, Arrowsmith CH, Brown PJ, Vedadi M, Jin J
J. Med. Chem.. 08.10.2013 . doi: 10.1021/jm401480r
PMID: 24102134

Control of the Hippo Pathway by Set7-Dependent Methylation of Yap.
Oudhoff MJ, Freeman SA, Couzens AL, Antignano F, Kuznetsova E, Min PH, Northrop JP, Lehnertz B, Barsyte-Lovejoy D, Vedadi M, Arrowsmith CH, Nishina H, Gold MR, Rossi FM, Gingras AC, Zaph C
Dev. Cell. 09.07.2013 . doi: 10.1016/j.devcel.2013.05.025
PMID: 23850191

An Orally Bioavailable Chemical Probe of the Lysine Methyltransferases EZH2 and EZH1.
Konze KD, Ma A, Li F, Barsyte-Lovejoy D, Parton T, Macnevin CJ, Liu F, Gao C, Huang XP, Kuznetsova E, Rougie M, Jiang A, Pattenden SG, Norris JL, James LI, Roth BL, Brown PJ, Frye SV, Arrowsmith CH, Hahn KM, Wang GG, Vedadi M, Jin J
ACS Chem. Biol.. 24.04.2013 . doi: 10.1021/cb400133j
PMID: 23614352

Bromo-deaza-SAH: A potent and selective DOT1L inhibitor.
Yu W, Smil D, Li F, Tempel W, Fedorov O, Nguyen KT, Bolshan Y, Al-Awar R, Knapp S, Arrowsmith CH, Vedadi M, Brown PJ, Schapira M
Bioorg. Med. Chem.. 01.04.2013 21(7):1787-94. doi: 10.1016/j.bmc.2013.01.049
PMID: 23433670

Exploiting an Allosteric Binding Site of PRMT3 Yields Potent and Selective Inhibitors.
Liu F, Li F, Ma A, Dobrovetsky E, Dong A, Gao C, Korboukh I, Liu J, Smil D, Brown PJ, Frye SV, Arrowsmith CH, Schapira M, Vedadi M, Jin J
J. Med. Chem.. 14.03.2013 56(5):2110-24. doi: 10.1021/jm3018332
PMID: 23445220

Synthesis, Optimization, and Evaluation of Novel Small Molecules as Antagonists of WDR5-MLL Interaction.
Bolshan Y, Getlik M, Kuznetsova E, Wasney GA, Hajian T, Poda G, Nguyen KT, Wu H, Dombrovski L, Dong A, Senisterra G, Schapira M, Arrowsmith CH, Brown PJ, Al-Awar R, Vedadi M, Smil D
ACS Med Chem Lett. 14.03.2013 4(3):353-7. doi: 10.1021/ml300467n
PMID: 24900672

Strategy to Target the Substrate Binding site of SET Domain Protein Methyltransferases.
Nguyen KT, Li F, Poda G, Smil D, Vedadi M, Schapira M
J Chem Inf Model. 01.03.2013 . doi: 10.1021/ci300596x
PMID: 23410263

A Strategy for Modulation of Enzymes in the Ubiquitin System.
Ernst A, Avvakumov G, Tong J, Fan Y, Zhao Y, Alberts P, Persaud A, Walker JR, Neculai AM, Neculai D, Vorobyov A, Garg P, Beatty L, Chan PK, Juang YC, Landry MC, Yeh C, Zeqiraj E, Karamboulas K, Allali-Hassani A, Vedadi M, Tyers M, Moffat J, Sicheri F, Pelletier L, Durocher D, Raught B, Rotin D, Yang J, Moran MF, Dhe-Paganon S, Sidhu SS
Science. 03.01.2013 . doi: 10.1126/science.1230161
PMID: 23287719

Small-molecule inhibition of MLL activity by disruption of its interaction with WDR5.
Senisterra G, Wu H, Allali-Hassani A, Wasney GA, Barsyte-Lovejoy D, Dombrovski L, Dong A, Nguyen KT, Smil D, Bolshan Y, Hajian T, He H, Seitova A, Chau I, Li F, Poda G, Couture JF, Brown PJ, Al-Awar R, Schapira M, Arrowsmith CH, Vedadi M
Biochem. J.. 01.01.2013 449(1):151-9. doi: 10.1042/BJ20121280
PMID: 22989411

2012

Nahuoic Acid A Produced by a Streptomyces sp. Isolated From a Marine Sediment Is a Selective SAM-Competitive Inhibitor of the Histone Methyltransferase SETD8.
Williams DE, Dalisay DS, Li F, Amphlett J, Maneerat W, Chavez MA, Wang YA, Matainaho T, Yu W, Brown PJ, Arrowsmith CH, Vedadi M, Andersen RJ
Org. Lett.. 28.12.2012 . doi: 10.1021/ol303416k
PMID: 23272941

Catalytic site remodelling of the DOT1L methyltransferase by selective inhibitors.
Yu W, Chory EJ, Wernimont AK, Tempel W, Scopton A, Federation A, Marineau JJ, Qi J, Barsyte-Lovejoy D, Yi J, Marcellus R, Iacob RE, Engen JR, Griffin C, Aman A, Wienholds E, Li F, Pineda J, Estiu G, Shatseva T, Hajian T, Al-Awar R, Dick JE, Vedadi M, Brown PJ, Arrowsmith CH, Bradner JE, Schapira M
Nat Commun. 18.12.2012 3:1288. doi: 10.1038/ncomms2304
PMID: 23250418

Sinefungin derivatives as inhibitors and structure probes of protein lysine methyltransferase SETD2.
Zheng W, Ibáñez G, Wu H, Blum G, Zeng H, Dong A, Li F, Hajian T, Allali-Hassani A, Amaya MF, Siarheyeva A, Yu W, Brown PJ, Schapira M, Vedadi M, Min J, Luo M
J. Am. Chem. Soc.. 31.10.2012 134(43):18004-14. doi: 10.1021/ja307060p
PMID: 23043551

An allosteric inhibitor of protein arginine methyltransferase 3.
Siarheyeva A, Senisterra G, Allali-Hassani A, Dong A, Dobrovetsky E, Wasney GA, Chau I, Marcellus R, Hajian T, Liu F, Korboukh I, Smil D, Bolshan Y, Min J, Wu H, Zeng H, Loppnau P, Poda G, Griffin C, Aman A, Brown PJ, Jin J, Al-Awar R, Arrowsmith CH, Schapira M, Vedadi M
Structure. 08.08.2012 20(8):1425-35. doi: 10.1016/j.str.2012.06.001
PMID: 22795084

Sequence-specific recognition of a PxLPxI/L motif by an ankyrin repeat tumbler lock.
Xu C, Jin J, Bian C, Lam R, Tian R, Weist R, You L, Nie J, Bochkarev A, Tempel W, Tan CS, Wasney GA, Vedadi M, Gish GD, Arrowsmith CH, Pawson T, Yang XJ, Min J
Sci Signal. 29.05.2012 5(226):ra39. doi: 10.1126/scisignal.2002979
PMID: 22649097

2011

Thermal denaturation assays in chemical biology.
Senisterra G, Chau I, Vedadi M
Assay Drug Dev Technol. 08.11.2011 10(2):128-36. doi: 10.1089/adt.2011.0390
PMID: 22066913

Fluorescence-based methods for screening writers and readers of histone methyl marks.
Allali-Hassani A, Wasney GA, Siarheyeva A, Hajian T, Arrowsmith CH, Vedadi M
J Biomol Screen. 04.10.2011 17(1):71-84. doi: 10.1177/1087057111422256
PMID: 21972038

The Cryptosporidium parvum kinome.
Artz JD, Wernimont AK, Allali-Hassani A, Zhao Y, Amani M, Lin YH, Senisterra G, Wasney GA, Fedorov O, King O, Roos A, Lunin VV, Qiu W, Finerty P, Hutchinson A, Chau I, von Delft F, MacKenzie F, Lew J, Kozieradzki I, Vedadi M, Schapira M, Zhang C, Shokat K, Heightman T, Hui R
BMC Genomics. 30.09.2011 12:478. doi: 10.1186/1471-2164-12-478
PMID: 21962082

Optimization of cellular activity of G9a inhibitors 7-aminoalkoxy-quinazolines.
Liu F, Barsyte-Lovejoy D, Allali-Hassani A, He Y, Herold JM, Chen X, Yates CM, Frye SV, Brown PJ, Huang J, Vedadi M, Arrowsmith CH, Jin J
J. Med. Chem.. 08.09.2011 54(17):6139-50. doi: 10.1021/jm200903z
PMID: 21780790

Characterization of 14-3-3 proteins from Cryptosporidium parvum.
Brokx SJ, Wernimont AK, Dong A, Wasney GA, Lin YH, Lew J, Vedadi M, Lee WH, Hui R
PLoS ONE. 11.08.2011 6(8):e14827. doi: 10.1371/journal.pone.0014827
PMID: 21853016

A chemical probe selectively inhibits G9a and GLP methyltransferase activity in cells.
Vedadi M, Barsyte-Lovejoy D, Liu F, Rival-Gervier S, Allali-Hassani A, Labrie V, Wigle TJ, Dimaggio PA, Wasney GA, Siarheyeva A, Dong A, Tempel W, Wang SC, Chen X, Chau I, Mangano TJ, Huang XP, Simpson CD, Pattenden SG, Norris JL, Kireev DB, Tripathy A, Edwards A, Roth BL, Janzen WP, Garcia BA, Petronis A, Ellis J, Brown PJ, Frye SV, Arrowsmith CH, Jin J
Nat. Chem. Biol.. 10.07.2011 7(8):566-74. doi: 10.1038/nchembio.599
PMID: 21743462

An allosteric inhibitor of the human Cdc34 ubiquitin-conjugating enzyme.
Ceccarelli DF, Tang X, Pelletier B, Orlicky S, Xie W, Plantevin V, Neculai D, Chou YC, Ogunjimi A, Al-Hakim A, Varelas X, Koszela J, Wasney GA, Vedadi M, Dhe-Paganon S, Cox S, Xu S, Lopez-Girona A, Mercurio F, Wrana J, Durocher D, Meloche S, Webb DR, Tyers M, Sicheri F
Cell. 24.06.2011 145(7):1075-87. doi: 10.1016/j.cell.2011.05.039
PMID: 21683433

Small-molecule ligands of methyl-lysine binding proteins.
Herold JM, Wigle TJ, Norris JL, Lam R, Korboukh VK, Gao C, Ingerman LA, Kireev DB, Senisterra G, Vedadi M, Tripathy A, Brown PJ, Arrowsmith CH, Jin J, Janzen WP, Frye SV
J. Med. Chem.. 14.04.2011 54(7):2504-11. doi: 10.1021/jm200045v
PMID: 21417280

Recognition and specificity determinants of the human cbx chromodomains.
Kaustov L, Ouyang H, Amaya M, Lemak A, Nady N, Duan S, Wasney GA, Li Z, Vedadi M, Schapira M, Min J, Arrowsmith CH
J. Biol. Chem.. 07.01.2011 286(1):521-9. doi: 10.1074/jbc.M110.191411
PMID: 21047797

2010

Structural studies of the tandem Tudor domains of fragile X mental retardation related proteins FXR1 and FXR2.
Adams-Cioaba MA, Guo Y, Bian C, Amaya MF, Lam R, Wasney GA, Vedadi M, Xu C, Min J
PLoS ONE. 02.11.2010 5(11):e13559. doi: 10.1371/journal.pone.0013559
PMID: 21072162

Protein lysine methyltransferase G9a inhibitors: design, synthesis, and structure activity relationships of 2,4-diamino-7-aminoalkoxy-quinazolines.
Liu F, Chen X, Allali-Hassani A, Quinn AM, Wigle TJ, Wasney GA, Dong A, Senisterra G, Chau I, Siarheyeva A, Norris JL, Kireev DB, Jadhav A, Herold JM, Janzen WP, Arrowsmith CH, Frye SV, Brown PJ, Simeonov A, Vedadi M, Jin J
J. Med. Chem.. 12.08.2010 53(15):5844-57. doi: 10.1021/jm100478y
PMID: 20614940

Crystal structures of human choline kinase isoforms in complex with hemicholinium-3: single amino acid near the active site influences inhibitor sensitivity.
Hong BS, Allali-Hassani A, Tempel W, Finerty PJ, Mackenzie F, Dimov S, Vedadi M, Park HW
J. Biol. Chem.. 21.05.2010 285(21):16330-40. doi: 10.1074/jbc.M109.039024
PMID: 20299452

Biophysical characterization of recombinant proteins: a key to higher structural genomics success.
Vedadi M, Arrowsmith CH, Allali-Hassani A, Senisterra G, Wasney GA
J. Struct. Biol.. 11.05.2010 172(1):107-19. doi: 10.1016/j.jsb.2010.05.005
PMID: 20466062

A chemiluminescence-based method for identification of histone lysine methyltransferase inhibitors.
Quinn AM, Allali-Hassani A, Vedadi M, Simeonov A
Mol Biosyst. 10.05.2010 6(5):782-8. doi: 10.1039/b921912a
PMID: 20567762

Structures of apicomplexan calcium-dependent protein kinases reveal mechanism of activation by calcium.
Wernimont AK, Artz JD, Finerty P, Lin YH, Amani M, Allali-Hassani A, Senisterra G, Vedadi M, Tempel W, Mackenzie F, Chau I, Lourido S, Sibley LD, Hui R
Nat. Struct. Mol. Biol.. 02.05.2010 17(5):596-601. doi: 10.1038/nsmb.1795
PMID: 20436473

Assessing the stability of membrane proteins to detect ligand binding using differential static light scattering.
Senisterra GA, Ghanei H, Khutoreskaya G, Dobrovetsky E, Edwards AM, Privé GG, Vedadi M
J Biomol Screen. 11.02.2010 15(3):314-20. doi: 10.1177/1087057109357117
PMID: 20150591

Structural biology of human H3K9 methyltransferases.
Wu H, Min J, Lunin VV, Antoshenko T, Dombrovski L, Zeng H, Allali-Hassani A, Campagna-Slater V, Vedadi M, Arrowsmith CH, Plotnikov AN, Schapira M
PLoS ONE. 11.01.2010 5(1):e8570. doi: 10.1371/journal.pone.0008570
PMID: 20084102

2009

Discovery of a 2,4-diamino-7-aminoalkoxyquinazoline as a potent and selective inhibitor of histone lysine methyltransferase G9a.
Liu F, Chen X, Allali-Hassani A, Quinn AM, Wasney GA, Dong A, Barsyte D, Kozieradzki I, Senisterra G, Chau I, Siarheyeva A, Kireev DB, Jadhav A, Herold JM, Frye SV, Arrowsmith CH, Brown PJ, Simeonov A, Vedadi M, Jin J
J. Med. Chem.. 24.12.2009 52(24):7950-3. doi: 10.1021/jm901543m
PMID: 19891491

Screening for inhibitors of low-affinity epigenetic peptide-protein interactions: an AlphaScreen-based assay for antagonists of methyl-lysine binding proteins.
Wigle TJ, Herold JM, Senisterra GA, Vedadi M, Kireev DB, Arrowsmith CH, Frye SV, Janzen WP
J Biomol Screen. 11.12.2009 15(1):62-71. doi: 10.1177/1087057109352902
PMID: 20008125

A survey of proteins encoded by non-synonymous single nucleotide polymorphisms reveals a significant fraction with altered stability and activity.
Allali-Hassani A, Wasney GA, Chau I, Hong BS, Senisterra G, Loppnau P, Shi Z, Moult J, Edwards AM, Arrowsmith CH, Park HW, Schapira M, Vedadi M
Biochem. J.. 15.11.2009 424(1):15-26. doi: 10.1042/BJ20090723
PMID: 19702579

Structural studies of a four-MBT repeat protein MBTD1.
Eryilmaz J, Pan P, Amaya MF, Allali-Hassani A, Dong A, Adams-Cioaba MA, Mackenzie F, Vedadi M, Min J
PLoS ONE. 20.10.2009 4(10):e7274. doi: 10.1371/journal.pone.0007274
PMID: 19841675

Specificity of Campylobacter jejuni adhesin PEB3 for phosphates and structural differences among its ligand complexes.
Min T, Vedadi M, Watson DC, Wasney GA, Munger C, Cygler M, Matte A, Young NM
Biochemistry. 14.04.2009 48(14):3057-67. doi: 10.1021/bi802195d
PMID: 19236052

Methylation-state-specific recognition of histones by the MBT repeat protein L3MBTL2.
Guo Y, Nady N, Qi C, Allali-Hassani A, Zhu H, Pan P, Adams-Cioaba MA, Amaya MF, Dong A, Vedadi M, Schapira M, Read RJ, Arrowsmith CH, Min J
Nucleic Acids Res.. 20.02.2009 37(7):2204-10. doi: 10.1093/nar/gkp086
PMID: 19233876

2008

Structural insights into the inhibited states of the Mer receptor tyrosine kinase.
Huang X, Finerty P, Walker JR, Butler-Cole C, Vedadi M, Schapira M, Parker SA, Turk BE, Thompson DA, Dhe-Paganon S
J. Struct. Biol.. 05.11.2008 165(2):88-96. doi: 10.1016/j.jsb.2008.10.003
PMID: 19028587

Application of high-throughput isothermal denaturation to assess protein stability and screen for ligands.
Senisterra GA, Soo Hong B, Park HW, Vedadi M
J Biomol Screen. 30.04.2008 13(5):337-42. doi: 10.1177/1087057108317825
PMID: 18448703

Human HDAC7 harbors a class IIa histone deacetylase-specific zinc binding motif and cryptic deacetylase activity.
Schuetz A, Min J, Allali-Hassani A, Schapira M, Shuen M, Loppnau P, Mazitschek R, Kwiatkowski NP, Lewis TA, Maglathin RL, McLean TH, Bochkarev A, Plotnikov AN, Vedadi M, Arrowsmith CH
J. Biol. Chem.. 25.04.2008 283(17):11355-63. doi: 10.1074/jbc.M707362200
PMID: 18285338

2007

L3MBTL1 recognition of mono- and dimethylated histones.
Min J, Allali-Hassani A, Nady N, Qi C, Ouyang H, Liu Y, MacKenzie F, Vedadi M, Arrowsmith CH
Nat. Struct. Mol. Biol.. 18.11.2007 14(12):1229-30. doi: 10.1038/nsmb1340
PMID: 18026117

In situ proteolysis for protein crystallization and structure determination.
Dong A, Xu X, Edwards AM, Chang C, Chruszcz M, Cuff M, Cymborowski M, Di Leo R, Egorova O, Evdokimova E, Filippova E, Gu J, Guthrie J, Ignatchenko A, Joachimiak A, Klostermann N, Kim Y, Korniyenko Y, Minor W, Que Q, Savchenko A, Skarina T, Tan K, Yakunin A, Yee A, Yim V, Zhang R, Zheng H, Akutsu M, Arrowsmith C, Avvakumov GV, Bochkarev A, Dahlgren LG, Dhe-Paganon S, Dimov S, Dombrovski L, Finerty P, Flodin S, Flores A, Gräslund S, Hammerström M, Herman MD, Hong BS, Hui R, Johansson I, Liu Y, Nilsson M, Nedyalkova L, Nordlund P, Nyman T, Min J, Ouyang H, Park HW, Qi C, Rabeh W, Shen L, Shen Y, Sukumard D, Tempel W, Tong Y, Tresagues L, Vedadi M, Walker JR, Weigelt J, Welin M, Wu H, Xiao T, Zeng H, Zhu H
Nat. Methods. 04.11.2007 4(12):1019-21. doi: 10.1038/nmeth1118
PMID: 17982461

Crystal structures of human pantothenate kinases. Insights into allosteric regulation and mutations linked to a neurodegeneration disorder.
Hong BS, Senisterra G, Rabeh WM, Vedadi M, Leonardi R, Zhang YM, Rock CO, Jackowski S, Park HW
J. Biol. Chem.. 21.09.2007 282(38):27984-93. doi: 10.1074/jbc.M701915200
PMID: 17631502

Glyoxylate and pyruvate are antagonistic effectors of the Escherichia coli IclR transcriptional regulator.
Lorca GL, Ezersky A, Lunin VV, Walker JR, Altamentova S, Evdokimova E, Vedadi M, Bochkarev A, Savchenko A
J. Biol. Chem.. 01.06.2007 282(22):16476-91. doi: 10.1074/jbc.M610838200
PMID: 17426033

Structural and chemical profiling of the human cytosolic sulfotransferases.
Allali-Hassani A, Pan PW, Dombrovski L, Najmanovich R, Tempel W, Dong A, Loppnau P, Martin F, Thornton J, Thonton J, Edwards AM, Bochkarev A, Plotnikov AN, Vedadi M, Arrowsmith CH
PLoS Biol.. 12.04.2007 5(5):e97. doi: 10.1371/journal.pbio.0050097
PMID: 17425406

Structural basis of allele variation of human thiopurine-S-methyltransferase.
Wu H, Horton JR, Battaile K, Allali-Hassani A, Martin F, Zeng H, Loppnau P, Vedadi M, Bochkarev A, Plotnikov AN, Cheng X
Proteins. 01.04.2007 67(1):198-208. doi: 10.1002/prot.21272
PMID: 17243178

Structural basis of inhibition of the human NAD+-dependent deacetylase SIRT5 by suramin.
Schuetz A, Min J, Antoshenko T, Wang CL, Allali-Hassani A, Dong A, Loppnau P, Vedadi M, Bochkarev A, Sternglanz R, Plotnikov AN
Structure. 16.03.2007 15(3):377-89. doi: 10.1016/j.str.2007.02.002
PMID: 17355872

Analysis of binding site similarity, small-molecule similarity and experimental binding profiles in the human cytosolic sulfotransferase family.
Najmanovich RJ, Allali-Hassani A, Morris RJ, Dombrovsky L, Pan PW, Vedadi M, Plotnikov AN, Edwards A, Arrowsmith C, Thornton JM
Bioinformatics. 15.01.2007 23(2):e104-9. doi: 10.1093/bioinformatics/btl292
PMID: 17237076

2006

Genome-scale protein expression and structural biology of Plasmodium falciparum and related Apicomplexan organisms.
Vedadi M, Lew J, Artz J, Amani M, Zhao Y, Dong A, Wasney GA, Gao M, Hills T, Brokx S, Qiu W, Sharma S, Diassiti A, Alam Z, Melone M, Mulichak A, Wernimont A, Bray J, Loppnau P, Plotnikova O, Newberry K, Sundararajan E, Houston S, Walker J, Tempel W, Bochkarev A, Kozieradzki I, Edwards A, Arrowsmith C, Roos D, Kain K, Hui R
Mol. Biochem. Parasitol.. 13.11.2006 151(1):100-10. doi: 10.1016/j.molbiopara.2006.10.011
PMID: 17125854

Screening for ligands using a generic and high-throughput light-scattering-based assay.
Senisterra GA, Markin E, Yamazaki K, Hui R, Vedadi M, Awrey DE
J Biomol Screen. 07.11.2006 11(8):940-8. doi: 10.1177/1087057106294699
PMID: 17092916

Chemical screening methods to identify ligands that promote protein stability, protein crystallization, and structure determination.
Vedadi M, Niesen FH, Allali-Hassani A, Fedorov OY, Finerty PJ, Wasney GA, Yeung R, Arrowsmith C, Ball LJ, Berglund H, Hui R, Marsden BD, Nordlund P, Sundstrom M, Weigelt J, Edwards AM
Proc. Natl. Acad. Sci. U.S.A.. 24.10.2006 103(43):15835-40. doi: 10.1073/pnas.0605224103
PMID: 17035505

Structural basis for molecular recognition and presentation of histone H3 by WDR5.
Schuetz A, Allali-Hassani A, Martín F, Loppnau P, Vedadi M, Bochkarev A, Plotnikov AN, Arrowsmith CH, Min J
EMBO J.. 20.09.2006 25(18):4245-52. doi: 10.1038/sj.emboj.7601316
PMID: 16946699

SDR goes SGC: A structural genomics initiative
Oppermann, U; Kavanagh, K; Guo, KD; Ng, S; Lukacik, P; Wu, XQ; Dubinina, E; Shafqat, N; Bray, J; Marsden, B; Sharma, S; Vedadi, M; von Delft, F; Sundstrom, M;
. 01.01.2006 12:235-241. doi:
PMID:

2005

Enzymology and Molecular Biology of Carbonyl Metabolism
Marsden, B; Oppermann, U; Kavanagh, K; Guo, K; Ng, S; Lukacik, P; Wu, X; Dubinia, E; Shafqat, N; Bray, J; Sharma, S; Vedadi, M;
. 11.11.2005 12:-. doi:
PMID:

2004

Crystal structure and biophysical characterization of human GDP-D-mannose 4, 6-dehydratase
Vedadi, M; Walker, JR; Sharma, S; Houston, SA; Wasney, GA; Loppnau, P; Oppermann, U;
Protein Science. 01.8.2004 13:81-82. doi:
PMID:

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