Protein Production Workshop 2016

Dates: 6th - 8th June, 2016
Venue: Old Road Campus Research Building and Target Discovery Institute
Headington, Oxford, UK

Details

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The Workshop

The SGC in Oxford is hosting the third protein production workshop on soluble and membrane proteins, which will include one day of small group lab-based teaching sessions from SGC staff and 1.5 days of talks by international experts in the field of protein production. This course aims to provide training for young researchers and experienced protein scientists in the key skill that underpins any protein based structure/function work, i.e. production of good quality protein, assessment of the quality of the protein and how to use these proteins for crystallisation and functional studies. This meeting will provide hands on experience in protein based techniques, methodology talks and question and answer sessions where experts will discuss their technologies and suggest solutions for protein production issues raised by participants.

Organisers : Nicola Burgess-Brown and Liz Carpenter, SGC, University of Oxford

Registration closing date: 15th April

Programme

Protein Production Workshop Programme

6^th – 8^th June, 2016

SGC, ORCRB and TDI Buildings, Oxford, UK

Download program

Day 1	Lab Workshop (ORCRB, SGC labs, 6th June, 2016)
9:15-9:30	Registration in ORCRB atrium
9:30-12:30	Workshops session 1
12:30-13:30	Lunch (ORCRB atrium)
13:30-16:30	Workshops session 2
Day 2	Speaker talks (TDI, Basement Seminar Room, 7^th June, 2016)
8:30-9:00	Registration and coffee
9:00-9:10	Welcome Nicola Burgess-Brown
9:10-9:35	Session 1 - Challenging Proteins (Chair – Nicola Burgess-Brown) Opher Gileadi (SGC, Brazil) “*Producing challenging proteins*”
9:35-10:00	Ross Overman (AstraZeneca, UK) “*Protein Science at AstraZeneca; Novel Approaches to Tricky Targets*”
10:00-10:25	Radu Aricescu (STRUBI, University of Oxford) “*Protein production in mammalian cells*”
10:25-10:45	Discussion lead by the chair
10:45-11:15	Break
11:15-11:40	Session 2 – Antibodies/Binders (Chair – Imre Berger) Susanne Gräslund (SGC, Stockholm) “*HT production of high-quality antigens and recombinant antibodies*”
11:40-12:05	Jonas Schaefer (University of Zurich, Switzerland) “*Next-Generation Binder Discovery – Multipurpose DARPins for Innovative Applications*”
12:05-12:30	Joanne Nettleship (OPPF, UK) “*Streamlined production of antibody fragments using mammalian cells for structural studies*”
12:30-12:50	Discussion lead by the chair
13:00-14:00	Lunch (ORCRB atrium)
14:00-14:25	Session 3 – Baculovirus Expression Systems (Chair – James Love) Bob Possee (OET Ltd, Oxford) “*Hybrid gene promoters, supercharged viruses and genetic stability*”
14:25-14:50	Imre Berger (University of Bristol, UK) “*Baculovirus expression: Old dog, new tricks*”
14:50-15:15	Dominic Tisi (Astex, UK) “*Protein production and biophysical characterisation of the epigenetic oncogene mmSET*”
15:15-15:35	Discussion lead by the chair
15:35-16:00	Break
16:00-16:25	Session 4 – HTP Screening (Chair – Renaud Vincentelli) Darren Hart (IBS, France) “*Engineering of proteins and their interactions using random library approaches*”
16:25-16:50	James Love (DNA2.0, US) “*Making the most from mammalian protein production*”
16:50-17:15	Lei Kai (Max Planck Institute of Biochemistry, Germany) “*The application of high-throughput cell free platform*”
17:15-17:40	Bob Stroud (UCSF, US) “*Dialectics of a yeast pipeline for eukaryotic membrane proteins*”
17:40-18:00	Discussion lead by the chair
	End of Day 2 Buffet and Drinks at Al-Andalus Tapas Bar starting at 19:00 (Directions provided at end)
Day 3	Speaker talks (TDI, Basement Seminar Room, 8th June, 2016)
9:00-9:25	Session 1 – Protein Characterisation/Quality Control (Chair – Ross Overman) Renaud Vincentelli (AFMB, France) “*Quantifying affinities between all the PDZ domains and the Human Papillomavirus E6 oncoprotein by high-throughput « holdup » assay*”
9:25-9:50	Rod Chalk (SGC, Oxford) “*Mass Spectrometry of Proteins*”
9:50-10:15	Liz Carpenter (SGC, Oxford) “*Preparing membrane proteins for X-ray, EM, xFEL and functional studies*”
10:15-10:25	Karolina Peciak (AstraZeneca, UK) “*Design and production of NSD2 constructs to support identification of potent and selective inhibitors of MMSET with utility in multiple myeloma*”
10:25-10:35	Discussion lead by the chair
10:35-11:00	Break
11:00-11:25	Session 2 – Protein Production in Industry (Chair – Liz Carpenter) Joerg Weiske (Bayer, Germany) “*Protein Production @ Protein Technologies (Bayer)*”
11:25-11:50	Debra Brennan (Boehringer-Ingelheim, Germany) “Protein production with and without outsourcing”
11:50-12:15	Sandrine Thieffine (Evotec, UK) “*High-throughput Screening of Protein Constructs followed by Medium-throughput Scale-up*”
12:15-12:30	Discussion lead by the chair
12:30-13:30	Lunch (ORCRB atrium)
	End of Workshop

Directions to Al-Andalus Tapas Bar:

Al-Andalus Tapas Bar, 10 Little Clarendon Street, City Centre, Oxford OX1 2HP

Take the 4 a, b or c bus into town from Old Road bus stop

Registration

Registration form

Registration closing date: 15th April

Venue

Venues

Old Road Campus Research Building and Target Discovery Institute

Old Road Campus
Roosevelt Drive
Headington
Oxford OX3 7DQ
UK

Accommodation

Accommodation in Oxford

Oxford is well served by a large range of hotels and guest houses, many within walking distance of the symposium venue. We would like to recommend that you book your accommodation as soon as possible, as Oxford is a very popular destination.

Below is a list of links to help you to find your accommodation:

Travel

Travel

Travel by Air
Travel by Car
Travel by Bus
Travel by Train
Travel by Taxi

Directions

The venues are both situated at the Oxford University Old Road Campus (in Headington, next to the Churchill Hospital), roughly 2 miles east of Oxford city centre.

Address:

Old Road Campus
Roosevelt Drive
Headington
Oxford OX3 7DQ
UK

Travel by Air

Headington is well placed for airports in London and Birmingham.

London Heathrow
(approx. 40 miles away)
The Oxford Express X70 runs between Heathrow and Oxford and passes through central Headington. It departs from the Central Bus Station and Terminal 5 at Heathrow every 30 minutes and takes about an hour.

London Gatwick
(approx. 60 miles away)
The Oxford Express X80 runs between Gatwick and Oxford. It departs from Gatwick's North and South bus terminals every hour during the day and every two hours at night, taking just over two hours.

Birmingham Aiport
(approx. 60 miles away)
Travel to Oxford via M42 and M40 or by train directly from the aiport station (Birmingham International). Taxis and hire cars are available at the airport.

Travel by Car

IMPORTANT: Please note that parking is very difficult. Unless you have arranged a parking space you will need to use the pay and display car park at the Churchill Hospital which is often full. We recommend you use the Park and Ride facility instead.

From M40 (Westbound)

Exit at Junction 8 or Junction 8A. Take the A40 then A420 to central Headington and then follow the signs to the Churchill Hospital.

From M40 (Eastbound)

Exit at Junction 9 – take the A34 then A40 northern ring road to the Marston junction (B4150). Then turn left onto the B4495 towards Headington, follow the signs to the Churchill Hospital.

From the Oxford Ring Road

Leave the A4142 south-eastern ring road and the B4495 junction, then continue along The Slade towards Headington and follow the signs to the Churchill Hospital.

Park and Ride

Alternatively you can use the Thornhill Park and Ride buses (service 600), which leave every 20 minutes and stop at the Churchill Hospital (please visit herefor more information). From there, it is a short walk to the Old Road Campus Research Building. See the local ORCRB map for the pedestrial route (red) from the bus stop.

Travel by Bus

London Coaches

Frequent 24 hour direct services connect Oxford with London (peak times every 10-15 minutes).

The Oxford Express (X90) – service includes Victoria Coach Station, Grosvenor Gardens , Marble Arch, Baker Street/Gloucester Place and Hillingdon. Tel: 01865 785400

The Oxford Tube service includes Grosvenor Gardens , Marble Arch, Nottinghill Gate, Shepherd’s Bush and Hillingdon. Tel: 01865 772250

Local buses

There are frequent services to Windmill Road and Old Road from Oxford City Centre. See here for more specific details. Services are provided primarily by two companies: Oxford Bus Company and Stagecoach Oxford.

Travel by Train

Both ORCRB and TDI are just 2.5 miles from Oxford Station, which is serviced by frequent trains from London (Paddington) and many other parts of the country. Allow 15 minutes by taxi from the station forecourt and 30 minutes by bus. Refer to Oxford City bus information for travel from the train station to the venues.

National Rail Enquiries Telephone: 08457 48 49 50

For timetable information and prices visit: www.nationalrail.co.uk

Travel by Taxi

Private Taxis

There are a number of Companies which offer pre-bookable airport transfers. Rates range from £60-£70 for a single journey.