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Human Lamin B1

PDB Code 3HN9 Target Class Miscellaneous Target LMNB1 Alias n/a Disease Area/Function Date Deposited May 30 2009 Authors Xu C, Bian CB, Amaya MF, Bountra C, Weigelt J, Arrowsmith CH, Edwards AM, Bochkarev A, Min J Related Structure 3MOV

About this structure

Nuclear Lamins, also known as Class V intermediate filaments, are fibrous proteins providing structural function and transcriptional regulation in the cell nucleus. Nuclear lamins interact with membrane-associated proteins to form the nuclear lamina on the interior of the nuclear envelope. They are involved in the breakdown and reformation of the nuclear envelope during mitosis, as well as the positioning of nuclear pores.

B-type lamins are present in every cell. B type lamins, B1 and B2, are expressed from the LMNB1 and LMNB2 genes on 5q23 and 19q13, respectively. Lamin B1 contained a rod domain, and a C-terminal Ig-like domain. It is reported recently that Lamin B1 colocalized with PCNA via C-terminal Ig-like domain.

We have solved the structure of Lamin B1 Ig-like domain, which exhibits a beta sandwich fold. Two beta sheets formed the whole fold, while One sheet has five beta strands (433-438, 470-475, 478-483, , 527-532 and 538-545) and the other has four (442-447, 453-458, 495-500, 512-515). And two short bridge-like strands (464-465, 489-490) are present to link the 2 beta sheets at the top.

The large groove between two beta sheets consists of aromatic residues Tyr482, Tyr484, Tyr488 Trp515 and Trp521 at the base, which may play a potential role in intermolecular binding .