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human Homo sapiens heat shock 70kDa protein 2 (HSPA2)

PDB Code 3I33 Target Class ATPases Target HSPA2 Alias HSP70-2, HSP70-3, HSPA2 Disease Area/Function cancer Date Deposited Jun 30 2009 Authors Wisniewska, M., Karlberg, T., Arrowsmith, C.H., Berglund, H., Bountra, C., Collins, R., Edwards, A.M., Flodin, S., Flores, A., Graslund, S., Hammarstrom, M., Johansson, A., Johansson, I., Kotzsch, A., Kotenyova, T., Moche, M., Nielsen, T.K., Nordlund, P., Nyman, T., Persson, C., Roos, A.K., Sagemark, J., Schutz, P., Siponen, M.I., Thorsell, A.G., Tresaugues, L., Van Den Berg, S., Weigelt, J., Welin, M., Schueler, H., Structural Genomics Consortium (SGC)

About this structure

The human heat shock-70 like protein-2 (HSPA2; also known as Hsp70-2) is a constitutively expressed cytosolic member of the Hsp70 family, with detectable protein in most tissues and high expression levels in testis and skeletal muscle (1) and altered expression patterns in certain tumors (2-3). By analogy with other Hsp70 proteins, HSPA2 stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins.

We have previously determined the crystal structures of the ATPase domains of Hsp70 isoforms HSPA6 (PDB: 3fe1) and HSPA1L (PDB: 3gdq). Here, the structure of the corresponding part of HSPA2 (residues P7 – I384) at 1.2 Å resolution is presented. Our structure shows the protein with one magnesium ion and the products of ATP hydrolysis (one molecule of ADP and one inorganic phosphate) bound in the cleft between the two major lobes of the ATPase domain.

References

  1. Milner CM, Campbell RD (1992) Polymorphic analysis of the three MHC-linked HSP70 genes. Immunogenetics 36: 357-362
  2. Cutcliffe C, Kersey D, Huang CC, Zeng Y, Walterhouse D, Perlman EJ (2005) Clear cell sarcoma of the kidney: up-regulation of neural markers with activation of the sonic hedgehog and Akt pathway. Clin Cancer Res 11:7986-7994
  3. Gashaw I (2005) Gene signatures of testicular seminoma with emphasis on expression of ets variant gene 4. Cell Mol. Life Sci. 62: 2359-2368