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DNA binding domain of human Early B cell Factor 1
PDB Code 3LYR Target Class Miscellaneous Target EBF1 Alias COE1, EBF, EBF1, FLJ39389, FLJ41763, O/E-1, OLF1 Disease Area/Function chromatin and epigenetics Date Deposited Feb 28 2010 Authors Related Structure 3MQI
About this structure
The human EBF-family (Early B-cell factor, also known as the O/E or COE family) is a group of four transcription factors that play a variety of developmental roles [1]. They are e.g. involved in neuronal development, adipogenesis, and in B-cell lymphopoiesis where they crucial for B-cell maturation. In addition, EBF3 is found to be silenced in different cancer forms, in particular different brain tumours, and is suggested to act as a tumour suppressor [2].The structured part of the EBFs is composed of a DNA binding domain, a TIG/IPT domain, and a helical region. The EBF proteins show high homology within the family over the whole structured part, and bind to specific DNA response elements as homo or hetero dimers. The dimerisation motif it is suggested to reside mainly in the atypical helix-loop-helix motif while the main trans-activation region is found in the C-terminus. There is previously no structural information available for the EBF proteins.
We have solved the DNA binding domain of human EBF1 to 2.5 Å resolution (3LYR.pdb). Despite low sequence similarity the structure reveals a resemblance to the DNA binding domains of the Rel homology superfamily. A Zn-ion is found in the postulated Zn-knuckle, a region important for specific DNA binding and transcriptional activation [3].
