Susanne Gräslund

Optimizing Production of Antigens and Fabs in the Context of Generating Recombinant Antibodies to Human Proteins.

Zhong N, Loppnau P, Seitova A, Ravichandran M, Fenner M, Jain H, Bhattacharya A, Hutchinson A, Paduch M, Lu V, Olszewski M, Kossiakoff AA, Dowdell E, Koide A, Koide S, Huang H, Nadeem V, Sidhu SS, Greenblatt JF, Marcon E, Arrowsmith CH, Edwards AM, Gräslund S
PLoS ONE. 2015 10(10):e0139695. doi: 10.1371/journal.pone.0139695
PMID: 26437229

Assessment of a method to characterize antibody selectivity and specificity for use in immunoprecipitation.

Marcon E, Jain H, Bhattacharya A, Guo H, Phanse S, Pu S, Byram G, Collins BC, Dowdell E, Fenner M, Guo X, Hutchinson A, Kennedy JJ, Krastins B, Larsen B, Lin ZY, Lopez MF, Loppnau P, Miersch S, Nguyen T, Olsen JB, Paduch M, Ravichandran M, Seitova A, Vadali G, Vogelsang MS, Whiteaker JR, Zhong G, Zhong N, Zhao L, Aebersold R, Arrowsmith CH, Emili A, Frappier L, Gingras AC, Gstaiger M, Paulovich AG, Koide S, Kossiakoff AA, Sidhu SS, Wodak SJ, Gräslund S, Greenblatt JF, Edwards AM
Nat. Methods. 2015 . doi: 10.1038/nmeth.3472
PMID: 26121405

Lysine Demethylase KDM4A Associates with Translation Machinery and Regulates Protein Synthesis.

Van Rechem C, Black JC, Boukhali M, Aryee MJ, Graslund S, Haas W, Benes CH, Whetstine JR
Cancer Discov. 2015 . doi: 10.1158/2159-8290.CD-14-1326
PMID: 25564516

A roadmap to generate renewable protein binders to the human proteome.

Colwill K, Gräslund S
Nat. Methods. 2011 8(7):551-8. doi: 10.1038/nmeth.1607
PMID: 21572409

Protein production and purification.

Gräslund S, Nordlund P, Weigelt J, Hallberg BM, Bray J, Gileadi O, Knapp S, Oppermann U, Arrowsmith C, Hui R, Ming J, dhe-Paganon S, Park HW, Savchenko A, Yee A, Edwards A, Vincentelli R, Cambillau C, Kim R, Kim SH, Rao Z, Shi Y, Terwilliger TC, Kim CY, Hung LW, Waldo GS, Peleg Y, Albeck S, Unger T, Dym O, Prilusky J, Sussman JL, Stevens RC, Lesley SA, Wilson IA, Joachimiak A, Collart F, Dementieva I, Donnelly MI, Eschenfeldt WH, Kim Y, Stols L, Wu R, Zhou M, Burley SK, Emtage JS, Sauder JM, Thompson D, Bain K, Luz J, Gheyi T, Zhang F, Atwell S, Almo SC, Bonanno JB, Fiser A, Swaminathan S, Studier FW, Chance MR, Sali A, Acton TB, Xiao R, Zhao L, Ma LC, Hunt JF, Tong L, Cunningham K, Inouye M, Anderson S, Janjua H, Shastry R, Ho CK, Wang D, Wang H, Jiang M, Montelione GT, Stuart DI, Owens RJ, Daenke S, Schütz A, Heinemann U, Yokoyama S, Büssow K, Gunsalus KC
Nat. Methods. 2008 5(2):135-46. doi: 10.1038/nmeth.f.202
PMID: 18235434

The use of systematic N- and C-terminal deletions to promote production and structural studies of recombinant proteins.

Gräslund S, Sagemark J, Berglund H, Dahlgren LG, Flores A, Hammarström M, Johansson I, Kotenyova T, Nilsson M, Nordlund P, Weigelt J
Protein Expr. Purif.. 2007 58(2):210-21. doi: 10.1016/j.pep.2007.11.008
PMID: 18171622

2020

Screening and Production of Recombinant Human Proteins: Protein Production in E. coli.

Burgess-Brown NA, Mahajan P, Strain-Damerell C, Fernandez-Cid A, Gileadi O, Gräslund S
Methods Mol Biol. 31.10.2020 2199:45-66. doi: 10.1007/978-1-0716-0892-0_4
PMID: 33125644

Generation and validation of recombinant antibodies to study human aminoacyl tRNA synthetases.

Preger C, Wigren E, Ossipova E, Marks C, Lengqvist J, Hofström C, Andersson O, Jakobsson PJ, Gräslund S, Persson H
J. Biol. Chem.. 14.8.2020 . doi: 10.1074/jbc.RA120.012893
PMID: 32817337

2019

Strategies to improve scFvs as crystallization chaperones suggested by analysis of a complex with the human PHD-bromodomain SP140

Fairhead M, Preger C, Wigren E, Strain-Damerell C, Ossipova E, Ye M, Makola M, Burgess-Brown NA, Persson H, von Delft F, Gräslund S
. 12.09.2019 . doi: 10.1101/767376
PMID:

2018

Patients with anti-Jo1 antibodies display a characteristic IgG Fc-glycan profile which is further enhanced in anti-Jo1 autoantibodies.

Fernandes-Cerqueira C, Renard N, Notarnicola A, Wigren E, Gräslund S, Zubarev RA, Lundberg IE, Lundström SL
Sci Rep. 18.12.2018 8(1):17958. doi: 10.1038/s41598-018-36395-z
PMID: 30560888

A DNA-Encoded Library of Chemical Compounds Based on Common Scaffolding Structures Reveals the Impact of Ligand Geometry on Protein Recognition.

Favalli N, Biendl S, Hartmann M, Piazzi J, Sladojevich F, Gräslund S, Brown PJ, Näreoja K, Schüler H, Scheuermann J, Franzini R, Neri D
ChemMedChem. 1.6.2018 . doi: 10.1002/cmdc.201800193
PMID: 29856130

2017

In Vivo Biotinylation of Antigens in E. coli.

Gräslund S, Savitsky P, Müller-Knapp S
Methods Mol. Biol.. 5.5.2017 1586:337-344. doi: 10.1007/978-1-4939-6887-9_22
PMID: 28470616

Antibody Validation by Immunoprecipitation Followed by Mass Spectrometry Analysis.

Persson H, Preger C, Marcon E, Lengqvist J, Gräslund S
Methods Mol. Biol.. 4.3.2017 1575:175-187. doi: 10.1007/978-1-4939-6857-2_10
PMID: 28255880

2016

Apollo-NADP(+): a spectrally tunable family of genetically encoded sensors for NADP(.)

Cameron WD, Bui CV, Hutchinson A, Loppnau P, Gräslund S, Rocheleau JV
Nat. Methods. 15.2.2016 . doi: 10.1038/nmeth.3764
PMID: 26878383

2015

Streamlining the Pipeline for Generation of Recombinant Affinity Reagents by Integrating the Affinity Maturation Step.

Huang R, Gorman KT, Vinci CR, Dobrovetsky E, Gräslund S, Kay BK
Int J Mol Sci. 6.10.2015 16(10):23587-603. doi: 10.3390/ijms161023587
PMID: 26437402

Optimizing Production of Antigens and Fabs in the Context of Generating Recombinant Antibodies to Human Proteins.

Zhong N, Loppnau P, Seitova A, Ravichandran M, Fenner M, Jain H, Bhattacharya A, Hutchinson A, Paduch M, Lu V, Olszewski M, Kossiakoff AA, Dowdell E, Koide A, Koide S, Huang H, Nadeem V, Sidhu SS, Greenblatt JF, Marcon E, Arrowsmith CH, Edwards AM, Gräslund S
PLoS ONE. 5.10.2015 10(10):e0139695. doi: 10.1371/journal.pone.0139695
PMID: 26437229

A High Through-put Platform for Recombinant Antibodies to Folded Proteins.

Hornsby M, Paduch M, Miersch S, Sääf A, Matsuguchi T, Lee B, Wypisniak K, Doak A, King D, Usatyuk S, Perry K, Lu V, Thomas W, Luke J, Goodman J, Hoey RJ, Lai D, Griffin C, Li Z, Vizeacoumar FJ, Dong D, Campbell E, Anderson S, Zhong N, Gräslund S, Koide S, Moffat J, Sidhu S, Kossiakoff A, Wells J
Mol. Cell Proteomics. 19.8.2015 . doi: 10.1074/mcp.O115.052209
PMID: 26290498

Assessment of a method to characterize antibody selectivity and specificity for use in immunoprecipitation.

Structural Basis for the Specificity of Human NUDT16 and Its Regulation by Inosine Monophosphate.

Trésaugues L, Lundbäck T, Welin M, Flodin S, Nyman T, Silvander C, Gräslund S, Nordlund P
PLoS ONE. 29.6.2015 10(6):e0131507. doi: 10.1371/journal.pone.0131507
PMID: 26121039

Tankyrase 1 Inhibitors with Drug-like Properties Identified by Screening a DNA-Encoded Chemical Library.

Samain F, Ekblad T, Mikutis G, Zhong N, Zimmermann M, Nauer A, Bajic D, Decurtins W, Scheuermann J, Brown PJ, Hall J, Gräslund S, Schüler H, Neri D, Franzini RM
J. Med. Chem.. 10.6.2015 . doi: 10.1021/acs.jmedchem.5b00432
PMID: 26061013

Identification of Structure-Activity Relationships from Screening a Structurally Compact DNA-Encoded Chemical Library.

Franzini RM, Ekblad T, Zhong N, Wichert M, Decurtins W, Nauer A, Zimmermann M, Samain F, Scheuermann J, Brown PJ, Hall J, Gräslund S, Schüler H, Neri D
Angew. Chem. Int. Ed. Engl.. 3.2.2015 . doi: 10.1002/anie.201410736
PMID: 25650139

Lysine Demethylase KDM4A Associates with Translation Machinery and Regulates Protein Synthesis.

Van Rechem C, Black JC, Boukhali M, Aryee MJ, Graslund S, Haas W, Benes CH, Whetstine JR
Cancer Discov. 6.1.2015 . doi: 10.1158/2159-8290.CD-14-1326
PMID: 25564516

2014

Recombinant renewable polyclonal antibodies.

Ferrara F, D'Angelo S, Gaiotto T, Naranjo L, Tian H, Gräslund S, Dobrovetsky E, Hraber P, Lund-Johansen F, Saragozza S, Sblattero D, Kiss C, Bradbury AR
MAbs. 20.12.2014 . doi: 10.4161/19420862.2015.989047
PMID: 25530082

Human-chromatin-related protein interactions identify a demethylase complex required for chromosome segregation.

Marcon E, Ni Z, Pu S, Turinsky AL, Trimble SS, Olsen JB, Silverman-Gavrila R, Silverman-Gavrila L, Phanse S, Guo H, Zhong G, Guo X, Young P, Bailey S, Roudeva D, Zhao D, Hewel J, Li J, Gräslund S, Paduch M, Kossiakoff AA, Lupien M, Emili A, Wodak SJ, Greenblatt J
Cell Rep. 10.7.2014 8(1):297-310. doi: 10.1016/j.celrep.2014.05.050
PMID: 24981860

Structural basis for phosphoinositide substrate recognition, catalysis, and membrane interactions in human inositol polyphosphate 5-phosphatases.

Trésaugues L, Silvander C, Flodin S, Welin M, Nyman T, Gräslund S, Hammarström M, Berglund H, Nordlund P
Structure. 6.5.2014 22(5):744-55. doi: 10.1016/j.str.2014.01.013
PMID: 24704254

A Basic Post-SET Extension of NSDs Is Essential for Nucleosome Binding In Vitro.

Allali-Hassani A, Kuznetsova E, Hajian T, Wu H, Dombrovski L, Li Y, Gräslund S, Arrowsmith CH, Schapira M, Vedadi M
J Biomol Screen. 4.3.2014 . doi: 10.1177/1087057114525854
PMID: 24595546

2013

Medium-Throughput Production of Recombinant Human Proteins: Protein Production in E. coli.

Burgess-Brown NA, Mahajan P, Strain-Damerell C, Gileadi O, Gräslund S
Methods Mol. Biol.. 10.11.2013 1091:73-94. doi: 10.1007/978-1-62703-691-7_5
PMID: 24203325

Substrate Specificity and Oligomerization of Human GMP Synthetase.

Welin M, Lehtiö L, Johansson A, Flodin S, Nyman T, Trésaugues L, Hammarström M, Gräslund S, Nordlund P
J. Mol. Biol.. 28.6.2013 . doi: 10.1016/j.jmb.2013.06.032
PMID: 23816837

2012

Expressing the human proteome for affinity proteomics: optimising expression of soluble protein domains and in vivo biotinylation.

Keates T, Cooper CD, Savitsky P, Allerston CK, Phillips C, Hammarström M, Daga N, Berridge G, Mahajan P, Burgess-Brown NA, Müller S, Gräslund S, Gileadi O
N Biotechnol. 15.6.2012 29(5):515-25. doi: 10.1016/j.nbt.2011.10.007
PMID: 22027370

Pan-pathway based interaction profiling of FDA-approved nucleoside and nucleobase analogs with enzymes of the human nucleotide metabolism.

Egeblad L, Welin M, Flodin S, Gräslund S, Wang L, Balzarini J, Eriksson S, Nordlund P
PLoS ONE. 25.5.2012 7(5):e37724. doi: 10.1371/journal.pone.0037724
PMID: 22662200

2011

Recombinant protein quality evaluation: proposal for a minimal information standard.

Buckle AM, Bate MA, Androulakis S, Cinquanta M, Basquin J, Bonneau F, Chatterjee DK, Cittaro D, Gräslund S, Gruszka A, Page R, Suppmann S, Wheeler JX, Agostini D, Taussig M, Taylor CF, Bottomley SP, Villaverde A, de Marco A
Stand Genomic Sci. 30.11.2011 5(2):195-7. doi: 10.4056/sigs.1834511
PMID: 22180821

A roadmap to generate renewable protein binders to the human proteome.

Colwill K, Gräslund S
Nat. Methods. 15.5.2011 8(7):551-8. doi: 10.1038/nmeth.1607
PMID: 21572409

A secretory system for bacterial production of high-profile protein targets.

Kotzsch A, Vernet E, Hammarström M, Berthelsen J, Weigelt J, Gräslund S, Sundström M
Protein Sci.. 11.2.2011 20(3):597-609. doi: 10.1002/pro.593
PMID: 21308845

2010

Towards proteome scale antibody selections using phage display.

Mersmann M, Meier D, Mersmann J, Helmsing S, Nilsson P, Gräslund S, Colwill K, Hust M, Dübel S
N Biotechnol. 31.5.2010 27(2):118-28. doi: 10.1016/j.nbt.2009.10.007
PMID: 19883803

Generating a panel of highly specific antibodies to 20 human SH2 domains by phage display

Pershad K, Pavlovic JD, Gräslund S, Nilsson P, Colwill K, Karatt-Vellatt A, Schofield DJ, Dyson MR, Pawson T, Kay BK, McCafferty J.
Protein Eng Des Sel.. 04.23.2010 (4):279-88. doi: 10.1093/protein/gzq003
PMID: 20164216

Generating a panel of highly specific antibodies to 20 human SH2 domains by phage display.

Pershad K, Pavlovic JD, Gräslund S, Nilsson P, Colwill K, Karatt-Vellatt A, Schofield DJ, Dyson MR, Pawson T, Kay BK, McCafferty J
Protein Eng. Des. Sel.. 17.2.2010 23(4):279-88. doi: 10.1093/protein/gzq003
PMID: 20164216

2009

Crystal structure of human diphosphoinositol phosphatase 1.

Thorsell AG, Persson C, Gräslund S, Hammarström M, Busam RD, Hallberg BM
Proteins. 9.7.2009 77(1):242-6. doi: 10.1002/prot.22489
PMID: 19585659

2008

A pilot project to generate affinity reagents to human proteins.

Uhlen M, Gräslund S, Sundström M
Nat. Methods. 1.10.2008 5(10):854-5. doi: 10.1038/nmeth1008-854
PMID: 18825127

The crystal structure of human cleavage and polyadenylation specific factor-5 reveals a dimeric Nudix protein with a conserved catalytic site.

Trésaugues L, Stenmark P, Schüler H, Flodin S, Welin M, Nyman T, Hammarström M, Moche M, Gräslund S, Nordlund P
Proteins. 4.9.2008 73(4):1047-52. doi: 10.1002/prot.22198
PMID: 18767156

The structure of the PP2A regulatory subunit B56 gamma: the remaining piece of the PP2A jigsaw puzzle.

Magnusdottir A, Stenmark P, Flodin S, Nyman T, Kotenyova T, Gräslund S, Ogg D, Nordlund P
Proteins. 12.7.2008 74(1):212-21. doi: 10.1002/prot.22150
PMID: 18618707

Structural and biophysical characterization of human myo-inositol oxygenase.

Thorsell AG, Persson C, Voevodskaya N, Busam RD, Hammarström M, Gräslund S, Gräslund A, Hallberg BM
J. Biol. Chem.. 30.5.2008 283(22):15209-16. doi: 10.1074/jbc.M800348200
PMID: 18364358

Protein production and purification.

Gräslund S, Nordlund P, Weigelt J, Hallberg BM, Bray J, Gileadi O, Knapp S, Oppermann U, Arrowsmith C, Hui R, Ming J, dhe-Paganon S, Park HW, Savchenko A, Yee A, Edwards A, Vincentelli R, Cambillau C, Kim R, Kim SH, Rao Z, Shi Y, Terwilliger TC, Kim CY, Hung LW, Waldo GS, Peleg Y, Albeck S, Unger T, Dym O, Prilusky J, Sussman JL, Stevens RC, Lesley SA, Wilson IA, Joachimiak A, Collart F, Dementieva I, Donnelly MI, Eschenfeldt WH, Kim Y, Stols L, Wu R, Zhou M, Burley SK, Emtage JS, Sauder JM, Thompson D, Bain K, Luz J, Gheyi T, Zhang F, Atwell S, Almo SC, Bonanno JB, Fiser A, Swaminathan S, Studier FW, Chance MR, Sali A, Acton TB, Xiao R, Zhao L, Ma LC, Hunt JF, Tong L, Cunningham K, Inouye M, Anderson S, Janjua H, Shastry R, Ho CK, Wang D, Wang H, Jiang M, Montelione GT, Stuart DI, Owens RJ, Daenke S, Schütz A, Heinemann U, Yokoyama S, Büssow K, Gunsalus KC
Nat. Methods. 1.2.2008 5(2):135-46. doi: 10.1038/nmeth.f.202
PMID: 18235434

2007

In situ proteolysis for protein crystallization and structure determination.

Dong A, Xu X, Edwards AM; Midwest Center for Structural Genomics; Structural Genomics Consortium, Chang C, Chruszcz M, Cuff M, Cymborowski M, Di Leo R, Egorova O, Evdokimova E, Filippova E, Gu J, Guthrie J, Ignatchenko A, Joachimiak A, Klostermann N, Kim Y, Korniyenko Y, Minor W, Que Q, Savchenko A, Skarina T, Tan K, Yakunin A, Yee A, Yim V, Zhang R, Zheng H, Akutsu M, Arrowsmith C, Avvakumov GV, Bochkarev A, Dahlgren LG, Dhe-Paganon S, Dimov S, Dombrovski L, Finerty P Jr, Flodin S, Flores A, Gräslund S, Hammerström M, Herman MD, Hong BS, Hui R, Johansson I, Liu Y, Nilsson M, Nedyalkova L, Nordlund P, Nyman T, Min J, Ouyang H, Park HW, Qi C, Rabeh W, Shen L, Shen Y, Sukumard D, Tempel W, Tong Y, Tresagues L, Vedadi M, Walker JR, Weigelt J, Welin M, Wu H, Xiao T, Zeng H, Zhu H.
Nat Methods. 01.12.2007 4(12):1019-21. doi:
PMID: 17982461

The use of systematic N- and C-terminal deletions to promote production and structural studies of recombinant proteins.

Gräslund S, Sagemark J, Berglund H, Dahlgren LG, Flores A, Hammarström M, Johansson I, Kotenyova T, Nilsson M, Nordlund P, Weigelt J
Protein Expr. Purif.. 22.11.2007 58(2):210-21. doi: 10.1016/j.pep.2007.11.008
PMID: 18171622

In situ proteolysis for protein crystallization and structure determination.

Dong A, Xu X, Edwards AM, Chang C, Chruszcz M, Cuff M, Cymborowski M, Di Leo R, Egorova O, Evdokimova E, Filippova E, Gu J, Guthrie J, Ignatchenko A, Joachimiak A, Klostermann N, Kim Y, Korniyenko Y, Minor W, Que Q, Savchenko A, Skarina T, Tan K, Yakunin A, Yee A, Yim V, Zhang R, Zheng H, Akutsu M, Arrowsmith C, Avvakumov GV, Bochkarev A, Dahlgren LG, Dhe-Paganon S, Dimov S, Dombrovski L, Finerty P, Flodin S, Flores A, Gräslund S, Hammerström M, Herman MD, Hong BS, Hui R, Johansson I, Liu Y, Nilsson M, Nedyalkova L, Nordlund P, Nyman T, Min J, Ouyang H, Park HW, Qi C, Rabeh W, Shen L, Shen Y, Sukumard D, Tempel W, Tong Y, Tresagues L, Vedadi M, Walker JR, Weigelt J, Welin M, Wu H, Xiao T, Zeng H, Zhu H
Nat. Methods. 4.11.2007 4(12):1019-21. doi: 10.1038/nmeth1118
PMID: 17982461

Crystal structure of human cytosolic 5'-nucleotidase II: insights into allosteric regulation and substrate recognition.

Walldén K, Stenmark P, Nyman T, Flodin S, Gräslund S, Loppnau P, Bianchi V, Nordlund P
J. Biol. Chem.. 15.6.2007 282(24):17828-36. doi: 10.1074/jbc.M700917200
PMID: 17405878

Crystal structure of human inosine triphosphatase. Substrate binding and implication of the inosine triphosphatase deficiency mutation P32T.

Stenmark P, Kursula P, Flodin S, Gräslund S, Landry R, Nordlund P, Schüler H
J. Biol. Chem.. 2.2.2007 282(5):3182-7. doi: 10.1074/jbc.M609838200
PMID: 17138556

Principal Investigator

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2007