Opher Gileadi

Principal Investigator

University of Oxford
+44 (0)1865 617572

Opher Gileadi studied at the Hebrew University. He did his post-doc at Stanford, then led a research group at the Weizmann Institute. Opher has studied the biochemical basis of gene expression and of DNA repair, using a wide range of approaches including differential mRNA analysis in human trophoblast cells, studies of transcription factors using purified systems and yeast genetics, and single-molecule analyses of protein:DNA interactions.

Research Areas

At the SGC, Opher established the Biotechnology group, developing methods for high-throughput production of human proteins for crystallization. He now heads the Genome Integrity and Neurodegeneration group, which studies DNA repair proteins and on proteins associated with human genetic disease. Recent work includes structures of RecQ DNA helicases and DNA cross-link repair endonucleases, and biochemical characterization of genes mutated in Carpenter’s syndrome and congenital dyserythropoietic anemia. We are also developing methods to intervene with DNA repair using small molecules such as the new inhibitors of the Bloom DNA helicase.

Publications

Structures of Two Melanoma-Associated Antigens Suggest Allosteric Regulation of Effector Binding.
Newman JA, Cooper CD, Roos AK, Aitkenhead H, Oppermann UC, Cho HJ, Osman R, Gileadi O
PLoS ONE. 2016 11(2):e0148762. doi: 10.1371/journal.pone.0148762
PMID: 26910052

Identification of LACTB2, a metallo-β-lactamase protein, as a human mitochondrial endoribonuclease.
Levy S, Allerston CK, Liveanu V, Habib MR, Gileadi O, Schuster G
Nucleic Acids Res.. 2016 . doi: 10.1093/nar/gkw050
PMID: 26826708

Human RECQ1 helicase-driven DNA unwinding, annealing, and branch migration: Insights from DNA complex structures.
Pike AC, Gomathinayagam S, Swuec P, Berti M, Zhang Y, Schnecke C, Marino F, von Delft F, Renault L, Costa A, Gileadi O, Vindigni A
Proc. Natl. Acad. Sci. U.S.A.. 2015 . doi: 10.1073/pnas.1417594112
PMID: 25831490

The structures of the SNM1A and SNM1B/Apollo nuclease domains reveal a potential basis for their distinct DNA processing activities.
Allerston CK, Lee SY, Newman JA, Schofield CJ, McHugh PJ, Gileadi O
Nucleic Acids Res.. 2015 . doi: 10.1093/nar/gkv1256
PMID: 26582912

Crystal structure of the Bloom's syndrome helicase indicates a role for the HRDC domain in conformational changes.
Newman JA, Savitsky P, Allerston CK, Bizard AH, Özer Ö, Sarlós K, Liu Y, Pardon E, Steyaert J, Hickson ID, Gileadi O
Nucleic Acids Res.. 2015 . doi: 10.1093/nar/gkv373
PMID: 25901030

Medium-throughput production of recombinant human proteins: ligation-independent cloning.
Strain-Damerell C, Mahajan P, Gileadi O, Burgess-Brown NA
Methods Mol. Biol.. 2013 1091:55-72. doi: 10.1007/978-1-62703-691-7_4
PMID: 24203324

Medium-throughput production of recombinant human proteins: protein production in insect cells.
Mahajan P, Strain-Damerell C, Gileadi O, Burgess-Brown NA
Methods Mol. Biol.. 2013 1091:95-121. doi: 10.1007/978-1-62703-691-7_6
PMID: 24203326

Crystal Structures of Malonyl-Coenzyme A Decarboxylase Provide Insights into Its Catalytic Mechanism and Disease-Causing Mutations.
Froese DS, Forouhar F, Tran TH, Vollmar M, Kim YS, Lew S, Neely H, Seetharaman J, Shen Y, Xiao R, Acton TB, Everett JK, Cannone G, Puranik S, Savitsky P, Krojer T, Pilka ES, Kiyani W, Lee WH, Marsden BD, von Delft F, Allerston CK, Spagnolo L, Gileadi O, Montelione GT, Oppermann U, Yue WW, Tong L
Structure. 2013 . doi: 10.1016/j.str.2013.05.001
PMID: 23791943

Homozygous mutations in a predicted endonuclease are a novel cause of congenital dyserythropoietic anemia type I.
Babbs, C; Roberts, NA; Sanchez-Pulido, L; McGowan, SJ; Ahmed, MR; Brown, JM; Sabry, MA; WGS500 Consortium, ; Bentley, DR; McVean, GA; Donnelly, P; Gileadi, O; Ponting, CP; Higgs, DR; Buckle, VJ;
Haematologica. 2013 98:1383-1387. doi: 10.3324/haematol.2013.089490
PMID: 23716552

A small molecule inhibitor of the BLM helicase modulates chromosome stability in human cells.
Nguyen GH, Dexheimer TS, Rosenthal AS, Chu WK, Singh DK, Mosedale G, Bachrati CZ, Schultz L, Sakurai M, Savitsky P, Abu M, McHugh PJ, Bohr VA, Harris CC, Jadhav A, Gileadi O, Maloney DJ, Simeonov A, Hickson ID
Chem. Biol.. 2013 20(1):55-62. doi: 10.1016/j.chembiol.2012.10.016
PMID: 23352139

Characterization of the human SNM1A and SNM1B/Apollo DNA repair exonucleases.
Sengerová B, Allerston CK, Abu M, Lee SY, Hartley J, Kiakos K, Schofield CJ, Hartley JA, Gileadi O, McHugh PJ
J. Biol. Chem.. 2012 287(31):26254-67. doi: 10.1074/jbc.M112.367243
PMID: 22692201

The PHD and chromo domains regulate the ATPase activity of the human chromatin remodeler CHD4.
Watson AA, Mahajan P, Mertens HD, Deery MJ, Zhang W, Pham P, Du X, Bartke T, Zhang W, Edlich C, Berridge G, Chen Y, Burgess-Brown NA, Kouzarides T, Wiechens N, Owen-Hughes T, Svergun DI, Gileadi O, Laue ED
J. Mol. Biol.. 2012 422(1):3-17. doi: 10.1016/j.jmb.2012.04.031
PMID: 22575888

Identification of macrodomain proteins as novel O-acetyl-ADP-ribose deacetylases.
Chen D, Vollmar M, Rossi MN, Phillips C, Kraehenbuehl R, Slade D, Mehrotra PV, von Delft F, Crosthwaite SK, Gileadi O, Denu JM, Ahel I
J. Biol. Chem.. 2011 286(15):13261-71. doi: 10.1074/jbc.M110.206771
PMID: 21257746

Human SNM1a and XPF-ERCC1 collaborate to initiate DNA interstrand cross-link repair
Wang, AT; Sengerová, B; Cattell, E; Inagawa, T; Hartley, JM; Kiakos, K; Burgess-Brown, NA; Swift, LP; Enzlin, JH; Schofield, CJ; Gileadi, O; Hartley, JA; McHugh, PJ;
Genes and Development. 2011 25:1859-1870. doi: 10.1101/gad.15699211
PMID:

High-performance liquid chromatography separation and intact mass analysis of detergent-solubilized integral membrane proteins.
Berridge G, Chalk R, D'Avanzo N, Dong L, Doyle D, Kim JI, Xia X, Burgess-Brown N, Deriso A, Carpenter EP, Gileadi O
Anal. Biochem.. 2011 410(2):272-80. doi: 10.1016/j.ab.2010.11.008
PMID: 21093405

A prominent β-hairpin structure in the winged-helix domain of RECQ1 is required for DNA unwinding and oligomer formation.
Lucic B, Zhang Y, King O, Mendoza-Maldonado R, Berti M, Niesen FH, Burgess-Brown NA, Pike AC, Cooper CD, Gileadi O, Vindigni A
Nucleic Acids Res.. 2010 39(5):1703-17. doi: 10.1093/nar/gkq1031
PMID: 21059676

High-throughput production of human proteins for crystallization: the SGC experience.
Savitsky P, Bray J, Cooper CD, Marsden BD, Mahajan P, Burgess-Brown NA, Gileadi O
J. Struct. Biol.. 2010 172(1):3-13. doi: 10.1016/j.jsb.2010.06.008
PMID: 20541610

Probing the structural basis of RecQ helicase function.
Vindigni A, Marino F, Gileadi O
Biophys. Chem.. 2010 149(3):67-77. doi: 10.1016/j.bpc.2010.03.012
PMID: 20392558

Structure of the human RECQ1 helicase reveals a putative strand-separation pin.
Pike AC, Shrestha B, Popuri V, Burgess-Brown N, Muzzolini L, Costantini S, Vindigni A, Gileadi O
Proc. Natl. Acad. Sci. U.S.A.. 2009 106(4):1039-44. doi: 10.1073/pnas.0806908106
PMID: 19151156

Codon optimization can improve expression of human genes in Escherichia coli: A multi-gene study.
Burgess-Brown NA, Sharma S, Sobott F, Loenarz C, Oppermann U, Gileadi O
Protein Expr. Purif.. 2008 59(1):94-102. doi: 10.1016/j.pep.2008.01.008
PMID: 18289875

Direct observation of RuvAB-catalyzed branch migration of single Holliday junctions (vol 101, pg 11605, 2004)
Amit, R; Gileadi, O; Stavans, J;
Proceedings of the National Academy of Sciences. 2006 103:12654-12654. doi:
PMID:

Direct observation of RuvAB-catalyzed branch migration of single Holliday junctions.
Amit, R; Gileadi, O; Stavans, J;
Proceedings of the National Academy of Sciences. 2004 101:11605-11610. doi: 10.1073/pnas.0404332101
PMID: 15292509

Scanning electron microscopy of cells and tissues under fully hydrated conditions.
Thiberge, S; Nechushtan, A; Sprinzak, D; Gileadi, O; Behar, V; Zik, O; Chowers, Y; Michaeli, S; Schlessinger, J; Moses, E;
Proceedings of the National Academy of Sciences. 2004 101:3346-3351. doi: 10.1073/pnas.0400088101
PMID: 14988502

Squid sperm to clam eggs: imaging wet samples in a scanning electron microscope.
Gileadi, O; Sabban, A;
Biological Bulletin. 2003 205:177-179. doi: 10.2307/1543239
PMID: 14583516

Compaction of single DNA molecules by histone-like binding protein HU.
Schnurr, B; Amit, R; Oppenheim, AB; Gileadi, O; Stavans, J;
Biophysical Journal. 2002 82:51A-51A. doi:
PMID:

2020

STAG1 vulnerabilities for exploiting cohesin synthetic lethality in STAG2-deficient cancers
van der Lelij P, Newman JA, Lieb S, Jude J, Katis V, Hoffmann T, Hinterndorfer M, Bader G, Kraut N, Pearson MA, Peters JM, Zuber J, Gileadi O, Petronczki M
Life science alliance. 2020 3:-. doi: 10.26508/lsa.202000725
PMID: 32467316

2019

Development of Pyridine-based Inhibitors for the Human Vaccinia-related Kinases 1 and 2
Serafim RAM, De Souza Gama FH, Dutra LA, Dos Reis CV, Vasconcelos SNS, Da Silva Santiago A, Takarada JE, Di Pillo F, Azevedo H, Mascarello A, Elkins JM, Massirer KB, Gileadi O, Guimarães CRW, Couñago RM
ACS Medicinal Chemistry Letters. 2019 10:1266-1271. doi: 10.1021/acsmedchemlett.9b00082
PMID: 31531195

A Chemical Probe Targeting AAK1 and BMP2K
Wells C, Counago R, Limas J, Almeida T, Cook J, Drewry D, Elkins J, Gileadi O, Kapadia N, Lorente-Macías Á, Pickett J, Riemen A, R. Ruela-de-Sousa R, Willson T, Zhang C, Zuercher W, Zutshi R, Axtman A
. 2019 . doi: 10.26434/chemrxiv.9756785.v1
PMID:

A Ubiquitin-Binding Domain that Binds a Structural Fold Distinct from that of Ubiquitin
Lim M, Newman JA, Williams HL, Masino L, Aitkenhead H, Gravard AE, Gileadi O, Svejstrup JQ
Structure. 2019 27:1316-1325.e6. doi: 10.1016/j.str.2019.05.003
PMID: 31204252

Streamlining the production of proteins for structural biology
Owens RJ, Gileadi O
Biophysical Reviews. 2019 11:533-534. doi: 10.1007/s12551-019-00565-8
PMID: 31250316

Role of protein structure in variant annotation: structural insight of mutations causing 6-pyruvoyl-tetrahydropterin synthase deficiency
Muniz JRC, Szeto NW-S, Frise R, Lee WH, Wang X-S, Thöny B, Himmelreich N, Blau N, Hsiao K-J, Liu T-T, Gileadi O, Oppermann U, Von Delft F, Yue WW, Tang NL-S
Pathology. 2019 51:274-280. doi: 10.1016/j.pathol.2018.11.011
PMID: 30853107

2018

Structural Analysis of Inhibitor Binding to CAMKK1 Identifies Features Necessary for Design of Specific Inhibitors
Santiago, ADS; Couñago, R; Zonzini Ramos, P; Godoi, PHC; Massirer, KB; Gileadi, O; Elkins, J;
Scientific Reports. 2018 :-. doi: 10.1101/292797
PMID: 30287839

The structural basis for forkhead box family specificity revealed by the crystal structure of human FOXN1 in complex with DNA
Newman, J; Aitkenhead, H; Gavard, A; Rota, I; Handel, A; Hollander, G; Gileadi, O;
. 2018 :-. doi: 10.1101/428011
PMID:

Structural Analysis of Inhibitor Binding to CAMKK1 Identifies Features Necessary for Design of Specific Inhibitors
Santiago, ADS; Couñago, R; Zonzini Ramos, P; Godoi, PHC; Massirer, KB; Gileadi, O; Elkins, J;
. 2018 :-. doi: 10.1101/292797
PMID:

Tryptophan-Mediated Interactions between Tristetraprolin and the CNOT9 Subunit Are Required for CCR4-NOT Deadenylase Complex Recruitment.
Bulbrook, D; Brazier, H; Mahajan, P; Kliszczak, M; Fedorov, O; Marchese, FP; Aubareda, A; Chalk, R; Picaud, S; Strain-Damerell, C; Filippakopoulos, P; Gileadi, O; Clark, AR; Yue, WW; Burgess-Brown, NA; Dean, JLE;
Journal of Molecular Biology. 2018 430:722-736. doi: 10.1016/j.jmb.2017.12.018
PMID: 29291391

2017

The SGC beyond structural genomics: redefining the role of 3D structures by coupling genomic stratification with fragment-based discovery.
Bradley AR, Echalier A, Fairhead M, Strain-Damerell C, Brennan P, Bullock AN, Burgess-Brown NA, Carpenter EP, Gileadi O, Marsden BD, Lee WH, Yue W, Bountra C, von Delft F
Essays Biochem.. 2017 61(5):495-503. doi: 10.1042/EBC20170051
PMID: 29118096

Structural characterization of human Vaccinia-Related Kinases (VRK) bound to small-molecule inhibitors identifies different P-loop conformations.
Couñago RM, Allerston CK, Savitsky P, Azevedo H, Godoi PH, Wells CI, Mascarello A, de Souza Gama FH, Massirer KB, Zuercher WJ, Guimarães CRW, Gileadi O
Sci Rep. 2017 7(1):7501. doi: 10.1038/s41598-017-07755-y
PMID: 28790404

Progress towards a public chemogenomic set for protein kinases and a call for contributions.
Drewry DH, Wells CI, Andrews DM, Angell R, Al-Ali H, Axtman AD, Capuzzi SJ, Elkins JM, Ettmayer P, Frederiksen M, Gileadi O, Gray N, Hooper A, Knapp S, Laufer S, Luecking U, Michaelides M, Müller S, Muratov E, Denny RA, Saikatendu KS, Treiber DK, Zuercher WJ, Willson TM
PLoS ONE. 2017 12(8):e0181585. doi: 10.1371/journal.pone.0181585
PMID: 28767711

Structural Characterization of Maize SIRK1 Kinase Domain Reveals an Unusual Architecture of the Activation Segment.
Aquino B, Couñago RM, Verza N, Ferreira LM, Massirer KB, Gileadi O, Arruda P
Front Plant Sci. 2017 8:852. doi: 10.3389/fpls.2017.00852
PMID: 28603531

Conformational Plasticity of the MAGE-A3 Protein as a Therapeutic Strategy in Multiple Myeloma
Osman, R; Cho, HJ; Gileadi, O;
Biophysical Journal. 2017 112:340A-340A. doi: 10.1016/j.bpj.2016.11.1840
PMID:

2016

Metalloprotease SPRTN/DVC1 Orchestrates Replication-Coupled DNA-Protein Crosslink Repair.
Vaz B, Popovic M, Newman JA, Fielden J, Aitkenhead H, Halder S, Singh AN, Vendrell I, Fischer R, Torrecilla I, Drobnitzky N, Freire R, Amor DJ, Lockhart PJ, Kessler BM, McKenna GW, Gileadi O, Ramadan K
Mol. Cell. 2016 64(4):704-719. doi: 10.1016/j.molcel.2016.09.032
PMID: 27871366

Cephalosporins inhibit human metallo β-lactamase fold DNA repair nucleases SNM1A and SNM1B/apollo.
Lee SY, Brem J, Pettinati I, Claridge TD, Gileadi O, Schofield CJ, McHugh PJ
Chem. Commun. (Camb.). 2016 . doi: 10.1039/c6cc00529b
PMID: 27121860

Structures of Two Melanoma-Associated Antigens Suggest Allosteric Regulation of Effector Binding.
Newman JA, Cooper CD, Roos AK, Aitkenhead H, Oppermann UC, Cho HJ, Osman R, Gileadi O
PLoS ONE. 2016 11(2):e0148762. doi: 10.1371/journal.pone.0148762
PMID: 26910052

Conformational Plasticity of the MAGE-A3 Protein as a Therapeutic Strategy in Multiple Myeloma
Osman, R; Cho, HJ; Mei, AH; Newman, JA; Gileadi, O;
Biophysical Journal. 2016 110:535A-535A. doi: 10.1016/j.bpj.2015.11.2862
PMID:

Identification of LACTB2, a metallo-β-lactamase protein, as a human mitochondrial endoribonuclease.
Levy S, Allerston CK, Liveanu V, Habib MR, Gileadi O, Schuster G
Nucleic Acids Res.. 2016 . doi: 10.1093/nar/gkw050
PMID: 26826708

EXD2 promotes homologous recombination by facilitating DNA end resection.
Broderick R, Nieminuszczy J, Baddock HT, Deshpande RA, Gileadi O, Paull TT, McHugh PJ, Niedzwiedz W
Nat. Cell Biol.. 2016 . doi: 10.1038/ncb3303
PMID: 26807646

2015

The structures of the SNM1A and SNM1B/Apollo nuclease domains reveal a potential basis for their distinct DNA processing activities.
Allerston CK, Lee SY, Newman JA, Schofield CJ, McHugh PJ, Gileadi O
Nucleic Acids Res.. 2015 . doi: 10.1093/nar/gkv1256
PMID: 26582912

Structural basis of metallo-β-lactamase inhibition by captopril stereoisomers.
Brem J, van Berkel SS, Zollman D, Lee SY, Gileadi O, McHugh PJ, Walsh TR, McDonough MA, Schofield CJ
Antimicrob. Agents Chemother.. 2015 . doi: 10.1128/AAC.01335-15
PMID: 26482303

CSB interacts with SNM1A and promotes DNA interstrand crosslink processing
Iyama, T; Lee, SY; Berquist, BR; Gileadi, O; Bohr, VA; Seidman, MM; McHugh, PJ; Wilson, DM;
Nucleic Acids Research. 2015 43:247-258. doi: 10.1093/nar/gku1279
PMID:

Crystal structure of the Bloom's syndrome helicase indicates a role for the HRDC domain in conformational changes.
Newman JA, Savitsky P, Allerston CK, Bizard AH, Özer Ö, Sarlós K, Liu Y, Pardon E, Steyaert J, Hickson ID, Gileadi O
Nucleic Acids Res.. 2015 . doi: 10.1093/nar/gkv373
PMID: 25901030

Human RECQ1 helicase-driven DNA unwinding, annealing, and branch migration: Insights from DNA complex structures.
Pike AC, Gomathinayagam S, Swuec P, Berti M, Zhang Y, Schnecke C, Marino F, von Delft F, Renault L, Costa A, Gileadi O, Vindigni A
Proc. Natl. Acad. Sci. U.S.A.. 2015 . doi: 10.1073/pnas.1417594112
PMID: 25831490

Structural insights into the autoregulation and cooperativity of the human transcription factor Ets-2.
Newman, JA; Cooper, CDO; Aitkenhead, H; Gileadi, O;
Journal of Biological Chemistry. 2015 290:8539-8549. doi: 10.1074/jbc.M114.619270
PMID: 25670864

2014

CSB interacts with SNM1A and promotes DNA interstrand crosslink processing.
Iyama T, Lee SY, Berquist BR, Gileadi O, Bohr VA, Seidman MM, McHugh PJ, Wilson DM
Nucleic Acids Res.. 2014 . doi: 10.1093/nar/gku1279
PMID: 25505141

Mechanistic insight into the interaction of BLM helicase with intra-strand G-quadruplex structures.
Chatterjee S, Zagelbaum J, Savitsky P, Sturzenegger A, Huttner D, Janscak P, Hickson ID, Gileadi O, Rothenberg E
Nat Commun. 2014 5:5556. doi: 10.1038/ncomms6556
PMID: 25418155

2013

Medium-throughput production of recombinant human proteins: protein production in insect cells.
Mahajan P, Strain-Damerell C, Gileadi O, Burgess-Brown NA
Methods Mol. Biol.. 2013 1091:95-121. doi: 10.1007/978-1-62703-691-7_6
PMID: 24203326

Medium-throughput production of recombinant human proteins: ligation-independent cloning.
Strain-Damerell C, Mahajan P, Gileadi O, Burgess-Brown NA
Methods Mol. Biol.. 2013 1091:55-72. doi: 10.1007/978-1-62703-691-7_4
PMID: 24203324

Synthesis and SAR studies of 5-(pyridin-4-yl)-1,3,4-thiadiazol-2-amine derivatives as potent inhibitors of Bloom helicase
Rosenthal, AS; Dexheimer, TS; Jadhav, A; Simeonov, A; Maloney, DJ; Gileadi, O; Nguyen, GH; Hickson, ID; Chu, WK;
Bioorganic and Medicinal Chemistry Letters. 2013 :-. doi: 10.1016/j.bmcl.2013.08.025
PMID: 24012121

Crystal Structures of Malonyl-Coenzyme A Decarboxylase Provide Insights into Its Catalytic Mechanism and Disease-Causing Mutations.
Froese DS, Forouhar F, Tran TH, Vollmar M, Kim YS, Lew S, Neely H, Seetharaman J, Shen Y, Xiao R, Acton TB, Everett JK, Cannone G, Puranik S, Savitsky P, Krojer T, Pilka ES, Kiyani W, Lee WH, Marsden BD, von Delft F, Allerston CK, Spagnolo L, Gileadi O, Montelione GT, Oppermann U, Yue WW, Tong L
Structure. 2013 . doi: 10.1016/j.str.2013.05.001
PMID: 23791943

Homozygous mutations in a predicted endonuclease are a novel cause of congenital dyserythropoietic anemia type I.
Babbs, C; Roberts, NA; Sanchez-Pulido, L; McGowan, SJ; Ahmed, MR; Brown, JM; Sabry, MA; WGS500 Consortium, ; Bentley, DR; McVean, GA; Donnelly, P; Gileadi, O; Ponting, CP; Higgs, DR; Buckle, VJ;
Haematologica. 2013 98:1383-1387. doi: 10.3324/haematol.2013.089490
PMID: 23716552

Structural Basis for Cul3 Protein Assembly with the BTB-Kelch Family of E3 Ubiquitin Ligases.
Canning P, Cooper CD, Krojer T, Murray JW, Pike AC, Chaikuad A, Keates T, Thangaratnarajah C, Hojzan V, Marsden BD, Gileadi O, Knapp S, von Delft F, Bullock AN
J. Biol. Chem.. 2013 288(11):7803-14. doi: 10.1074/jbc.M112.437996
PMID: 23349464

A small molecule inhibitor of the BLM helicase modulates chromosome stability in human cells.
Nguyen GH, Dexheimer TS, Rosenthal AS, Chu WK, Singh DK, Mosedale G, Bachrati CZ, Schultz L, Sakurai M, Savitsky P, Abu M, McHugh PJ, Bohr VA, Harris CC, Jadhav A, Gileadi O, Maloney DJ, Simeonov A, Hickson ID
Chem. Biol.. 2013 20(1):55-62. doi: 10.1016/j.chembiol.2012.10.016
PMID: 23352139

2012

Mutations in multidomain protein MEGF8 identify a Carpenter syndrome subtype associated with defective lateralization.
Twigg SR, Lloyd D, Jenkins D, Elçioglu NE, Cooper CD, Al-Sannaa N, Annagür A, Gillessen-Kaesbach G, Hüning I, Knight SJ, Goodship JA, Keavney BD, Beales PL, Gileadi O, McGowan SJ, Wilkie AO
Am. J. Hum. Genet.. 2012 91(5):897-905. doi: 10.1016/j.ajhg.2012.08.027
PMID: 23063620

Mutations in multidomain protein MEGF8 identify a carpenter syndrome subtype associated with defective lateralization
Twigg, SRF; Lloyd, D; Jenkins, D; Elçioglu, NE; Cooper, CDO; Al-Sannaa, N; Annagür, A; Gillessen-Kaesbach, G; Hüning, I; Knight, SJL; Goodship, JA; Keavney, BD; Beales, PL; Gileadi, O; McGowan, SJ; Wilkie, AOM;
American Journal of Human Genetics. 2012 91:897-905. doi: 10.1016/j.ajhg.2012.08.027
PMID:

A Small Molecule Inhibitor of the BLM Helicase That Modulates Chromosome Stability in Human Cells
Dexheimer, TS; Nguyen, GH; Rosenthal, AS; Bohr, VA; Harris, CC; Jadhav, A; Gileadi, O; Maloney, DJ; Simeonov, A; Hickson, ID;
European Journal of Cancer. 2012 48:85-85. doi: 10.1016/S0959-8049(12)72074-9
PMID:

The PHD and chromo domains regulate the ATPase activity of the human chromatin remodeler CHD4.
Watson AA, Mahajan P, Mertens HD, Deery MJ, Zhang W, Pham P, Du X, Bartke T, Zhang W, Edlich C, Berridge G, Chen Y, Burgess-Brown NA, Kouzarides T, Wiechens N, Owen-Hughes T, Svergun DI, Gileadi O, Laue ED
J. Mol. Biol.. 2012 422(1):3-17. doi: 10.1016/j.jmb.2012.04.031
PMID: 22575888

Characterization of the human SNM1A and SNM1B/Apollo DNA repair exonucleases.
Sengerová B, Allerston CK, Abu M, Lee SY, Hartley J, Kiakos K, Schofield CJ, Hartley JA, Gileadi O, McHugh PJ
J. Biol. Chem.. 2012 287(31):26254-67. doi: 10.1074/jbc.M112.367243
PMID: 22692201

2011

Human SNM1A and XPF-ERCC1 collaborate to initiate DNA interstrand cross-link repair.
Wang AT, Sengerová B, Cattell E, Inagawa T, Hartley JM, Kiakos K, Burgess-Brown NA, Swift LP, Enzlin JH, Schofield CJ, Gileadi O, Hartley JA, McHugh PJ
Genes Dev.. 2011 25(17):1859-70. doi: 10.1101/gad.15699211
PMID: 21896658

Human SNM1a and XPF-ERCC1 collaborate to initiate DNA interstrand cross-link repair
Wang, AT; Sengerová, B; Cattell, E; Inagawa, T; Hartley, JM; Kiakos, K; Burgess-Brown, NA; Swift, LP; Enzlin, JH; Schofield, CJ; Gileadi, O; Hartley, JA; McHugh, PJ;
Genes and Development. 2011 25:1859-1870. doi: 10.1101/gad.15699211
PMID:

Identification of macrodomain proteins as novel O-acetyl-ADP-ribose deacetylases.
Chen D, Vollmar M, Rossi MN, Phillips C, Kraehenbuehl R, Slade D, Mehrotra PV, von Delft F, Crosthwaite SK, Gileadi O, Denu JM, Ahel I
J. Biol. Chem.. 2011 286(15):13261-71. doi: 10.1074/jbc.M110.206771
PMID: 21257746

Structural basis of fumarate hydratase deficiency.
Picaud S, Kavanagh KL, Yue WW, Lee WH, Muller-Knapp S, Gileadi O, Sacchettini J, Oppermann U
J. Inherit. Metab. Dis.. 2011 34(3):671-6. doi: 10.1007/s10545-011-9294-8
PMID: 21445611

High-performance liquid chromatography separation and intact mass analysis of detergent-solubilized integral membrane proteins.
Berridge G, Chalk R, D'Avanzo N, Dong L, Doyle D, Kim JI, Xia X, Burgess-Brown N, Deriso A, Carpenter EP, Gileadi O
Anal. Biochem.. 2011 410(2):272-80. doi: 10.1016/j.ab.2010.11.008
PMID: 21093405

2010

High-performance liquid chromatography separation and intact mass analysis of detergent-solubilized integral membrane proteins
Berridge, G; Chalk, R; Burgess-Brown, NA; Carpenter, EP; Gileadi, O;
Analytical Biochemistry. 2010 :-. doi: 10.1016/j.ab.2010.11.008
PMID:

A prominent β-hairpin structure in the winged-helix domain of RECQ1 is required for DNA unwinding and oligomer formation.
Lucic B, Zhang Y, King O, Mendoza-Maldonado R, Berti M, Niesen FH, Burgess-Brown NA, Pike AC, Cooper CD, Gileadi O, Vindigni A
Nucleic Acids Res.. 2010 39(5):1703-17. doi: 10.1093/nar/gkq1031
PMID: 21059676

Isolated short CTG/CAG DNA slip-outs are repaired efficiently by hMutSbeta, but clustered slip-outs are poorly repaired.
Panigrahi GB, Slean MM, Simard JP, Gileadi O, Pearson CE
Proc. Natl. Acad. Sci. U.S.A.. 2010 107(28):12593-8. doi: 10.1073/pnas.0909087107
PMID: 20571119

High-throughput production of human proteins for crystallization: the SGC experience.
Savitsky P, Bray J, Cooper CD, Marsden BD, Mahajan P, Burgess-Brown NA, Gileadi O
J. Struct. Biol.. 2010 172(1):3-13. doi: 10.1016/j.jsb.2010.06.008
PMID: 20541610

Probing the structural basis of RecQ helicase function.
Vindigni A, Marino F, Gileadi O
Biophys. Chem.. 2010 149(3):67-77. doi: 10.1016/j.bpc.2010.03.012
PMID: 20392558

2009

Structure of the human RECQ1 helicase reveals a putative strand-separation pin.
Pike AC, Shrestha B, Popuri V, Burgess-Brown N, Muzzolini L, Costantini S, Vindigni A, Gileadi O
Proc. Natl. Acad. Sci. U.S.A.. 2009 106(4):1039-44. doi: 10.1073/pnas.0806908106
PMID: 19151156

2008

High throughput production of recombinant human proteins for crystallography.
Gileadi O, Burgess-Brown NA, Colebrook SM, Berridge G, Savitsky P, Smee CE, Loppnau P, Johansson C, Salah E, Pantic NH
Methods Mol. Biol.. 2008 426:221-46. doi: 10.1007/978-1-60327-058-8_14
PMID: 18542867

Codon optimization can improve expression of human genes in Escherichia coli: A multi-gene study.
Burgess-Brown NA, Sharma S, Sobott F, Loenarz C, Oppermann U, Gileadi O
Protein Expr. Purif.. 2008 59(1):94-102. doi: 10.1016/j.pep.2008.01.008
PMID: 18289875

Baculovirus expression vector system: An emerging host for high-throughput eukaryotic protein expression
Shrestha, B; Smee, C; Gileadi, O;
Methods in Molecular Biology. 2008 439:269-289. doi: 10.1007/978-1-59745-188-8_19
PMID:

2007

The scientific impact of the Structural Genomics Consortium: a protein family and ligand-centered approach to medically-relevant human proteins.
Gileadi O, Knapp S, Lee WH, Marsden BD, Müller S, Niesen FH, Kavanagh KL, Ball LJ, von Delft F, Doyle DA, Oppermann UC, Sundström M
J. Struct. Funct. Genomics. 2007 8(2-3):107-19. doi: 10.1007/s10969-007-9027-2
PMID: 17932789

Crystal structures of histone demethylase JMJD2A reveal basis for substrate specificity.
Ng SS, Kavanagh KL, McDonough MA, Butler D, Pilka ES, Lienard BM, Bray JE, Savitsky P, Gileadi O, von Delft F, Rose NR, Offer J, Scheinost JC, Borowski T, Sundstrom M, Schofield CJ, Oppermann U
Nature. 2007 448(7149):87-91. doi: 10.1038/nature05971
PMID: 17589501

2006

Direct observation of RuvAB-catalyzed branch migration of single Holliday junctions (vol 101, pg 11605, 2004)
Amit, R; Gileadi, O; Stavans, J;
Proceedings of the National Academy of Sciences. 2006 103:12654-12654. doi:
PMID:

2005

The structural genomics consortium: High-output structure determination
Gileadi, O; Colebrook, S; Burgess, N; Berridge, G; Haroniti, A; Shrestha, B; Smee, C; Sundstrom, M;
FASEB Journal. 2005 19:A315-A315. doi:
PMID:

2004

Direct observation of RuvAB-catalyzed branch migration of single Holliday junctions.
Amit, R; Gileadi, O; Stavans, J;
Proceedings of the National Academy of Sciences. 2004 101:11605-11610. doi: 10.1073/pnas.0404332101
PMID: 15292509

Electron microscopy of wet tissues: a case study in renal pathology.
Nyska, A; Cummings, CA; Vainshtein, A; Nadler, J; Ezov, N; Grunfeld, Y; Gileadi, O; Behar, V;
Toxicologic Pathology. 2004 32:357-363. doi: 10.1080/01926230490431772
PMID: 15204979

Fat cell precursors from different human fat depots are distinct: Morphological and gene expression profiles
Kirkland, J; Tchkonia, T; Frampton, G; Lenberg, M; Giorgadze, N; Sabban, A; Pirtskhalava, T; Cartwright, M; Gerry, N; Forse, A; Thomou, T; Gileadi, O;
International Journal of Obesity. 2004 28:S99-S99. doi:
PMID:

Scanning electron microscopy of cells and tissues under fully hydrated conditions.
Thiberge, S; Nechushtan, A; Sprinzak, D; Gileadi, O; Behar, V; Zik, O; Chowers, Y; Michaeli, S; Schlessinger, J; Moses, E;
Proceedings of the National Academy of Sciences. 2004 101:3346-3351. doi: 10.1073/pnas.0400088101
PMID: 14988502

A novel method for "Wet" SEM.
Barshack, I; Kopolovic, J; Chowers, Y; Gileadi, O; Vainshtein, A; Zik, O; Behar, V;
Ultrastructural Pathology. 2004 28:29-31. doi:
PMID: 14967596

2003

Squid sperm to clam eggs: imaging wet samples in a scanning electron microscope.
Gileadi, O; Sabban, A;
Biological Bulletin. 2003 205:177-179. doi: 10.2307/1543239
PMID: 14583516

Electron microscopy of wet cells and tissues
Gileadi, O; Behar, V; Ezer, S; Horowitz, M; Nechushtan, A; Sabban, A; Sprinzak, D; Vainshtein, A; Zik, O;
FASEB Journal. 2003 17:A1080-A1080. doi:
PMID:

Scanning electron microscopy of wet biological specimens
Gileadi, O; Behar, V; Ezer, S; Horowitz, M; Nechushtan, A; Sabban, A; Sprinzak, D; Vainshtein, A; Zik, O;
Microscopy and Microanalysis. 2003 9:486-487. doi:
PMID:

2002

Compaction of single DNA molecules by histone-like binding protein HU.
Schnurr, B; Amit, R; Oppenheim, AB; Gileadi, O; Stavans, J;
Biophysical Journal. 2002 82:51A-51A. doi:
PMID:

2001

Objective-type dark-field illumination for scattering from microbeads.
Braslavsky, I; Amit, R; Jaffar Ali, BM; Gileadi, O; Oppenheim, A; Stavans, J;
Applied Optics. 2001 40:5650-5657. doi:
PMID: 18364854

Compaction of single DNA molecules induced by binding of integration host factor (IHF).
Ali, BM; Amit, R; Braslavsky, I; Oppenheim, AB; Gileadi, O; Stavans, J;
Proceedings of the National Academy of Sciences. 2001 98:10658-10663. doi: 10.1073/pnas.181029198
PMID: 11535804

Involvement of yeast carboxy-terminal domain kinase I (CTDK-I) in transcription elongation in vivo.
Jona, G; Wittschieben, BO; Svejstrup, JQ; Gileadi, O;
Gene. 2001 267:31-36. doi:
PMID: 11311553

Autophosphorylation restrains the apoptotic activity of DRP-1 kinase by controlling dimerization and calmodulin binding.
Shani, G; Henis-Korenblit, S; Jona, G; Gileadi, O; Eisenstein, M; Ziv, T; Admon, A; Kimchi, A;
EMBO Journal. 2001 20:1099-1113. doi: 10.1093/emboj/20.5.1099
PMID: 11230133

2000

Distinct regions of MAT1 regulate cdk7 kinase and TFIIH transcription activities.
Busso, D; Keriel, A; Sandrock, B; Poterszman, A; Gileadi, O; Egly, JM;
Journal of Biological Chemistry. 2000 275:22815-22823. doi: 10.1074/jbc.M002578200
PMID: 10801852

Glucose starvation induces a drastic reduction in the rates of both transcription and degradation of mRNA in yeast.
Jona, G; Choder, M; Gileadi, O;
BBA - Biochimica et Biophysica Acta. 2000 1491:37-48. doi:
PMID: 10760568

Subunit interactions in yeast transcription/repair factor TFIIH. Requirement for Tfb3 subunit in nucleotide excision repair.
Feaver, WJ; Huang, W; Gileadi, O; Myers, L; Gustafsson, CM; Kornberg, RD; Friedberg, EC;
Journal of Biological Chemistry. 2000 275:5941-5946. doi:
PMID: 10681587

Erratum: Cak1 is required for Kin28 phosphorylation and activation in vivo (Molecular and Cellular Biology (1999) 19:12 (6365-6373))
Espinoza, FH; Farrell, A; Nourse, JL; Chamberlin, HM; Gileadi, O; Morgan, DO;
Molecular and Cellular Biology. 2000 20:1898-. doi: 10.1128/MCB.20.5.1898-1898.2000
PMID:

1999

An unusual eukaryotic protein phosphatase required for transcription by RNA polymerase II and CTD dephosphorylation in S. cerevisiae.
Kobor, MS; Archambault, J; Lester, W; Holstege, FC; Gileadi, O; Jansma, DB; Jennings, EG; Kouyoumdjian, F; Davidson, AR; Young, RA; Greenblatt, J;
Molecular Cell. 1999 4:55-62. doi:
PMID: 10445027

1998

Cak1 is required for Kin28 phosphorylation and activation in vivo.
Espinoza, FH; Farrell, A; Nourse, JL; Chamberlin, HM; Gileadi, O; Morgan, DO;
Molecular and Cellular Biology. 1998 18:6365-6373. doi:
PMID: 9774652

Analysis of the Ac promoter: structure and regulation.
Fridlender, M; Sitrit, Y; Shaul, O; Gileadi, O; Levy, AA;
Molecular and General Genetics MGG. 1998 258:306-314. doi:
PMID: 9645437

1995

Partial truncation of the yeast RNA polymerase II carboxyl-terminal domain preferentially reduces expression of glycolytic genes.
Meisels, E; Gileadi, O; Corden, JL;
Journal of Biological Chemistry. 1995 270:31255-31261. doi:
PMID: 8537392

1994

Yeast TFIIE. Cloning, expression, and homology to vertebrate proteins.
Feaver, WJ; Henry, NL; Bushnell, DA; Sayre, MH; Brickner, JH; Gileadi, O; Kornberg, RD;
Journal of Biological Chemistry. 1994 269:27549-27553. doi:
PMID: 7961670

1993

CHARACTERIZATION OF YEAST RNA POLYMERASE-II TRANSCRIPTION FACTOR-B AND CTD KINASE
FEAVER, WJ; GILEADI, O; KORNBERG, RD;
Journal of Cellular Biochemistry. 1993 :71-71. doi:
PMID:

YEAST RNA POLYMERASE-II TRANSCRIPTION - STRUCTURE, MECHANISM AND REGULATION
KORNBERG, RD; CHASMAN, DI; DARST, SA; DAVID, P; EDWARDS, AM; FEAVER, WJ; FLANAGAN, PM; GILEADI, O; HENRY, NL; KELLEHER, RJ; LI, Y; LORCH, Y; MEREDITH, G; POGLITSCH, C; SAYRE, MH; TSCHOCHNER, H;
Journal of Cellular Biochemistry. 1993 :55-55. doi:
PMID:

1992

Cloning of a subunit of yeast RNA polymerase II transcription factor b and CTD kinase.
Gileadi, O; Feaver, WJ; Kornberg, RD;
Science. 1992 257:1389-1392. doi:
PMID: 1445600

Transcription of the H19 gene in differentiating cytotrophoblasts from human placenta.
Rachmilewitz, J; Gileadi, O; Eldar-Geva, T; Schneider, T; de-Groot, N; Hochberg, A;
Molecular Reproduction and Development. 1992 32:196-202. doi: 10.1002/mrd.1080320303
PMID: 1497869

YEAST RNA POLYMERASE-II TRANSCRIPTION - ENZYMATIC-ACTIVITIES OF GENERAL INITIATION-FACTORS, 3-D STRUCTURES OF INITIATION-FACTORS AND RNA-POLYMERASE, AND MECHANISM OF TRANSCRIPTIONAL ACTIVATION
KORNBERG, R; CHASMAN, D; DARST, S; EDWARDS, A; FEAVER, W; FLANAGAN, P; GILEADI, O; HINDS, D; KELLEHER, R; MEREDITH, G; LI, Y; LORCH, Y; PARK, B; SAYRE, M; TSCHOCHNER, H;
FASEB Journal. 1992 6:A274-A274. doi:
PMID:

1991

CTD kinase associated with yeast RNA polymerase II initiation factor b.
Feaver, WJ; Gileadi, O; Li, Y; Kornberg, RD;
Cell. 1991 67:1223-1230. doi:
PMID: 1836979

Purification and characterization of yeast RNA polymerase II transcription factor b.
Feaver, WJ; Gileadi, O; Kornberg, RD;
Journal of Biological Chemistry. 1991 266:19000-19005. doi:
PMID: 1918015

1988

THE EXPRESSION OF PROTOONCOGENES AND 2'-5' OLIGOADENYLATE SYNTHETASE IN DIFFERENTIATING HUMAN TROPHOBLASTIC CELLS-INVITRO
GILEADI, O; SCHNEIDER, T; CHEBATH, J; DEGROOT, N; HOCHBERG, AA;
. 1988 798:251-260. doi:
PMID:

1984

Location of RNase and RNase inhibitor on free cytoplasmic mRNA-protein particles from human placenta.
Gileadi, O; Lorberboum, H; de Groot, N; Hochberg, AA;
Molecular Biology Reports. 1984 9:241-244. doi:
PMID: 6708950

1982

Phosphorylation of proteins in cultured human placenta
Galski, H; Weinstein, D; Gileadi, O;
Biochemistry International. 1982 5:137-143. doi:
PMID:

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