The SGC employs a combination of off-the-shelf and custom-developed technology and processes. In the Toronto labs, our capabilities include:
High throughput cloning:
- LEX - Large Scale Bubbling System for Bacterial Cultures
- Baculovirus expression
- EX2 expression optimization system
- High throughput crystallization (including SGC custom screens)
- High intensity diffractometer
More information is available here: click to download
Histone recognition and large-scale structural analysis of the human bromodomain family. Cell. 2012 Mar 30;149(1):214-31.
Insights into protein kinase regulation and inhibition by large scale structural comparison. Biochim Biophys Acta. 2010 Mar;1804(3):429-32
Large-scale structural analysis of the classical human protein tyrosine phosphatome. Cell. 2009 Jan 23;136(2):352-63
Structural and biochemical characterization of the human cyclophilin family of peptidyl-prolyl isomerases. PLoS Biol. 2010 Jul 27;8(7):e1000439.
Structural basis for protein-protein interactions in the 14-3-3 protein family. Proc Natl Acad Sci U S A. 2006 Nov 14;103(46):17237-42
Large-scale structural biology of the human proteome. Annu Rev Biochem. 2009;78:541-68
The scientific impact of the Structural Genomics Consortium: a protein family and ligand-centered approach to medically-relevant human proteins. J Struct Funct Genomics. 2007 Sep;8(2-3):107-19