Opher Gileadi

Principal Investigator

University of Oxford
+44 (0)1865 617572

Opher Gileadi studied at the Hebrew University. He did his post-doc at Stanford, then led a research group at the Weizmann Institute. Opher has studied the biochemical basis of gene expression and of DNA repair, using a wide range of approaches including differential mRNA analysis in human trophoblast cells, studies of transcription factors using purified systems and yeast genetics, and single-molecule analyses of protein:DNA interactions.

Research Areas

At the SGC, Opher established the Biotechnology group, developing methods for high-throughput production of human proteins for crystallization. He now heads the Genome Integrity group, which studies DNA repair proteins and on proteins associated with human genetic disease. Recent work includes structures of RecQ DNA helicases and DNA cross-link repair endonucleases, and biochemical characterization of genes mutated in Carpenter’s syndrome and congenital dyserythropoietic anemia. We are also developing methods to intervene with DNA repair using small molecules such as the new inhibitors of the Bloom DNA helicase.

Group Members

  • Joseph A Newman
  • Hazel Aitkenhead

Publications

Structures of Two Melanoma-Associated Antigens Suggest Allosteric Regulation of Effector Binding.
Newman, JA; Cooper, CD; Roos, AK; Aitkenhead, H; Oppermann, UC; Cho, HJ; Osman, R; Gileadi, O;
PLoS One. 2016 11:e0148762-. doi:
PMID: 26910052

Identification of LACTB2, a metallo-β-lactamase protein, as a human mitochondrial endoribonuclease.
Levy, S; Allerston, CK; Liveanu, V; Habib, MR; Gileadi, O; Schuster, G;
Nucleic Acids Research. 2016 44:1813-1832. doi:
PMID: 26826708

Crystal structure of the Bloom's syndrome helicase indicates a role for the HRDC domain in conformational changes.
Newman, JA; Savitsky, P; Allerston, CK; Bizard, AH; Özer, Ö; Sarlós, K; Liu, Y; Pardon, E; Steyaert, J; Hickson, ID; Gileadi, O;
Nucleic Acids Research. 2015 43:5221-5235. doi:
PMID: 25901030

Structures of the Ets Protein DNA-binding Domains of Transcription Factors Etv1, Etv4, Etv5, and Fev: DETERMINANTS OF DNA BINDING AND REDOX REGULATION BY DISULFIDE BOND FORMATION.
Cooper, CD; Newman, JA; Aitkenhead, H; Allerston, CK; Gileadi, O;
Journal of Biological Chemistry. 2015 290:13692-13709. doi:
PMID: 25866208

Structure of the Helicase Domain of DNA Polymerase Theta Reveals a Possible Role in the Microhomology-Mediated End-Joining Pathway.
Newman, JA; Cooper, CD; Aitkenhead, H; Gileadi, O;
Structure. 2015 23:2319-2330. doi:
PMID: 26636256

Human RECQ1 helicase-driven DNA unwinding, annealing, and branch migration: insights from DNA complex structures.
Pike, AC; Gomathinayagam, S; Swuec, P; Berti, M; Zhang, Y; Schnecke, C; Marino, F; von Delft, F; Renault, L; Costa, A; Gileadi, O; Vindigni, A;
Proceedings of the National Academy of Sciences of USA. 2015 112:4286-4291. doi:
PMID: 25831490

The structures of the SNM1A and SNM1B/Apollo nuclease domains reveal a potential basis for their distinct DNA processing activities.
Allerston, CK; Lee, SY; Newman, JA; Schofield, CJ; McHugh, PJ; Gileadi, O;
Nucleic Acids Research. 2015 43:11047-11060. doi:
PMID: 26582912

Medium-throughput production of recombinant human proteins: ligation-independent cloning.
Strain-Damerell, C; Mahajan, P; Gileadi, O; Burgess-Brown, NA;
Methods in Molecular Biology. 2014 1091:55-72. doi: 10.1007/978-1-62703-691-7_4
PMID: 24203324

Medium-throughput production of recombinant human proteins: protein production in insect cells.
Mahajan, P; Strain-Damerell, C; Gileadi, O; Burgess-Brown, NA;
Methods in Molecular Biology. 2014 1091:95-121. doi: 10.1007/978-1-62703-691-7_6
PMID: 24203326

Crystal structures of malonyl-coenzyme A decarboxylase provide insights into its catalytic mechanism and disease-causing mutations.
Froese, DS; Forouhar, F; Tran, TH; Vollmar, M; Kim, YS; Lew, S; Neely, H; Seetharaman, J; Shen, Y; Xiao, R; Acton, TB; Everett, JK; Cannone, G; Puranik, S; Savitsky, P; Krojer, T; Pilka, ES; Kiyani, W; Lee, WH; Marsden, BD; von Delft, F; Allerston, CK; Spagnolo, L; Gileadi, O; Montelione, GT; Oppermann, U; Yue, WW; Tong, L;
Structure. 2013 21:1182-1192. doi: 10.1016/j.str.2013.05.001
PMID: 23791943

Homozygous mutations in a predicted endonuclease are a novel cause of congenital dyserythropoietic anemia type I.
Babbs, C; Roberts, NA; Sanchez-Pulido, L; McGowan, SJ; Ahmed, MR; Brown, JM; Sabry, MA; WGS500 Consortium, ; Bentley, DR; McVean, GA; Donnelly, P; Gileadi, O; Ponting, CP; Higgs, DR; Buckle, VJ;
Haematologica: the hematology journal. 2013 98:1383-1387. doi: 10.3324/haematol.2013.089490
PMID: 23716552

A small molecule inhibitor of the BLM helicase modulates chromosome stability in human cells.
Nguyen, GH; Dexheimer, TS; Rosenthal, AS; Chu, WK; Singh, DK; Mosedale, G; Bachrati, CZ; Schultz, L; Sakurai, M; Savitsky, P; Abu, M; McHugh, PJ; Bohr, VA; Harris, CC; Jadhav, A; Gileadi, O; Maloney, DJ; Simeonov, A; Hickson, ID;
Chemistry and Biology. 2013 20:55-62. doi: 10.1016/j.chembiol.2012.10.016
PMID: 23352139

Characterization of the human SNM1A and SNM1B/Apollo DNA repair exonucleases.
Sengerová, B; Allerston, CK; Abu, M; Lee, SY; Hartley, J; Kiakos, K; Schofield, CJ; Hartley, JA; Gileadi, O; McHugh, PJ;
Journal of Biological Chemistry. 2012 287:26254-26267. doi: 10.1074/jbc.M112.367243
PMID: 22692201

The PHD and chromo domains regulate the ATPase activity of the human chromatin remodeler CHD4.
Watson, AA; Mahajan, P; Mertens, HD; Deery, MJ; Zhang, W; Pham, P; Du, X; Bartke, T; Zhang, W; Edlich, C; Berridge, G; Chen, Y; Burgess-Brown, NA; Kouzarides, T; Wiechens, N; Owen-Hughes, T; Svergun, DI; Gileadi, O; Laue, ED;
Journal of Molecular Biology. 2012 422:3-17. doi: 10.1016/j.jmb.2012.04.031
PMID: 22575888

Identification of macrodomain proteins as novel O-acetyl-ADP-ribose deacetylases.
Chen, D; Vollmar, M; Rossi, MN; Phillips, C; Kraehenbuehl, R; Slade, D; Mehrotra, PV; von Delft, F; Crosthwaite, SK; Gileadi, O; Denu, JM; Ahel, I;
Journal of Biological Chemistry. 2011 286:13261-13271. doi: 10.1074/jbc.M110.206771
PMID: 21257746

Human SNM1a and XPF-ERCC1 collaborate to initiate DNA interstrand cross-link repair
Wang, AT; Sengerová, B; Cattell, E; Inagawa, T; Hartley, JM; Kiakos, K; Burgess-Brown, NA; Swift, LP; Enzlin, JH; Schofield, CJ; Gileadi, O; Hartley, JA; McHugh, PJ;
Genes and Development. 2011 25:1859-1870. doi: 10.1101/gad.15699211
PMID:

High-performance liquid chromatography separation and intact mass analysis of detergent-solubilized integral membrane proteins.
Berridge, G; Chalk, R; D'Avanzo, N; Dong, L; Doyle, D; Kim, JI; Xia, X; Burgess-Brown, N; Deriso, A; Carpenter, EP; Gileadi, O;
Analytical Biochemistry. 2011 410:272-280. doi: 10.1016/j.ab.2010.11.008
PMID: 21093405

A prominent β-hairpin structure in the winged-helix domain of RECQ1 is required for DNA unwinding and oligomer formation.
Lucic, B; Zhang, Y; King, O; Mendoza-Maldonado, R; Berti, M; Niesen, FH; Burgess-Brown, NA; Pike, AC; Cooper, CD; Gileadi, O; Vindigni, A;
Nucleic Acids Research. 2011 39:1703-1717. doi: 10.1093/nar/gkq1031
PMID: 21059676

High-throughput production of human proteins for crystallization: the SGC experience.
Savitsky, P; Bray, J; Cooper, CD; Marsden, BD; Mahajan, P; Burgess-Brown, NA; Gileadi, O;
Journal of Structural Biology. 2010 172:3-13. doi: 10.1016/j.jsb.2010.06.008
PMID: 20541610

Probing the structural basis of RecQ helicase function.
Vindigni, A; Marino, F; Gileadi, O;
Biophysical Chemistry. 2010 149:67-77. doi: 10.1016/j.bpc.2010.03.012
PMID: 20392558

Structure of the human RECQ1 helicase reveals a putative strand-separation pin.
Pike, AC; Shrestha, B; Popuri, V; Burgess-Brown, N; Muzzolini, L; Costantini, S; Vindigni, A; Gileadi, O;
Proceedings of the National Academy of Sciences of USA. 2009 106:1039-1044. doi: 10.1073/pnas.0806908106
PMID: 19151156

Codon optimization can improve expression of human genes in Escherichia coli: A multi-gene study.
Burgess-Brown, NA; Sharma, S; Sobott, F; Loenarz, C; Oppermann, U; Gileadi, O;
Protein Expression and Purification. 2008 59:94-102. doi: 10.1016/j.pep.2008.01.008
PMID: 18289875

Direct observation of RuvAB-catalyzed branch migration of single Holliday junctions (vol 101, pg 11605, 2004)
Amit, R; Gileadi, O; Stavans, J;
Proceedings of the National Academy of Sciences of USA. 2006 103:12654-12654. doi:
PMID:

Scanning electron microscopy of cells and tissues under fully hydrated conditions.
Thiberge, S; Nechushtan, A; Sprinzak, D; Gileadi, O; Behar, V; Zik, O; Chowers, Y; Michaeli, S; Schlessinger, J; Moses, E;
Proceedings of the National Academy of Sciences of USA. 2004 101:3346-3351. doi: 10.1073/pnas.0400088101
PMID: 14988502

Direct observation of RuvAB-catalyzed branch migration of single Holliday junctions.
Amit, R; Gileadi, O; Stavans, J;
Proceedings of the National Academy of Sciences of USA. 2004 101:11605-11610. doi: 10.1073/pnas.0404332101
PMID: 15292509

Squid sperm to clam eggs: imaging wet samples in a scanning electron microscope.
Gileadi, O; Sabban, A;
Biological Bulletin. 2003 205:177-179. doi: 10.2307/1543239
PMID: 14583516

Compaction of single DNA molecules by histone-like binding protein HU.
Schnurr, B; Amit, R; Oppenheim, AB; Gileadi, O; Stavans, J;
Biophysical Journal. 2002 82:51A-51A. doi:
PMID:

Compaction of single DNA molecules induced by binding of integration host factor (IHF).
Ali, BM; Amit, R; Braslavsky, I; Oppenheim, AB; Gileadi, O; Stavans, J;
Proceedings of the National Academy of Sciences of USA. 2001 98:10658-10663. doi: 10.1073/pnas.181029198
PMID: 11535804

2016

Metalloprotease SPRTN/DVC1 Orchestrates Replication-Coupled DNA-Protein Crosslink Repair.
Vaz, B; Popovic, M; Newman, JA; Fielden, J; Aitkenhead, H; Halder, S; Singh, AN; Vendrell, I; Fischer, R; Torrecilla, I; Drobnitzky, N; Freire, R; Amor, DJ; Lockhart, PJ; Kessler, BM; McKenna, GW; Gileadi, O; Ramadan, K;
Molecular Cell. 2016 64:704-719. doi: 10.1016/j.molcel.2016.09.032
PMID: 27871366

Identification of LACTB2, a metallo-β-lactamase protein, as a human mitochondrial endoribonuclease.
Levy, S; Allerston, CK; Liveanu, V; Habib, MR; Gileadi, O; Schuster, G;
Nucleic Acids Research. 2016 44:1813-1832. doi:
PMID: 26826708

EXD2 promotes homologous recombination by facilitating DNA end resection.
Broderick, R; Nieminuszczy, J; Baddock, HT; Deshpande, RA; Gileadi, O; Paull, TT; McHugh, PJ; Niedzwiedz, W;
Nature Cell Biology. 2016 18:271-280. doi:
PMID: 26807646

Structures of Two Melanoma-Associated Antigens Suggest Allosteric Regulation of Effector Binding.
Newman, JA; Cooper, CD; Roos, AK; Aitkenhead, H; Oppermann, UC; Cho, HJ; Osman, R; Gileadi, O;
PLoS One. 2016 11:e0148762-. doi:
PMID: 26910052

Cephalosporins inhibit human metallo β-lactamase fold DNA repair nucleases SNM1A and SNM1B/apollo.
Lee, SY; Brem, J; Pettinati, I; Claridge, TD; Gileadi, O; Schofield, CJ; McHugh, PJ;
Chemical Communications. 2016 52:6727-6730. doi:
PMID: 27121860

Structural Basis of Metallo-β-Lactamase Inhibition by Captopril Stereoisomers.
Brem, J; van Berkel, SS; Zollman, D; Lee, SY; Gileadi, O; McHugh, PJ; Walsh, TR; McDonough, MA; Schofield, CJ;
Antimicrobial Agents and Chemotherapy. 2016 60:142-150. doi:
PMID: 26482303

2015

Structure of the Helicase Domain of DNA Polymerase Theta Reveals a Possible Role in the Microhomology-Mediated End-Joining Pathway.
Newman, JA; Cooper, CD; Aitkenhead, H; Gileadi, O;
Structure. 2015 23:2319-2330. doi:
PMID: 26636256

The structures of the SNM1A and SNM1B/Apollo nuclease domains reveal a potential basis for their distinct DNA processing activities.
Allerston, CK; Lee, SY; Newman, JA; Schofield, CJ; McHugh, PJ; Gileadi, O;
Nucleic Acids Research. 2015 43:11047-11060. doi:
PMID: 26582912

CSB interacts with SNM1A and promotes DNA interstrand crosslink processing
Iyama, T; Lee, SY; Berquist, BR; Gileadi, O; Bohr, VA; Seidman, MM; McHugh, PJ; Wilson, DM;
Nucleic Acids Research. 2015 43:247-258. doi: 10.1093/nar/gku1279
PMID:

Crystal structure of the Bloom's syndrome helicase indicates a role for the HRDC domain in conformational changes.
Newman, JA; Savitsky, P; Allerston, CK; Bizard, AH; Özer, Ö; Sarlós, K; Liu, Y; Pardon, E; Steyaert, J; Hickson, ID; Gileadi, O;
Nucleic Acids Research. 2015 43:5221-5235. doi:
PMID: 25901030

Structures of the Ets Protein DNA-binding Domains of Transcription Factors Etv1, Etv4, Etv5, and Fev: DETERMINANTS OF DNA BINDING AND REDOX REGULATION BY DISULFIDE BOND FORMATION.
Cooper, CD; Newman, JA; Aitkenhead, H; Allerston, CK; Gileadi, O;
Journal of Biological Chemistry. 2015 290:13692-13709. doi:
PMID: 25866208

Human RECQ1 helicase-driven DNA unwinding, annealing, and branch migration: insights from DNA complex structures.
Pike, AC; Gomathinayagam, S; Swuec, P; Berti, M; Zhang, Y; Schnecke, C; Marino, F; von Delft, F; Renault, L; Costa, A; Gileadi, O; Vindigni, A;
Proceedings of the National Academy of Sciences of USA. 2015 112:4286-4291. doi:
PMID: 25831490

Structural insights into the autoregulation and cooperativity of the human transcription factor Ets-2.
Newman, JA; Cooper, CD; Aitkenhead, H; Gileadi, O;
Journal of Biological Chemistry. 2015 290:8539-8549. doi:
PMID: 25670864

CSB interacts with SNM1A and promotes DNA interstrand crosslink processing.
Iyama, T; Lee, SY; Berquist, BR; Gileadi, O; Bohr, VA; Seidman, MM; McHugh, PJ; Wilson, DM;
Nucleic Acids Research. 2015 43:247-258. doi:
PMID: 25505141

2014

Structures of soluble guanylate cyclase: implications for regulatory mechanisms and drug development.
G, O; i, ; l, ; e, ; a, ; d, ; i, ;
Biochemical Society Transactions. 2014 42:108-113. doi: 10.1042/BST20130228
PMID: 24450636

Recent advances in the structural molecular biology of Ets transcription factors: interactions, interfaces and inhibition.
Cooper, CD; Newman, JA; Gileadi, O;
Biochemical Society Transactions. 2014 42:130-138. doi: 10.1042/BST20130227
PMID: 24450640

Mechanistic insight into the interaction of BLM helicase with intra-strand G-quadruplex structures.
Chatterjee, S; Zagelbaum, J; Savitsky, P; Sturzenegger, A; Huttner, D; Janscak, P; Hickson, ID; Gileadi, O; Rothenberg, E;
Nature Communications. 2014 5:5556-. doi:
PMID: 25418155

Medium-throughput production of recombinant human proteins: ligation-independent cloning.
Strain-Damerell, C; Mahajan, P; Gileadi, O; Burgess-Brown, NA;
Methods in Molecular Biology. 2014 1091:55-72. doi: 10.1007/978-1-62703-691-7_4
PMID: 24203324

Medium-throughput production of recombinant human proteins: protein production in insect cells.
Mahajan, P; Strain-Damerell, C; Gileadi, O; Burgess-Brown, NA;
Methods in Molecular Biology. 2014 1091:95-121. doi: 10.1007/978-1-62703-691-7_6
PMID: 24203326

2013

Crystal structures of malonyl-coenzyme A decarboxylase provide insights into its catalytic mechanism and disease-causing mutations.
Froese, DS; Forouhar, F; Tran, TH; Vollmar, M; Kim, YS; Lew, S; Neely, H; Seetharaman, J; Shen, Y; Xiao, R; Acton, TB; Everett, JK; Cannone, G; Puranik, S; Savitsky, P; Krojer, T; Pilka, ES; Kiyani, W; Lee, WH; Marsden, BD; von Delft, F; Allerston, CK; Spagnolo, L; Gileadi, O; Montelione, GT; Oppermann, U; Yue, WW; Tong, L;
Structure. 2013 21:1182-1192. doi: 10.1016/j.str.2013.05.001
PMID: 23791943

Homozygous mutations in a predicted endonuclease are a novel cause of congenital dyserythropoietic anemia type I.
Babbs, C; Roberts, NA; Sanchez-Pulido, L; McGowan, SJ; Ahmed, MR; Brown, JM; Sabry, MA; WGS500 Consortium, ; Bentley, DR; McVean, GA; Donnelly, P; Gileadi, O; Ponting, CP; Higgs, DR; Buckle, VJ;
Haematologica: the hematology journal. 2013 98:1383-1387. doi: 10.3324/haematol.2013.089490
PMID: 23716552

Structural basis for Cul3 protein assembly with the BTB-Kelch family of E3 ubiquitin ligases.
Canning, P; Cooper, CD; Krojer, T; Murray, JW; Pike, AC; Chaikuad, A; Keates, T; Thangaratnarajah, C; Hojzan, V; Ayinampudi, V; Marsden, BD; Gileadi, O; Knapp, S; von Delft, F; Bullock, AN;
Journal of Biological Chemistry. 2013 288:7803-7814. doi: 10.1074/jbc.M112.437996
PMID: 23349464

A small molecule inhibitor of the BLM helicase modulates chromosome stability in human cells.
Nguyen, GH; Dexheimer, TS; Rosenthal, AS; Chu, WK; Singh, DK; Mosedale, G; Bachrati, CZ; Schultz, L; Sakurai, M; Savitsky, P; Abu, M; McHugh, PJ; Bohr, VA; Harris, CC; Jadhav, A; Gileadi, O; Maloney, DJ; Simeonov, A; Hickson, ID;
Chemistry and Biology. 2013 20:55-62. doi: 10.1016/j.chembiol.2012.10.016
PMID: 23352139

Synthesis and SAR studies of 5-(pyridin-4-yl)-1,3,4-thiadiazol-2-amine derivatives as potent inhibitors of Bloom helicase
Rosenthal, AS; Dexheimer, TS; Jadhav, A; Simeonov, A; Maloney, DJ; Gileadi, O; Nguyen, GH; Hickson, ID; Chu, WK;
Bioorganic and Medicinal Chemistry Letters. 2013 :-. doi: 10.1016/j.bmcl.2013.08.025
PMID: 24012121

2012

Mutations in multidomain protein MEGF8 identify a Carpenter syndrome subtype associated with defective lateralization.
Twigg, SR; Lloyd, D; Jenkins, D; Elçioglu, NE; Cooper, CD; Al-Sannaa, N; Annagür, A; Gillessen-Kaesbach, G; Hüning, I; Knight, SJ; Goodship, JA; Keavney, BD; Beales, PL; Gileadi, O; McGowan, SJ; Wilkie, AO;
American Journal of Human Genetics. 2012 91:897-905. doi: 10.1016/j.ajhg.2012.08.027
PMID: 23063620

Characterization of the human SNM1A and SNM1B/Apollo DNA repair exonucleases.
Sengerová, B; Allerston, CK; Abu, M; Lee, SY; Hartley, J; Kiakos, K; Schofield, CJ; Hartley, JA; Gileadi, O; McHugh, PJ;
Journal of Biological Chemistry. 2012 287:26254-26267. doi: 10.1074/jbc.M112.367243
PMID: 22692201

The PHD and chromo domains regulate the ATPase activity of the human chromatin remodeler CHD4.
Watson, AA; Mahajan, P; Mertens, HD; Deery, MJ; Zhang, W; Pham, P; Du, X; Bartke, T; Zhang, W; Edlich, C; Berridge, G; Chen, Y; Burgess-Brown, NA; Kouzarides, T; Wiechens, N; Owen-Hughes, T; Svergun, DI; Gileadi, O; Laue, ED;
Journal of Molecular Biology. 2012 422:3-17. doi: 10.1016/j.jmb.2012.04.031
PMID: 22575888

Structural and functional insights into the DNA replication factor Cdc45 reveal an evolutionary relationship to the DHH family of phosphoesterases.
Krastanova, I; Sannino, V; Amenitsch, H; Gileadi, O; Pisani, FM; Onesti, S;
Journal of Biological Chemistry. 2012 287:4121-4128. doi: 10.1074/jbc.M111.285395
PMID: 22147708

2011

Structural basis of fumarate hydratase deficiency.
Picaud, S; Kavanagh, KL; Yue, WW; Lee, WH; Muller-Knapp, S; Gileadi, O; Sacchettini, J; Oppermann, U;
Journal of Inherited Metabolic Disease. 2011 34:671-676. doi: 10.1007/s10545-011-9294-8
PMID: 21445611

Identification of macrodomain proteins as novel O-acetyl-ADP-ribose deacetylases.
Chen, D; Vollmar, M; Rossi, MN; Phillips, C; Kraehenbuehl, R; Slade, D; Mehrotra, PV; von Delft, F; Crosthwaite, SK; Gileadi, O; Denu, JM; Ahel, I;
Journal of Biological Chemistry. 2011 286:13261-13271. doi: 10.1074/jbc.M110.206771
PMID: 21257746

Human SNM1a and XPF-ERCC1 collaborate to initiate DNA interstrand cross-link repair
Wang, AT; Sengerová, B; Cattell, E; Inagawa, T; Hartley, JM; Kiakos, K; Burgess-Brown, NA; Swift, LP; Enzlin, JH; Schofield, CJ; Gileadi, O; Hartley, JA; McHugh, PJ;
Genes and Development. 2011 25:1859-1870. doi: 10.1101/gad.15699211
PMID:

High-performance liquid chromatography separation and intact mass analysis of detergent-solubilized integral membrane proteins.
Berridge, G; Chalk, R; D'Avanzo, N; Dong, L; Doyle, D; Kim, JI; Xia, X; Burgess-Brown, N; Deriso, A; Carpenter, EP; Gileadi, O;
Analytical Biochemistry. 2011 410:272-280. doi: 10.1016/j.ab.2010.11.008
PMID: 21093405

A prominent β-hairpin structure in the winged-helix domain of RECQ1 is required for DNA unwinding and oligomer formation.
Lucic, B; Zhang, Y; King, O; Mendoza-Maldonado, R; Berti, M; Niesen, FH; Burgess-Brown, NA; Pike, AC; Cooper, CD; Gileadi, O; Vindigni, A;
Nucleic Acids Research. 2011 39:1703-1717. doi: 10.1093/nar/gkq1031
PMID: 21059676

2010

Isolated short CTG/CAG DNA slip-outs are repaired efficiently by hMutSbeta, but clustered slip-outs are poorly repaired.
Panigrahi, GB; Slean, MM; Simard, JP; Gileadi, O; Pearson, CE;
Proceedings of the National Academy of Sciences of USA. 2010 107:12593-12598. doi: 10.1073/pnas.0909087107
PMID: 20571119

High-throughput production of human proteins for crystallization: the SGC experience.
Savitsky, P; Bray, J; Cooper, CD; Marsden, BD; Mahajan, P; Burgess-Brown, NA; Gileadi, O;
Journal of Structural Biology. 2010 172:3-13. doi: 10.1016/j.jsb.2010.06.008
PMID: 20541610

Probing the structural basis of RecQ helicase function.
Vindigni, A; Marino, F; Gileadi, O;
Biophysical Chemistry. 2010 149:67-77. doi: 10.1016/j.bpc.2010.03.012
PMID: 20392558

2009

Structure of the human RECQ1 helicase reveals a putative strand-separation pin.
Pike, AC; Shrestha, B; Popuri, V; Burgess-Brown, N; Muzzolini, L; Costantini, S; Vindigni, A; Gileadi, O;
Proceedings of the National Academy of Sciences of USA. 2009 106:1039-1044. doi: 10.1073/pnas.0806908106
PMID: 19151156

2008

Codon optimization can improve expression of human genes in Escherichia coli: A multi-gene study.
Burgess-Brown, NA; Sharma, S; Sobott, F; Loenarz, C; Oppermann, U; Gileadi, O;
Protein Expression and Purification. 2008 59:94-102. doi: 10.1016/j.pep.2008.01.008
PMID: 18289875

Baculovirus expression vector system: An emerging host for high-throughput eukaryotic protein expression
Shrestha, B; Smee, C; Gileadi, O;
Methods in Molecular Biology. 2008 439:269-289. doi: 10.1007/978-1-59745-188-8_19
PMID:

Baculovirus expression vector system: an emerging host for high-throughput eukaryotic protein expression.
Shrestha, B; Smee, C; Gileadi, O;
Methods in Molecular Biology. 2008 439:269-289. doi: 10.1007/978-1-59745-188-8_19
PMID: 18370110

High throughput production of recombinant human proteins for crystallography.
Gileadi, O; Burgess-Brown, NA; Colebrook, SM; Berridge, G; Savitsky, P; Smee, CE; Loppnau, P; Johansson, C; Salah, E; Pantic, NH;
Methods in Molecular Biology. 2008 426:221-246. doi: 10.1007/978-1-60327-058-8_14
PMID: 18542867

2007

The scientific impact of the Structural Genomics Consortium: a protein family and ligand-centered approach to medically-relevant human proteins.
Gileadi, O; Knapp, S; Lee, WH; Marsden, BD; Müller, S; Niesen, FH; Kavanagh, KL; Ball, LJ; von Delft, F; Doyle, DA; Oppermann, UC; Sundström, M;
Journal of Structural and Functional Genomics. 2007 8:107-119. doi: 10.1007/s10969-007-9027-2
PMID: 17932789

Crystal structures of histone demethylase JMJD2A reveal basis for substrate specificity.
Ng, SS; Kavanagh, KL; McDonough, MA; Butler, D; Pilka, ES; Lienard, BM; Bray, JE; Savitsky, P; Gileadi, O; von Delft, F; Rose, NR; Offer, J; Scheinost, JC; Borowski, T; Sundstrom, M; Schofield, CJ; Oppermann, U;
Nature. 2007 448:87-91. doi: 10.1038/nature05971
PMID: 17589501

Reversible sequestration of active site cysteines in a 2Fe-2S-bridged dimer provides a mechanism for glutaredoxin 2 regulation in human mitochondria.
Johansson, C; Kavanagh, KL; Gileadi, O; Oppermann, U;
Journal of Biological Chemistry. 2007 282:3077-3082. doi: 10.1074/jbc.M608179200
PMID: 17121859

2006

Direct observation of RuvAB-catalyzed branch migration of single Holliday junctions (vol 101, pg 11605, 2004)
Amit, R; Gileadi, O; Stavans, J;
Proceedings of the National Academy of Sciences of USA. 2006 103:12654-12654. doi:
PMID:

2005

The structural genomics consortium: High-output structure determination
Gileadi, O; Colebrook, S; Burgess, N; Berridge, G; Haroniti, A; Shrestha, B; Smee, C; Sundstrom, M;
The FASEB Journal. 2005 19:A315-A315. doi:
PMID:

2004

Direct observation of RuvAB-catalyzed branch migration of single Holliday junctions.
Amit, R; Gileadi, O; Stavans, J;
Proceedings of the National Academy of Sciences of USA. 2004 101:11605-11610. doi: 10.1073/pnas.0404332101
PMID: 15292509

Electron microscopy of wet tissues: a case study in renal pathology.
Nyska, A; Cummings, CA; Vainshtein, A; Nadler, J; Ezov, N; Grunfeld, Y; Gileadi, O; Behar, V;
Toxicologic Pathology. 2004 32:357-363. doi: 10.1080/01926230490431772
PMID: 15204979

Fat cell precursors from different human fat depots are distinct: Morphological and gene expression profiles
Kirkland, J; Tchkonia, T; Frampton, G; Lenberg, M; Giorgadze, N; Sabban, A; Pirtskhalava, T; Cartwright, M; Gerry, N; Forse, A; Thomou, T; Gileadi, O;
International Journal of Obesity. 2004 28:S99-S99. doi:
PMID:

Scanning electron microscopy of cells and tissues under fully hydrated conditions.
Thiberge, S; Nechushtan, A; Sprinzak, D; Gileadi, O; Behar, V; Zik, O; Chowers, Y; Michaeli, S; Schlessinger, J; Moses, E;
Proceedings of the National Academy of Sciences of USA. 2004 101:3346-3351. doi: 10.1073/pnas.0400088101
PMID: 14988502

A novel method for "Wet" SEM.
Barshack, I; Kopolovic, J; Chowers, Y; Gileadi, O; Vainshtein, A; Zik, O; Behar, V;
Ultrastructural Pathology. 2004 28:29-31. doi:
PMID: 14967596

2003

Squid sperm to clam eggs: imaging wet samples in a scanning electron microscope.
Gileadi, O; Sabban, A;
Biological Bulletin. 2003 205:177-179. doi: 10.2307/1543239
PMID: 14583516

Electron microscopy of wet cells and tissues
Gileadi, O; Behar, V; Ezer, S; Horowitz, M; Nechushtan, A; Sabban, A; Sprinzak, D; Vainshtein, A; Zik, O;
The FASEB Journal. 2003 17:A1080-A1080. doi:
PMID:

Scanning electron microscopy of wet biological specimens
Gileadi, O; Behar, V; Ezer, S; Horowitz, M; Nechushtan, A; Sabban, A; Sprinzak, D; Vainshtein, A; Zik, O;
Microscopy and Microanalysis. 2003 9:486-487. doi:
PMID:

2002

Compaction of single DNA molecules by histone-like binding protein HU.
Schnurr, B; Amit, R; Oppenheim, AB; Gileadi, O; Stavans, J;
Biophysical Journal. 2002 82:51A-51A. doi:
PMID:

2001

Objective-type dark-field illumination for scattering from microbeads.
Braslavsky, I; Amit, R; Jaffar Ali, BM; Gileadi, O; Oppenheim, A; Stavans, J;
Applied Optics. 2001 40:5650-5657. doi:
PMID: 18364854

Compaction of single DNA molecules induced by binding of integration host factor (IHF).
Ali, BM; Amit, R; Braslavsky, I; Oppenheim, AB; Gileadi, O; Stavans, J;
Proceedings of the National Academy of Sciences of USA. 2001 98:10658-10663. doi: 10.1073/pnas.181029198
PMID: 11535804

Involvement of yeast carboxy-terminal domain kinase I (CTDK-I) in transcription elongation in vivo.
Jona, G; Wittschieben, BO; Svejstrup, JQ; Gileadi, O;
Gene. 2001 267:31-36. doi:
PMID: 11311553

Autophosphorylation restrains the apoptotic activity of DRP-1 kinase by controlling dimerization and calmodulin binding.
Shani, G; Henis-Korenblit, S; Jona, G; Gileadi, O; Eisenstein, M; Ziv, T; Admon, A; Kimchi, A;
The EMBO Journal. 2001 20:1099-1113. doi: 10.1093/emboj/20.5.1099
PMID: 11230133

2000

Distinct regions of MAT1 regulate cdk7 kinase and TFIIH transcription activities.
Busso, D; Keriel, A; Sandrock, B; Poterszman, A; Gileadi, O; Egly, JM;
Journal of Biological Chemistry. 2000 275:22815-22823. doi: 10.1074/jbc.M002578200
PMID: 10801852

Glucose starvation induces a drastic reduction in the rates of both transcription and degradation of mRNA in yeast.
Jona, G; Choder, M; Gileadi, O;
Biochimica et Biophysica Acta: international journal of biochemistry and biophysics. 2000 1491:37-48. doi:
PMID: 10760568

Subunit interactions in yeast transcription/repair factor TFIIH. Requirement for Tfb3 subunit in nucleotide excision repair.
Feaver, WJ; Huang, W; Gileadi, O; Myers, L; Gustafsson, CM; Kornberg, RD; Friedberg, EC;
Journal of Biological Chemistry. 2000 275:5941-5946. doi:
PMID: 10681587

Erratum: Cak1 is required for Kin28 phosphorylation and activation in vivo (Molecular and Cellular Biology (1999) 19:12 (6365-6373))
Espinoza, FH; Farrell, A; Nourse, JL; Chamberlin, HM; Gileadi, O; Morgan, DO;
Molecular and Cellular Biology. 2000 20:1898-. doi: 10.1128/MCB.20.5.1898-1898.2000
PMID:

1999

An unusual eukaryotic protein phosphatase required for transcription by RNA polymerase II and CTD dephosphorylation in S. cerevisiae.
Kobor, MS; Archambault, J; Lester, W; Holstege, FC; Gileadi, O; Jansma, DB; Jennings, EG; Kouyoumdjian, F; Davidson, AR; Young, RA; Greenblatt, J;
Molecular Cell. 1999 4:55-62. doi:
PMID: 10445027

1998

Cak1 is required for Kin28 phosphorylation and activation in vivo.
Espinoza, FH; Farrell, A; Nourse, JL; Chamberlin, HM; Gileadi, O; Morgan, DO;
Molecular and Cellular Biology. 1998 18:6365-6373. doi:
PMID: 9774652

Analysis of the Ac promoter: structure and regulation.
Fridlender, M; Sitrit, Y; Shaul, O; Gileadi, O; Levy, AA;
Molecular Genetics and Genomics: an international journal. 1998 258:306-314. doi:
PMID: 9645437

1995

Partial truncation of the yeast RNA polymerase II carboxyl-terminal domain preferentially reduces expression of glycolytic genes.
Meisels, E; Gileadi, O; Corden, JL;
Journal of Biological Chemistry. 1995 270:31255-31261. doi:
PMID: 8537392

1994

Yeast TFIIE. Cloning, expression, and homology to vertebrate proteins.
Feaver, WJ; Henry, NL; Bushnell, DA; Sayre, MH; Brickner, JH; Gileadi, O; Kornberg, RD;
Journal of Biological Chemistry. 1994 269:27549-27553. doi:
PMID: 7961670

1993

YEAST RNA POLYMERASE-II TRANSCRIPTION - STRUCTURE, MECHANISM AND REGULATION
KORNBERG, R; CHASMAN, D; DARST, S; DAVID, P; EDWARDS, A; FEAVER, W; FLANAGAN, P; GILEADI, O; HENRY, N; KELLEHER, R; LI, Y; LORCH, Y; MEREDITH, G; POGLITSCH, C; SAYRE, M; TSCHOCHNER, H;
Journal of Cellular Biochemistry. 1993 :55-55. doi:
PMID:

1992

Identification of human satellite DNA sequences associated with chemically resistant nonhistone polypeptide adducts.
Pfütz, M; Gileadi, O; Werner, D;
Chromosoma: biology of the nucleus. 1992 101:609-617. doi:
PMID: 1424985

Cloning of a subunit of yeast RNA polymerase II transcription factor b and CTD kinase.
Gileadi, O; Feaver, WJ; Kornberg, RD;
Science. 1992 257:1389-1392. doi:
PMID: 1445600

Transcription of the H19 gene in differentiating cytotrophoblasts from human placenta.
Rachmilewitz, J; Gileadi, O; Eldar-Geva, T; Schneider, T; de-Groot, N; Hochberg, A;
Molecular Reproduction and Development. 1992 32:196-202. doi: 10.1002/mrd.1080320303
PMID: 1497869

YEAST RNA POLYMERASE-II TRANSCRIPTION - ENZYMATIC-ACTIVITIES OF GENERAL INITIATION-FACTORS, 3-D STRUCTURES OF INITIATION-FACTORS AND RNA-POLYMERASE, AND MECHANISM OF TRANSCRIPTIONAL ACTIVATION
KORNBERG, R; CHASMAN, D; DARST, S; EDWARDS, A; FEAVER, W; FLANAGAN, P; GILEADI, O; HINDS, D; KELLEHER, R; MEREDITH, G; LI, Y; LORCH, Y; PARK, B; SAYRE, M; TSCHOCHNER, H;
The FASEB Journal. 1992 6:A274-A274. doi:
PMID:

1991

CTD kinase associated with yeast RNA polymerase II initiation factor b.
Feaver, WJ; Gileadi, O; Li, Y; Kornberg, RD;
Cell. 1991 67:1223-1230. doi:
PMID: 1836979

Purification and characterization of yeast RNA polymerase II transcription factor b.
Feaver, WJ; Gileadi, O; Kornberg, RD;
Journal of Biological Chemistry. 1991 266:19000-19005. doi:
PMID: 1918015

1988

THE EXPRESSION OF PROTOONCOGENES AND 2'-5' OLIGOADENYLATE SYNTHETASE IN DIFFERENTIATING HUMAN TROPHOBLASTIC CELLS-INVITRO
GILEADI, O; SCHNEIDER, T; CHEBATH, J; DEGROOT, N; HOCHBERG, A;
. 1988 798:251-260. doi:
PMID:

1984

Location of RNase and RNase inhibitor on free cytoplasmic mRNA-protein particles from human placenta.
Gileadi, O; Lorberboum, H; de Groot, N; Hochberg, AA;
Molecular Biology Reports: an international journal on molecular and cellular biology. 1984 9:241-244. doi:
PMID: 6708950

1982

Phosphorylation of proteins in cultured human placenta
Galski, H; Weinstein, D; Gileadi, O;
Biochemistry International. 1982 5:137-143. doi:
PMID:

1111

High-performance liquid chromatography separation and intact mass analysis of detergent-solubilized integral membrane proteins
Berridge, G; Chalk, R; Burgess-Brown, NA; Carpenter, EP; Gileadi, O;
Analytical Biochemistry. 1111 :-. doi: 10.1016/j.ab.2010.11.008
PMID:

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