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The SGC is providing expression and purification protocols for the following proteins that have been successfully purified in our laboratories. These protocols are in addition to those we regularly publish as part of our data packages for deposited structures (www.thesgc.org/structures). To request a protocol, please send an email to proteinprotocols@thesgc.org stating the protein name/acronym as listed.
Please note: The SGC endeavours to provide accurate materials and methods. However, as lab practices may vary, feel free to contact us if you require additional information or if you encounter any problems reproducing the procedures.
Currently available protein production materials and methods
| Target ID | Target Description | Domain Information |
|---|---|---|
| AACS | Human Acetoacetyl-CoA Synthetase | Transferase domain |
| AKR1D1 | Human Aldo-keto reductase family 1 member D1 | Aldo/keto reductase domain |
| ALDH5A1 | Human Aldehyde dehydrogenase 5 family, member A1 | Aldehyde dehydrogenase domain |
| AMPKA1 | Human kinase AMPKA | Kinase domain |
| ARAP3 | Human ArfGAP with RhoGAP domain, ankyrin repeat and PH domain | PH-like and ArfGap domains |
| ATG5 | Human ATG5L | Full length |
| ATXN1L | Human Ataxin-1-like | aa 461-584 (ataxin domain) |
| BIRC3 | Human Baculoviral IAP repeat-containing protein 3; Inhibitor of apoptosis protein 1 | 1st BIR domain |
| CA5B | Human Carbonic Anhydrase 5B | aa 61-297 |
| CDH1 | Human Fizzy/cell division cycle 20 related 1 | Full length |
| CHD2 | Human Chromodomain-helicase-DNA-binding protein 2 | Tandem Chromo1 and Chromo2 domains |
| CHD3 | Human Chromodomain-helicase-DNA-binding protein 3 | Tandem PhD1, PHD2, Chromo 1 and Chromo2 domains |
| CHD4 | Human Chromodomain-helicase-DNA-binding protein 4 | Tandem PHD2, Chromo1 and Chromo2 domains |
| CHD5 | Human Chromodomain-helicase-DNA-binding protein 5 | Tandem PHD2, Chromo1 and Chromo2 domains |
| CHD6 | Human Chromodomain-helicase-DNA-binding protein 6 | Chromo1 and Chromo2 domains |
| CHD7 | Human Chromodomain-helicase-DNA-binding protein 7 | Chromo1 and Chromo2 domains |
| CHEK2 | Human CHK2 checkpoint homolog (S. pombe) [isoform a] | Kinase domain |
| CHFR isoform 1 | Human checkpoint with forkhead and RING finger domains | RING domain |
| CIDR-1a | Plasmodium falciparum erythrocyte membrane protein 1 | Cysteine-Rich Interdomain Region 1a |
| COPS5 | Human Constitutive photomorphogenic homolog subunit 5 | aa 9 to 309 |
| CRYZL1 | Human crystallin, zeta | Full length |
| CYP4x1 | Human Cytochrome P450 4X1 | Full length protein |
| DPP10 | Human Inactive dipeptidyl peptidase 10 | Almost full length |
| EDD1 | Human Ubiquitin protein ligase E3 component n-recognin 5 | HECT domain |
| EIF4BP1 | Human Eukaryotic translation initiation factor 4E-binding protein 1 | Almost full length |
| ELMO3 | Human engulfment and cell motility 3 | DUF3361 and ELMO_CED12 |
| FGFR4 | Human Fibroblast growth factor receptor 4 isoform 1 precursor | Kinase domain |
| FOXP3 | Human forkhead box P3 | Forkhead domain |
| GMIP | Human GEM interacting protein | BAR domain |
| GRAIL | Gene related to anergy in lymphocytes | RING domain |
| GRM2 | Human Metabotropic glutamate receptor 2 | Extracellular ligand binding domain |
| GRM3 | Human Metabotropic glutamate receptor 3 | Extracellular ligand binding domain |
| GRM8 | Human Metabotropic glutamate receptor 8 | Extracellular ligand binding domain |
| GRN | Human granulin | Full length, missing N-terminal 17 residues |
| GUK1 | Human Guanylate Kkinase 1 | Full length |
| Hace1 | Human HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 | HECT domain |
| HECW1 | Human HECT, C2 and WW domain-containing protein 1 | N-terminus |
| HLTF | Human helicase-like transcription factor | RING domain |
| IL18BP | Human Interleukin-18-binding protein | Full length |
| ISCA1 | Human Iron-sulfur cluster assembly homologue 1 | Almost full length |
| ITSN2 | Human Intersectin-2 | 4th and 5th SH3s+RhoGEF+PH+C2 domains |
| Lamin B2 | Human Lamin B2 | Coiled-coil domain |
| LIG3 | Human DNA Ligase III | Residues 260-834 |
| LIPA | Human Lysosomal acid lipase/cholesteryl ester hydrolase | Full length protein |
| LIPT1 | Human Lipoyltransferase 1, mitochondrial | aa 31-373 |
| LmPK | Leishmania major protein kinase | Full length |
| MAP2K2 | Human mitogen-activated protein kinase kinase 2 | Kinase domain |
| MAPK13 | Human mitogen-activated protein kinase 13 | Kinase domain |
| MAPK3 | Human mitogen-activated protein kinase 3 | Kinase domain |
| MAPK8 | Human mitogen-activated protein kinase 8 [isoform 2] | Kinase domain |
| MAPK9 | Human mitogen-activated protein kinase 9 [isoform 1] | Kinase domain |
| MENIN | Human Multiple endocrine neoplasia | N-terminal 542 aa of isoform 2 |
| MGC90512 | Human alkylation repair homolog 2 | Jmj domain |
| MICAL1 | Human Microtubule associated monoxygenase | C-terminal domain |
| MLL3 | Human Myeloid/lymphoid or mixed-lineage leukemia, translocated to, 3 | Yeats domain |
| MLLT1 | Human Myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila) | Yeats domain |
| MPP1 | Human membrane protein, palmitoylated 1 | Full length |
| NANOS | Human Nanos homologue 2 | Zinc finger domain |
| Nedd4 | Human Neural precursor cell expressed, developmentally down-regulated 4 | HECT domain |
| NME7 | Human non-metastatic cells 7 | Residues 49-373 |
| NPEPPS | Human Aminopeptidase puromycin sensitive | Full length |
| NR2E1 | Human Nuclear receptor subfamily 2 group E member 1 | Nuclear receptor DNA-binding domain |
| NS3HHCV | Human NS3 protease/helicase from hepatitis C virus | Full length processed protein |
| OBP2B | Human Odorant-binding protein IIb | aa 20-166 |
| OLR1 | Human oxidised low density lipoprotein (lectin-like) receptor 1 | Extracellular C-terminal domain |
| OSR1 | Human oxidative-stress responsive 1 | Kinase domain |
| P4HB | Human Proline 4-hydroxylase beta subunit | Full length |
| PAPPA | Human PAPPA Pappalysin-1 | N-terminus including LamG and metalloprotease domains |
| PbCDPK1 | Plasmodium berghei calcium dependent protein kinase 1 | Full length protein |
| PfCDPK1 | Plasmodium falciparum calcium dependent protein kinase 1 | Full length protein |
| PfCDPK2 | Plasmodium falciparum calcium dependent protein kinase 2 | Kinase and CAD domains |
| PfCDPK4 | Plasmodium falciparum calcium dependent protein kinase 4 | Full length |
| PfEK | Plasmodium falciparum ethanolamine kinase | Full length |
| PfGMPS | Plasmodium falciparum guanosine monophosphate synthase | Full length |
| PfHsp83 | Plasmodium falciparum heat shock protein 83 | N-terminal domain |
| PFKB | Human Phosphofructokinase | N-terminal domain |
| PFKP | Human Phosphofructokinase, platelet | Almost full length (aa 25- 762) |
| PfPK7 | Plasmodium falciparum protein kinase K | Full length |
| PfPK9 | Plasmodium falciparum protein kinase 9 kinase domain | Kinase domain |
| PfTRAP1 | Plasmodium falciparum Hsp90-like chaperone TRAP1 | N-terminal domain |
| PfUMPCHMP | Plasmodium falciparum UMP-CMP kinase | C-terminus |
| PHF14 | Human PHD finger protein 14 | PHD finger domain |
| PLD3 | Human Phospholipase D family, member 3 | Extracellular domain |
| PLK4 | Human polo-like kinase 4 (Drosophila) | Kinase domain |
| Plus-3RTF1 | Human RNA polymerase-associated protein RTF1 homolog | Plus-3 |
| PRDX3 | Human Peroxiredoxin 3 | Residues 69-256 |
| PRKCH | Human kinase PKC eta | Kinase domain |
| PSAPL1 | Human Prosaposin-Like 1 | Saposin A-like domain |
| PvCDPK1 | Plasmodium vivax calcium-dependent protein kinase 1 | Full length protein |
| RNF8 | Human ring finger protein (C3HC4 type) 8 | RING domain |
| ROR2 | Human Receptor tyrosine kinase-like orphan receptor 2 | Kinase domain |
| SEMA3A | Human Semaphorin 3A | SEMA domain |
| SGEF | Human Src homology 3 domain-containing guanine nucleotide exchange factor | RhoGEF/DH domain |
| SIRT1 | Human NAD-dependent deacetylase sirtuin-1 | Full length |
| SMN1 | Human Survival of motor neuron 1 | Tudor domain |
| SMNDC1 | Human Survival of motor neuron-related-splicing factor 30 | Tudor domain |
| SMURF1 | Human Smad ubiquitination regulatory factor 1 | HECT domain |
| SOCS3/ElonginC/ElonginB Complex | Human CIS3 (SOCS3),TCEB1 (ElonginC),TCEB2 (ElonginB) | Complex |
| SOCS6/ElonginC/ElonginB Complex | Human Suppressor of cytokine signaling 6 in complex with Elongin B/C | Complex |
| STAT6 | Human signal transducer and activator of transcription 6 | STAT interaction domain, all-alpha domain, DNA binding domain and SH2 domain |
| STK24 | Human serine/threonine kinase 24 (STE20 homolog, yeast) | Kinase domain |
| TARDBP | Human TAR DNA binding protein | Double RRM domain |
| TgMAPK3 | Toxoplasma gondii mitogen-activated protein kinase | Kinase domain |
| TP73L | Human Tumor protein p73-like | DNA binding domain |
| TRB1 | Human Tribbles homolog 1 | Kinase domain |
| TRB2 | Human kinase TRB2 | Kinase domain |
| TRIM16 | Human Tripartite motif-containing protein 16 | PRY domain and SPRY domain |
| TRIM21(RO52) | Tripartite motif-containing 21 | RING domain |
| TSC1 | Human tuberous sclerosis 1 | N-terminal domain |
| TSC2 | Human GAP domain | tuberin |
| UGCGA | Human UDP-glucose ceramide glucosyltransferase | Transferase domain |
| UL44 | Human herpesvirus 2 envelope glycoprotein C | Ig + Marek_A domains |
| USP10 | Human Ubiquitin specific peptidase 10 | Catalytic domain |
| USP11 | Human ubiquitin specific peptidase 11 | DUSP domain |
| VPS39 | Human Vam6/Vps39-like protein | Clathrin homology containing domain |
| WT1 | Human Wilms tumor 1 isoform A | Zinc finger domain |