SGC Vectors

This table shows data for SGC vectors - click the Clone Vector ID for a PDF information sheet for the vector. Links to AddGene to purchase vectors are in the Description column.

Links to AddGene and Source BioScience to purchase vectors are in the Description column

Link to SGC Oxford Lab Plasmids
Clone Vector ID GenBank Deposition ID SGC Site  Description
p11   Oxford/Toronto E. coli expression vector with N-terminal Hexa-HIS and TEV cleavage
p15   Oxford E. coli expression vector with C-terminal His6 tag and TEV cleavage
p15TV-L EF456736 Toronto E. coli expression vector with N-terminal Hexa-HIS and TEV cleavage available from AddGene
p21a-LIC EF456737 Toronto E. coli expression vector with N-terminal Hexa-HIS and TEV cleavage
p28BIOH-LIC KC164371 Toronto E. coli expression vector with N-terminal AviTag and a C-terminal Hexa-HIS tag
p28BIOHTEV-LIC   Toronto E. coli expression vector with N-terminal TEV cleavable AviTag and a C-terminal Hexa-HIS tag
pAB-LIC   Toronto Baculovirus expression vector for secretion with N-terminal Honeybee melittin signal and C-terminal 8xHis tag
pACYC-GST   Toronto E. coli pACYC based expression vector with N-terminal TEV cleavable GST-tag
pACYC-LIC+   Oxford/Toronto E. coli pACYC based expression vector with N-terminal Hexa-HIS and a thrombin protease cleavage site
pBEN1-SGC GU725055 Oxford E. coli expression vector with N-terminal Strep tag and SET1 (solubility enhancement tag) available from Source BioScience Life Sciences
pCD33-CH   Toronto Mammalian expression vector with N-terminal CD33 signal peptide and C-terminal Hexa-HIS
pCDF-BirA JF914075 Oxford E. coli co-expression vector containing the biotin-protein ligase gene
pCDF-Duet-1-LIC1   Toronto E. coli co-expression vector derived from pCDF-Duet-1with N-terminal His tag followed by TEV cleavage site preceded by 1st promoter. This vector is compatible with pET and pACYC vectors
pCDF-LIC   Oxford E. coli co-expression vector with N-terminal His6 tag and TEV cleavage, compatible with pET and pACYC vectors
pcDNA3-C-Flag-LIC   Oxford Mammalian expression vector with C-terminal Flag tag
pcDNA3-C-GFP-LIC   Oxford Mammalian expression vector with C-terminal GFP tag
pcDNA3-CT10HF-LIC   Oxford Mammalian expression vector with C-terminal His10-Flag tags and TEV cleavage
pcDNA3-N-Flag-LIC   Oxford Mammalian expression vector with N-terminal Flag tag
pcDNA3-N-GFP-LIC   Oxford Mammalian expression vector with N-terminal GFP tag
pCOEX-1   Oxford E. coli co-expression vector with N-terminal His7 tag and TEV cleavage, pACYC backbone
pCW-LIC EF460848 Toronto E. coli expression vector available from AddGene
pER-NH   Toronto Mammalian based expression vector with N-terminal ER signal peptide and Hexa-HIS
pET15-MHL EF456738 Toronto E. coli expression vector with N-terminal Hexa-HIS and TEV cleavage available from AddGene
pET28a-LIC EF442785 Toronto E. coli expression vector with N-terminal Hexa-HIS and Thrombin cleavage available from AddGene
pET28-CFP-YFP   Toronto E. coli expression vector with N-terminal Hexa-HIS, CFP and TEV cleavage, and C-terminal YFP
pET28-CUb   Toronto E. coli expression vector with N-terminal Hexa-HIS and TEV cleavage and coexpression of ubiquitin at C-terminal.
pET28-FLAG   Toronto E. coli expression vector with N-terminal Hexa-HIS, FLAG tag, and TEV cleavage
pET28GST-LIC EF456739 Toronto E. coli expression vector with N-terminal Hexa-HIS, GSTtag, and Thrombin cleavage available from AddGene
pET28GSTlinker-LIC   Toronto E. coli expression vector with a long linker between N-terminal GST tag and TEV cleavage site. The linker is designed to leave GST-tag free in DNA binding screening experiments
pET28-MHL EF456735 Toronto E. coli expression vector with N-terminal Hexa-HIS and TEV cleavage available from AddGene
pET28-MKH8SUMO   Toronto E. coli expression vector with N-terminal Hexa-HIS, thrombin,SUMO and TEV cleavage
pET28-SBP   Toronto E. coli expression vector with N-terminal Hexa-HIS and SBP tag
pET28-SBP-TEV    Toronto E. coli expression vector with N-terminal Hexa-HIS, SBP tag and TEV cleavage available from AddGene
pET32a-LIC EF442786 Toronto E. coli expression vector with N-terminal Trx-tag, 6X His-tag,  thrombin site, S-tag, and TEV
pETDuet-1-LIC1   Toronto E. coli co-expression vector derived from pET-Duet-1with N-terminal His tag followed by TEV cleavage site preceded by 1st promoter. This vector is compatible with pACYC vector.
pFB-6HZB   Oxford Baculovirus expression vector with N-terminal His6-ZB tags and TEV cleavage
pFB-CT10HF-LIC   Oxford Baculovirus expression vector with C-terminal His10-Flag tags and TEV cleavage available from AddGene and Source BioScience Life Sciences
pFBD-BirA   Toronto Baculovirus expression vector with N-terminal AviTag, C-terminal Hexa-HIS and Coexpression of BirA
pFB-HGT-LIC   Oxford Baculovirus expression vector with N-terminal His6-GST tags and TEV cleavage
pFBIP-C   Toronto Baculovirus expression vector with N-terminal BiP signal peptide, Hexa-His, and TEV cleavage site
pFBIP-N   Toronto Baculovirus expression vector with N-terminal BiP signal peptide and C-terminal Hexa-His
pFB-LIC-Bse EF199842 Oxford Baculovirus expression vector with N-terminal His6 tag and TEV cleavage available from AddGene and Source BioScience Life Sciences
pFB-Mam-CT10HF-LIC   Oxford BacMam expression vector C-terminal His10-Flag tags and TEV cleavage
pFB-NHT-CF-Bse   Oxford Baculovirus expression vector with N-terminal His6, TEV cleavage and C-terminal Flag tag
pFBOH-avi-TEV-LIC   Toronto Baculovirus expression vector with N-terminal Hexa-His, AviTag, and TEV cleavage
pFBOH-GST   Toronto Baculovirus expression vector with N-terminal GST tag, Hexa-HIS and TEV cleavage
pFBOH-LIC EF456740 Toronto Baculovirus expression vector with N-terminal Hexa-HIS and Thrombin cleavage available from AddGene
pFBOH-MHL   Toronto Baculovirus expression vector with N-terminal Hexa-HIS and TEV cleavage
pFBOH-SBP-TEV-LIC   Toronto Baculovirus expression vector with N-terminal Hexa-HIS,  SBP-tag, and TEV cleavage
pFBOH-SUMOstar   Toronto Baculovirus expression vector with N-terminal Hexa-HIS and SUMOstar fusion protein
pFBOH-SUMOstar-TEV   Toronto Baculovirus expression vector with N-terminal Hexa-HIS, SUMOstar fusion protein, and TEV cleavage
pFB-Sec-NH   Oxford Baculovirus secretion vector with gp64 signal peptide, N-terminal His6 tag and TEV cleavage available from AddGene and Source BioScience Life Sciences
pFB-ZB JF682518 Oxford Baculovirus expression vector with N-terminal His10-ZB tags and TEV cleavage
pFGP64-C   Toronto Baculovirus expression vector for secretion with N-terminal Autographa californica nuclear polyhedrosis virus gp64 signal peptide and C-terminal Hexa-His.
pFGP64-N   Toronto Baculovirus expression vector for secretionwith N-terminal Autographa californica nuclear polyhedrosis virus gp64 signal peptide,  Hexa-His, and TEV cleavage
pFHMSP-LIC-C   Toronto Baculovirus expression vector for secretion with C-terminal Hexa-HIS available from AddGene
pFHMSP-LIC-N   Toronto Baculovirus expression vector for secretion with N-terminal Hexa-HIS and TEV cleavage available from AddGene
pFHMSPN-avi-TEV-LIC   Toronto Baculovirus expression vector for secretion with N-terminal Hexa-HIS, Avi-tag and TEV cleavage
pFHMSPN-SBP-TEV-LIC   Toronto Baculovirus expression vector for secretion with N-terminal Hexa-HIS, SBP-tag and TEV cleavage
pFSTREP-C   Toronto Baculovirus expression vector  for secretion with N-terminal Apis mellifera melittin signal peptide and C-terminal Strep-tag
pFSTREP-N   Toronto Baculovirus expression vector  for secretion with N-terminal Apis mellifera melittin signal peptide, Strep-tag, and TEV cleavage
pFTRYP-C   Toronto Baculovirus expression vector  for secretion with N-terminal Homo sapiens trypsin 2 signal peptide and a C-terminal Hexa-His
pFTRYP-EK-C   Toronto Baculovirus expression vector  for secretion with N-terminal Homo sapiens trypsin 2 signal peptide, enterokinase cleavage site and C-terminal Hepta-His
pFTRYP-EK-N   Toronto Baculovirus expression vector  for secretion with N-terminal Homo sapiens trypsin 2 signal peptide, enterokinase cleavage site , Hexa-His, and TEV cleavage
pFTRYP-N   Toronto Baculovirus expression vector  for secretion with N-terminal Homo sapiens trypsin 2 signal peptide, Hexa-His, and TEV cleavage
pFUSE-HEAVY KC176268 Toronto Mammalian expression vector for IgG HC production
pFUSE-LIGHT KC176267 Toronto Mammalian expression vector for IgG LC production
pGTvL1-SGC   Oxford E. coli expression vector with GST and TEV cleavage available from AddGene and Source BioScience Life Sciences
pGTVL2 JF522100 Oxford E. coli expression vector with His6-GST and TEV cleavage 
pLIC-SGC1   Oxford E. coli expression vector with His6 and TEV cleavage available from AddGene and Source BioScience Life Sciences
pNH-TrxT GU269914 Oxford E. coli expression vector with N-terminal His6-Thioredoxin and TEV cleavage available from AddGene and Source BioScience Life Sciences
pNIC28-Bsa4 EF198106 Oxford E. coli expression vector with N-terminal His6 tag and TEV cleavage available from AddGene and Source BioScience Life Sciences
pNIC-Bio1   Oxford E. coli expression vector with N-terminal His10, TEV cleavage and C-terminal Avi tag
pNIC-Bio2 JF912191 Oxford E. coli expression vector with N-terminal His10, TEV cleavage and C-terminal Avi tag
pNIC-Bio3 JN792439 Oxford E. coli expression vector with N-terminal His6, TEV cleavage and C-terminal Avi tag
pNIC-CH EF199843 Oxford E. coli expression vector with C-terminal His6 tag available from AddGene and Source BioScience Life Sciences
pNIC-CTHO   Oxford E. coli expression vector with C-terminal His6 tag and TEV cleavage
pNIC-CTHF EF199844 Oxford E. coli expression vector with C-terminal His6-Flag tags and TEV cleavage available from AddGene and Source BioScience Life Sciences
pNIC-H102 JF912192 Oxford E. coli expression vector with N-terminal His10 tag and TEV cleavage
pNIC-NHT-CF EF199845 Oxford E. coli expression vector with N-terminal His6, TEV cleavage and C-terminal Flag tag
pNIC-ZB GU452710 Oxford E. coli expression vector with N-terminal His6-ZB tags and TEV cleavage available from AddGene and Source BioScience Life Sciences
pSFV4 KC164372 Toronto E. coli expression vector for FABs with AviTag
pTRC-LIC EF460847 Toronto E. coli expression vector
pTvHR21-SGC   Oxford E. coli expression vector with C-terminal His6 tag (for recombinase cloning) available from AddGene and Source BioScience Life Sciences
pFB-Nflag-LIC   Toronto Baculovirus expression vector with N-terminal Flag tag followed by TEV cleavage site
pFBD-LIC1   Toronto Baculovirus dual expression vector with N-terminal His tag followed by a thrombin cleavage site preceded by Polyhedrin promoter. The P10 promoter is untagged, but the Nco I site under P10 is destroyed which gives more flexibility of removing or replacing His-tag by Nco I and BseR I cut.
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