The name stands for Amplified Luminescent Proximity Homogeneous Assay. Binding of molecules captured on the beads leads to an energy transfer from one bead to the other, ultimately producing a luminescent/fluorescent signal. The assay can be used for studying the protein-protein interactions (PPI) as shown below. The PPI inhibitor will conceivably disrupt complex formation in concentration dependent manner resulting in the disappearance of the signal. Alternatively, assay can rely on the detection of the enzymatic reaction product. Addition of enzyme inhibitor will decrease the product production and again, the Alpha screen signal.
Also known as Thermal Shift Assay. The assay principle is based on the stabilization of the native protein structure upon the ligand binding. The rank order of ligand binding and the estimate of the binding energy can be obtained by comparing the Tm values of the apo-protein and protein-ligand complexes.
This is a label-free direct detection method for study protein-protein and protein-ligand interaction. The applicability and information output is similar to surface plasmon resonance method. OctetRed384 is 16-channel medium to high throughput machine operating with 384-well sample plates.