Protein Production Materials and Methods

The SGC is providing expression and purification protocols for the following proteins that have been successfully purified in our laboratories. These protocols are in addition to those we regularly publish as part of our data packages for deposited structures ( To request a protocol, please send an email to stating the protein name/acronym as listed.

Please note: The SGC endeavours to provide accurate materials and methods. However, as lab practices may vary, feel free to contact us if you require additional information or if you encounter any problems reproducing the procedures.

Currently available protein production materials and methods

Target IDTarget DescriptionDomain Information
AACSHuman Acetoacetyl-CoA SynthetaseTransferase domain
AKR1D1Human Aldo-keto reductase family 1 member D1Aldo/keto reductase domain
ALDH5A1Human Aldehyde dehydrogenase 5 family, member A1Aldehyde dehydrogenase domain
AMPKA1Human kinase AMPKAKinase domain
ARAP3Human ArfGAP with RhoGAP domain, ankyrin repeat and PH domainPH-like and ArfGap domains
ATG5Human ATG5LFull length
ATXN1LHuman Ataxin-1-likeaa 461-584 (ataxin domain)
BIRC3Human Baculoviral IAP repeat-containing protein 3; Inhibitor of apoptosis protein 11st BIR domain
CA5BHuman Carbonic Anhydrase 5Baa 61-297
CDH1Human Fizzy/cell division cycle 20 related 1Full length
CHD2Human Chromodomain-helicase-DNA-binding protein 2Tandem Chromo1 and Chromo2 domains
CHD3Human Chromodomain-helicase-DNA-binding protein 3Tandem PhD1, PHD2, Chromo 1 and Chromo2 domains
CHD4Human Chromodomain-helicase-DNA-binding protein 4Tandem PHD2, Chromo1 and Chromo2 domains
CHD5Human Chromodomain-helicase-DNA-binding protein 5Tandem PHD2, Chromo1 and Chromo2 domains
CHD6Human Chromodomain-helicase-DNA-binding protein 6Chromo1 and Chromo2 domains
CHD7Human Chromodomain-helicase-DNA-binding protein 7Chromo1 and Chromo2 domains
CHEK2Human CHK2 checkpoint homolog (S. pombe) [isoform a]Kinase domain
CHFR isoform 1Human checkpoint with forkhead and RING finger domainsRING domain
CIDR-1aPlasmodium falciparum erythrocyte membrane protein 1Cysteine-Rich Interdomain Region 1a
COPS5Human Constitutive photomorphogenic homolog subunit 5aa 9 to 309
CRYZL1Human crystallin, zetaFull length
CYP4x1Human Cytochrome P450 4X1Full length protein
DPP10Human Inactive dipeptidyl peptidase 10Almost full length
EDD1Human Ubiquitin protein ligase E3 component n-recognin 5HECT domain
EIF4BP1Human Eukaryotic translation initiation factor 4E-binding protein 1Almost full length
ELMO3Human engulfment and cell motility 3DUF3361 and ELMO_CED12
FGFR4Human Fibroblast growth factor receptor 4 isoform 1 precursorKinase domain
FOXP3Human forkhead box P3Forkhead domain
GMIPHuman GEM interacting proteinBAR domain
GRAILGene related to anergy in lymphocytesRING domain
GRM2Human Metabotropic glutamate receptor 2Extracellular ligand binding domain
GRM3Human Metabotropic glutamate receptor 3Extracellular ligand binding domain
GRM8Human Metabotropic glutamate receptor 8Extracellular ligand binding domain
GRNHuman granulinFull length, missing N-terminal 17 residues
GUK1Human Guanylate Kkinase 1Full length
Hace1Human HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1HECT domain
HECW1Human HECT, C2 and WW domain-containing protein 1N-terminus
HLTFHuman helicase-like transcription factorRING domain
IL18BPHuman Interleukin-18-binding proteinFull length
ISCA1Human Iron-sulfur cluster assembly homologue 1Almost full length
ITSN2Human Intersectin-24th and 5th SH3s+RhoGEF+PH+C2 domains
Lamin B2Human Lamin B2Coiled-coil domain
LIG3Human DNA Ligase IIIResidues 260-834
LIPAHuman Lysosomal acid lipase/cholesteryl ester hydrolaseFull length protein
LIPT1Human Lipoyltransferase 1, mitochondrialaa 31-373
LmPKLeishmania major protein kinaseFull length
MAP2K2Human mitogen-activated protein kinase kinase 2Kinase domain
MAPK13Human mitogen-activated protein kinase 13Kinase domain
MAPK3Human mitogen-activated protein kinase 3Kinase domain
MAPK8Human mitogen-activated protein kinase 8 [isoform 2]Kinase domain
MAPK9Human mitogen-activated protein kinase 9 [isoform 1]Kinase domain
MENINHuman Multiple endocrine neoplasiaN-terminal 542 aa of isoform 2
MGC90512Human alkylation repair homolog 2Jmj domain
MICAL1Human Microtubule associated monoxygenaseC-terminal domain
MLL3Human Myeloid/lymphoid or mixed-lineage leukemia, translocated to, 3Yeats domain
MLLT1Human Myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila)Yeats domain
MPP1Human membrane protein, palmitoylated 1Full length
NANOSHuman Nanos homologue 2Zinc finger domain
Nedd4 Human Neural precursor cell expressed, developmentally down-regulated 4HECT domain
NME7Human non-metastatic cells 7Residues 49-373
NPEPPSHuman Aminopeptidase puromycin sensitiveFull length
NR2E1Human Nuclear receptor subfamily 2 group E member 1Nuclear receptor DNA-binding domain
NS3HHCVHuman NS3 protease/helicase from hepatitis C virusFull length processed protein
OBP2BHuman Odorant-binding protein IIbaa 20-166
OLR1Human oxidised low density lipoprotein (lectin-like) receptor 1Extracellular C-terminal domain
OSR1Human oxidative-stress responsive 1Kinase domain
P4HBHuman Proline 4-hydroxylase beta subunitFull length
PAPPAHuman PAPPA Pappalysin-1N-terminus including LamG and metalloprotease domains
PbCDPK1Plasmodium berghei calcium dependent protein kinase 1Full length protein
PfCDPK1Plasmodium falciparum calcium dependent protein kinase 1Full length protein
PfCDPK2Plasmodium falciparum calcium dependent protein kinase 2Kinase and CAD domains
PfCDPK4Plasmodium falciparum calcium dependent protein kinase 4Full length
PfEKPlasmodium falciparum ethanolamine kinaseFull length
PfGMPSPlasmodium falciparum guanosine monophosphate synthaseFull length
PfHsp83Plasmodium falciparum heat shock protein 83N-terminal domain
PFKBHuman PhosphofructokinaseN-terminal domain
PFKPHuman Phosphofructokinase, plateletAlmost full length (aa 25- 762)
PfPK7Plasmodium falciparum protein kinase KFull length
PfPK9Plasmodium falciparum protein kinase 9 kinase domainKinase domain
PfTRAP1Plasmodium falciparum Hsp90-like chaperone TRAP1N-terminal domain
PfUMPCHMPPlasmodium falciparum UMP-CMP kinaseC-terminus
PHF14Human PHD finger protein 14PHD finger domain
PLD3Human Phospholipase D family, member 3Extracellular domain
PLK4Human polo-like kinase 4 (Drosophila)Kinase domain
Plus-3RTF1Human RNA polymerase-associated protein RTF1 homologPlus-3
PRDX3Human Peroxiredoxin 3Residues 69-256
PRKCHHuman kinase PKC etaKinase domain
PSAPL1Human Prosaposin-Like 1Saposin A-like domain
PvCDPK1Plasmodium vivax calcium-dependent protein kinase 1Full length protein
RNF8Human ring finger protein (C3HC4 type) 8RING domain
ROR2Human Receptor tyrosine kinase-like orphan receptor 2Kinase domain
SEMA3AHuman Semaphorin 3ASEMA domain
SGEFHuman Src homology 3 domain-containing guanine nucleotide exchange factorRhoGEF/DH domain
SIRT1Human NAD-dependent deacetylase sirtuin-1Full length
SMN1Human Survival of motor neuron 1Tudor domain
SMNDC1Human Survival of motor neuron-related-splicing factor 30Tudor domain
SMURF1Human Smad ubiquitination regulatory factor 1HECT domain
SOCS3/ElonginC/ElonginB ComplexHuman CIS3 (SOCS3),TCEB1 (ElonginC),TCEB2 (ElonginB)Complex
SOCS6/ElonginC/ElonginB ComplexHuman Suppressor of cytokine signaling 6 in complex with Elongin B/CComplex
STAT6Human signal transducer and activator of transcription 6STAT interaction domain, all-alpha domain, DNA binding domain and SH2 domain
STK24Human serine/threonine kinase 24 (STE20 homolog, yeast)Kinase domain
TARDBPHuman TAR DNA binding proteinDouble RRM domain
TgMAPK3Toxoplasma gondii mitogen-activated protein kinaseKinase domain
TP73LHuman Tumor protein p73-likeDNA binding domain
TRB1Human Tribbles homolog 1Kinase domain
TRB2Human kinase TRB2Kinase domain
TRIM16Human Tripartite motif-containing protein 16PRY domain and SPRY domain
TRIM21(RO52)Tripartite motif-containing 21RING domain
TSC1Human tuberous sclerosis 1N-terminal domain
TSC2Human GAP domaintuberin
UGCGAHuman UDP-glucose ceramide glucosyltransferaseTransferase domain
UL44Human herpesvirus 2 envelope glycoprotein CIg + Marek_A domains
USP10Human Ubiquitin specific peptidase 10Catalytic domain
USP11Human ubiquitin specific peptidase 11DUSP domain
VPS39Human Vam6/Vps39-like proteinClathrin homology containing domain
WT1Human Wilms tumor 1 isoform AZinc finger domain
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