Opher Gileadi

Opher Gileadi

SGC Karolinska

Gileadi

Opher Gileadi

Affiliations

Biography

Opher Gileadi obtained his PhD in Biochemistry at the Hebrew University and was then a postdoctoral fellow at Stanford University Medical School. Subsequently he led a research group at the Weizmann Institute of Science. From 2004-2021 he was both at SGC University of Oxford, first as head of the Biotechnology group, then as Principal Investigator of the Genome Integrity & Repair Group as well as Head of the SGC Kinase Chemical Biology Center at the State University of Campinas (UNICAMP). In 2021 Opher took at position as Executive Director, Protein Science and Structure at Exscientia, but re-join the SGC at Karolinska in 2023.

Opher leads the Protein Science team at SGC Karolinska, focussing on developing antibodies and target-enabling packages (TEPs) for under-investigated proteins of humans and pathogens.

2011

High-performance liquid chromatography separation and intact mass analysis of detergent-solubilized integral membrane proteins.

Berridge G, Chalk R, D'Avanzo N, Dong L, Doyle D, Kim JI, Xia X, Burgess-Brown N, Deriso A, Carpenter EP, Gileadi O

Anal. Biochem.. 2011-3-15 . 410(2):272-80 .doi: 10.1016/j.ab.2010.11.008

PMID: 21093405

2010

Structures of the human GTPase MMAA and vitamin B12-dependent methylmalonyl-CoA mutase and insight into their complex formation.

Froese DS, Kochan G, Muniz JR, Wu X, Gileadi C, Ugochukwu E, Krysztofinska E, Gravel RA, Oppermann U, Yue WW

J. Biol. Chem.. 2010-12-3 . 285(49):38204-13 .doi: 10.1074/jbc.M110.177717

PMID: 20876572

A prominent β-hairpin structure in the winged-helix domain of RECQ1 is required for DNA unwinding and oligomer formation.

Lucic B, Zhang Y, King O, Mendoza-Maldonado R, Berti M, Niesen FH, Burgess-Brown NA, Pike AC, Cooper CD, Gileadi O, Vindigni A

Nucleic Acids Res.. 2010-11-8 . 39(5):1703-17 .doi: 10.1093/nar/gkq1031

PMID: 21059676

Isolated short CTG/CAG DNA slip-outs are repaired efficiently by hMutSbeta, but clustered slip-outs are poorly repaired.

Panigrahi GB, Slean MM, Simard JP, Gileadi O, Pearson CE

Proc. Natl. Acad. Sci. U.S.A.. 2010-7-13 . 107(28):12593-8 .doi: 10.1073/pnas.0909087107

PMID: 20571119

High-throughput production of human proteins for crystallization: the SGC experience.

Savitsky P, Bray J, Cooper CD, Marsden BD, Mahajan P, Burgess-Brown NA, Gileadi O

J. Struct. Biol.. 2010-6-10 . 172(1):3-13 .doi: 10.1016/j.jsb.2010.06.008

PMID: 20541610

Probing the structural basis of RecQ helicase function.

Vindigni A, Marino F, Gileadi O

Biophys. Chem.. 2010-3-20 . 149(3):67-77 .doi: 10.1016/j.bpc.2010.03.012

PMID: 20392558

2009

Structure of the human RECQ1 helicase reveals a putative strand-separation pin.

Pike AC, Shrestha B, Popuri V, Burgess-Brown N, Muzzolini L, Costantini S, Vindigni A, Gileadi O

Proc. Natl. Acad. Sci. U.S.A.. 2009-1-27 . 106(4):1039-44 .doi: 10.1073/pnas.0806908106

PMID: 19151156

2008

High throughput production of recombinant human proteins for crystallography.

Gileadi O, Burgess-Brown NA, Colebrook SM, Berridge G, Savitsky P, Smee CE, Loppnau P, Johansson C, Salah E, Pantic NH

Methods Mol. Biol.. 2008-6-11 . 426:221-46 .doi: 10.1007/978-1-60327-058-8_14

PMID: 18542867

Protein production and purification.

Gräslund S, Nordlund P, Weigelt J, Hallberg BM, Bray J, Gileadi O, Knapp S, Oppermann U, Arrowsmith C, Hui R, Ming J, dhe-Paganon S, Park HW, Savchenko A, Yee A, Edwards A, Vincentelli R, Cambillau C, Kim R, Kim SH, Rao Z, Shi Y, Terwilliger TC, Kim CY, Hung LW, Waldo GS, Peleg Y, Albeck S, Unger T, Dym O, Prilusky J, Sussman JL, Stevens RC, Lesley SA, Wilson IA, Joachimiak A, Collart F, Dementieva I, Donnelly MI, Eschenfeldt WH, Kim Y, Stols L, Wu R, Zhou M, Burley SK, Emtage JS, Sauder JM, Thompson D, Bain K, Luz J, Gheyi T, Zhang F, Atwell S, Almo SC, Bonanno JB, Fiser A, Swaminathan S, Studier FW, Chance MR, Sali A, Acton TB, Xiao R, Zhao L, Ma LC, Hunt JF, Tong L, Cunningham K, Inouye M, Anderson S, Janjua H, Shastry R, Ho CK, Wang D, Wang H, Jiang M, Montelione GT, Stuart DI, Owens RJ, Daenke S, Schütz A, Heinemann U, Yokoyama S, Büssow K, Gunsalus KC

Nat. Methods. 2008-2-1 . 5(2):135-46 .doi: 10.1038/nmeth.f.202

PMID: 18235434