Nicola Burgess-Brown

Principal Investigator

University of Oxford

Nicola Burgess-Brown obtained a First Class degree in Applied Biochemical Sciences from the University of Ulster in 1997 and spent the following year working as a molecular biologist for SmithKline Beecham. She received her Ph.D. in Molecular Microbiology “Quorum Sensing and Regulation of Virulence Gene Expression in Porphyromonas gingivalis” at the University of Nottingham in 2001. Nicola returned to industry to work on high-throughput cloning and validation of therapeutic cancer antigens for Oxford Glycosciences and subsequently Celltech R&D Ltd. Since joining the SGC in 2004, Nicola has been responsible for optimising the high-throughput screening processes from cloning to expression and purification of human proteins for structural and functional studies. In June 2009, Nicola developed a similar pipeline for production of human integral membrane proteins (IMPs) using the baculovirus/ insect cell expression system. Since June 2011, Nicola has been the Principal Investigator of Biotechnology, responsible for all biotech research (working with soluble proteins, membrane proteins and epigenetics targets) for the Oxford site.

Research Areas

The Biotechnology group is primarily responsible for generating the pipeline of clones targeted by the SGC in Oxford and determining using high-throughput (HTP) screening methods which proteins are expressed in a soluble and stable form suitable for structural and functional studies. We have developed and optimised protocols for high-throughput cloning, test expression/purification and mass spectrometry analyses, large scale expression and protein production and validation. We screen a wide range of proteins (intracellular, extracellular and integral membrane proteins) using E. coli, baculovirus/insect cell and mammalian (BacMam) expression systems.
The platform established by the Biotechnology team, has enabled the site at Oxford to generate more than 550 novel human protein structures and 5 integral membrane protein structures. The group collaborates and interacts closely with the other SGC teams, to develop methods for increasing protein expression, parallel processing and driving output. In addition to enhancing protein expression levels, we also aim to reduce our cost per structure by seeking alternative cheaper reagents, consumables and increasing throughput further by miniaturising processes and increasing automation where possible. Discovering ways to successfully produce the most challenging human proteins would open many doors to our long-term goals as an organisation of understanding how proteins function independently, in complexes and also how they interact with drug molecules.

Publications

Tryptophan-Mediated Interactions between Tristetraprolin and the CNOT9 Subunit Are Required for CCR4-NOT Deadenylase Complex Recruitment.
Bulbrook, D; Brazier, H; Mahajan, P; Kliszczak, M; Fedorov, O; Marchese, FP; Aubareda, A; Chalk, R; Picaud, S; Strain-Damerell, C; Filippakopoulos, P; Gileadi, O; Clark, AR; Yue, WW; Burgess-Brown, NA; Dean, JLE;
Journal of Molecular Biology. 2018 430:722-736. doi: 10.1016/j.jmb.2017.12.018
PMID: 29291391

Structures of DPAGT1 Explain Glycosylation Disease Mechanisms and Advance TB Antibiotic Design.
Dong YY, Wang H, Pike ACW, Cochrane SA, Hamedzadeh S, Wyszyński FJ, Bushell SR, Royer SF, Widdick DA, Sajid A, Boshoff HI, Park Y, Lucas R, Liu WM, Lee SS, Machida T, Minall L, Mehmood S, Belaya K, Liu WW, Chu A, Shrestha L, Mukhopadhyay SMM, Strain-Damerell C, Chalk R, Burgess-Brown NA, Bibb MJ, Barry Iii CE, Robinson CV, Beeson D, Davis BG, Carpenter EP
Cell. 2018 175(4):1045-1058.e16. doi: 10.1016/j.cell.2018.10.037
PMID: 30388443

Structure of the polycystic kidney disease TRP channel Polycystin-2 (PC2).
Grieben M, Pike AC, Shintre CA, Venturi E, El-Ajouz S, Tessitore A, Shrestha L, Mukhopadhyay S, Mahajan P, Chalk R, Burgess-Brown NA, Sitsapesan R, Huiskonen JT, Carpenter EP
Nat. Struct. Mol. Biol.. 2016 . doi: 10.1038/nsmb.3343
PMID: 27991905

K2P channel gating mechanisms revealed by structures of TREK-2 and a complex with Prozac.
Dong YY, Pike AC, Mackenzie A, McClenaghan C, Aryal P, Dong L, Quigley A, Grieben M, Goubin S, Mukhopadhyay S, Ruda GF, Clausen MV, Cao L, Brennan PE, Burgess-Brown NA, Sansom MS, Tucker SJ, Carpenter EP
Science. 2015 347(6227):1256-1259. doi: 10.1126/science.1261512
PMID: 25766236

Medium-throughput production of recombinant human proteins: ligation-independent cloning.
Strain-Damerell C, Mahajan P, Gileadi O, Burgess-Brown NA
Methods Mol. Biol.. 2013 1091:55-72. doi: 10.1007/978-1-62703-691-7_4
PMID: 24203324

Medium-throughput production of recombinant human proteins: protein production in insect cells.
Mahajan P, Strain-Damerell C, Gileadi O, Burgess-Brown NA
Methods Mol. Biol.. 2013 1091:95-121. doi: 10.1007/978-1-62703-691-7_6
PMID: 24203326

Plant growth regulator daminozide is a selective inhibitor of human KDM2/7 histone demethylases.
Rose NR, Woon EC, Tumber A, Walport LJ, Chowdhury R, Li XS, King ON, Lejeune C, Ng SS, Krojer T, Chan MC, Rydzik AM, Hopkinson RJ, Che KH, Daniel M, Strain-Damerell C, Gileadi C, Kochan G, Leung IK, Dunford J, Yeoh KK, Ratcliffe PJ, Burgess-Brown N, von Delft F, Muller S, Marsden B, Brennan PE, McDonough MA, Oppermann U, Klose RJ, Schofield CJ, Kawamura A
J. Med. Chem.. 2012 55(14):6639-43. doi: 10.1021/jm300677j
PMID: 22724510

Human SNM1A and XPF-ERCC1 collaborate to initiate DNA interstrand cross-link repair.
Wang AT, Sengerová B, Cattell E, Inagawa T, Hartley JM, Kiakos K, Burgess-Brown NA, Swift LP, Enzlin JH, Schofield CJ, Gileadi O, Hartley JA, McHugh PJ
Genes Dev.. 2011 25(17):1859-70. doi: 10.1101/gad.15699211
PMID: 21896658

High-throughput production of human proteins for crystallization: the SGC experience.
Savitsky P, Bray J, Cooper CD, Marsden BD, Mahajan P, Burgess-Brown NA, Gileadi O
J. Struct. Biol.. 2010 172(1):3-13. doi: 10.1016/j.jsb.2010.06.008
PMID: 20541610

A prominent β-hairpin structure in the winged-helix domain of RECQ1 is required for DNA unwinding and oligomer formation.
Lucic B, Zhang Y, King O, Mendoza-Maldonado R, Berti M, Niesen FH, Burgess-Brown NA, Pike AC, Cooper CD, Gileadi O, Vindigni A
Nucleic Acids Res.. 2010 39(5):1703-17. doi: 10.1093/nar/gkq1031
PMID: 21059676

Large-scale structural analysis of the classical human protein tyrosine phosphatome.
Barr AJ, Ugochukwu E, Lee WH, King ON, Filippakopoulos P, Alfano I, Savitsky P, Burgess-Brown NA, Müller S, Knapp S
Cell. 2009 136(2):352-63. doi: 10.1016/j.cell.2008.11.038
PMID: 19167335

Structure of the human RECQ1 helicase reveals a putative strand-separation pin.
Pike AC, Shrestha B, Popuri V, Burgess-Brown N, Muzzolini L, Costantini S, Vindigni A, Gileadi O
Proc. Natl. Acad. Sci. U.S.A.. 2009 106(4):1039-44. doi: 10.1073/pnas.0806908106
PMID: 19151156

High throughput production of recombinant human proteins for crystallography.
Gileadi O, Burgess-Brown NA, Colebrook SM, Berridge G, Savitsky P, Smee CE, Loppnau P, Johansson C, Salah E, Pantic NH
Methods Mol. Biol.. 2008 426:221-46. doi: 10.1007/978-1-60327-058-8_14
PMID: 18542867

Codon optimization can improve expression of human genes in Escherichia coli: A multi-gene study.
Burgess-Brown NA, Sharma S, Sobott F, Loenarz C, Oppermann U, Gileadi O
Protein Expr. Purif.. 2008 59(1):94-102. doi: 10.1016/j.pep.2008.01.008
PMID: 18289875

Proteomic analysis of the cell-surface membrane in chronic lymphocytic leukemia: identification of two novel proteins, BCNP1 and MIG2B.
Boyd, RS; Adam, PJ; Patel, S; Loader, JA; Berry, J; Redpath, NT; Poyser, HR; Fletcher, GC; Burgess, NA; Stamps, AC; Hudson, L; Smith, P; Griffiths, M; Willis, TG; Karran, EL; Oscier, DG; Catovsky, D; Terrett, JA; Dyer, MJS;
Leukemia. 2003 17:1605-1612. doi: 10.1038/sj.leu.2402993
PMID: 12886250

2020

A lower X-gate in TASK channels traps inhibitors within the vestibule
RODSTROM K, Kiper A, Zhang W, Rinne S, PIKE A, Goldstein M, Conrad L, Delbeck M, Hahn M, Meier H, Platzk M, Quigley A, Speedman D, SHRESTHA L, Mukhopadhyay S, BURGESS-BROWN N, TUCKER S, Mueller T, Decher N, CARPENTER E
Nature. 2020 582:443-447. doi: 10.1038/s41586-020-2250-8
PMID: 32499642

2019

The structural basis of lipid scrambling and inactivation in the endoplasmic reticulum scramblase TMEM16K
Bushell SR, Pike ACW, Falzone ME, Rorsman NJG, Ta CM, Corey RA, Newport TD, Christianson JC, Scofano LF, Shintre CA, Tessitore A, Chu A, Wang Q, Shrestha L, Mukhopadhyay SMM, Love JD, Burgess-Brown NA, Sitsapesan R, Stansfeld PJ, Huiskonen JT, Tammaro P, Accardi A, Carpenter EP
Nature Communications. 2019 10:3956-. doi: 10.1038/s41467-019-11753-1
PMID: 31477691

High-throughput site-directed mutagenesis
Strain-Damerell C, Burgess-Brown NA
Methods Mol Biol. 2019 2025:281-296. doi: 10.1007/978-1-4939-9624-7_13
PMID: 31267458

2018

Structures of DPAGT1 Explain Glycosylation Disease Mechanisms and Advance TB Antibiotic Design.
Dong YY, Wang H, Pike ACW, Cochrane SA, Hamedzadeh S, Wyszyński FJ, Bushell SR, Royer SF, Widdick DA, Sajid A, Boshoff HI, Park Y, Lucas R, Liu WM, Lee SS, Machida T, Minall L, Mehmood S, Belaya K, Liu WW, Chu A, Shrestha L, Mukhopadhyay SMM, Strain-Damerell C, Chalk R, Burgess-Brown NA, Bibb MJ, Barry Iii CE, Robinson CV, Beeson D, Davis BG, Carpenter EP
Cell. 2018 175(4):1045-1058.e16. doi: 10.1016/j.cell.2018.10.037
PMID: 30388443

Tryptophan-Mediated Interactions between Tristetraprolin and the CNOT9 Subunit Are Required for CCR4-NOT Deadenylase Complex Recruitment.
Bulbrook, D; Brazier, H; Mahajan, P; Kliszczak, M; Fedorov, O; Marchese, FP; Aubareda, A; Chalk, R; Picaud, S; Strain-Damerell, C; Filippakopoulos, P; Gileadi, O; Clark, AR; Yue, WW; Burgess-Brown, NA; Dean, JLE;
Journal of Molecular Biology. 2018 430:722-736. doi: 10.1016/j.jmb.2017.12.018
PMID: 29291391

2017

The SGC beyond structural genomics: redefining the role of 3D structures by coupling genomic stratification with fragment-based discovery.
Bradley AR, Echalier A, Fairhead M, Strain-Damerell C, Brennan P, Bullock AN, Burgess-Brown NA, Carpenter EP, Gileadi O, Marsden BD, Lee WH, Yue W, Bountra C, von Delft F
Essays Biochem.. 2017 61(5):495-503. doi: 10.1042/EBC20170051
PMID: 29118096

Potent and Selective KDM5 Inhibitor Stops Cellular Demethylation of H3K4me3 at Transcription Start Sites and Proliferation of MM1S Myeloma Cells.
Tumber A, Nuzzi A, Hookway ES, Hatch SB, Velupillai S, Johansson C, Kawamura A, Savitsky P, Yapp C, Szykowska A, Wu N, Bountra C, Strain-Damerell C, Burgess-Brown NA, Ruda GF, Fedorov O, Munro S, England KS, Nowak RP, Schofield CJ, La Thangue NB, Pawlyn C, Davies F, Morgan G, Athanasou N, Müller S, Oppermann U, Brennan PE
Cell Chem Biol. 2017 . doi: 10.1016/j.chembiol.2017.02.006
PMID: 28262558

2016

Structure of the polycystic kidney disease TRP channel Polycystin-2 (PC2).
Grieben M, Pike AC, Shintre CA, Venturi E, El-Ajouz S, Tessitore A, Shrestha L, Mukhopadhyay S, Mahajan P, Chalk R, Burgess-Brown NA, Sitsapesan R, Huiskonen JT, Carpenter EP
Nat. Struct. Mol. Biol.. 2016 . doi: 10.1038/nsmb.3343
PMID: 27991905

Crystal structure of Porphyromonas gingivalis peptidylarginine deiminase: implications for autoimmunity in rheumatoid arthritis
Montgomery, AB; Kopec, J; Shrestha, L; Thezenas, M-L; Burgess-Brown, NA; Fischer, R; Yue, WW; Venables, PJ;
Annals of the Rheumatic Diseases. 2016 75:1255-1261. doi: 10.1136/annrheumdis-2015-207656
PMID:

Structural analysis of human KDM5B guides histone demethylase inhibitor development
Johansson, C; Velupillai, S; Tumber, A; Szykowska, A; Hookway, ES; Nowak, RP; Strain-Damerell, C; Gileadi, C; Philpott, M; Burgess-Brown, N; Wu, N; Kopec, J; Nuzzi, A; Steuber, H; Egner, U; Badock, V; Munro, S; LaThangue, NB; Westaway, S; Brown, J; Athanasou, N; Prinjha, R; Brennan, PE; Oppermann, U;
Nature Chemical Biology. 2016 12:539-545. doi: 10.1038/nchembio.2087
PMID: 27214403

8-Substituted Pyrido[3,4-d]pyrimidin-4(3H)-one Derivatives As Potent, Cell Permeable, KDM4 (JMJD2) and KDM5 (JARID1) Histone Lysine Demethylase Inhibitors.
Bavetsias V, Lanigan RM, Ruda GF, Atrash B, McLaughlin MG, Tumber A, Mok NY, Le Bihan YV, Dempster S, Boxall KJ, Jeganathan F, Hatch SB, Savitsky P, Velupillai S, Krojer T, England KS, Sejberg J, Thai C, Donovan A, Pal A, Scozzafava G, Bennett JM, Kawamura A, Johansson C, Szykowska A, Gileadi C, Burgess-Brown NA, von Delft F, Oppermann U, Walters Z, Shipley J, Raynaud FI, Westaway SM, Prinjha RK, Fedorov O, Burke R, Schofield CJ, Westwood IM, Bountra C, Müller S, van Montfort RL, Brennan PE, Blagg J
J. Med. Chem.. 2016 . doi: 10.1021/acs.jmedchem.5b01635
PMID: 26741168

2015

Crystal structure of Porphyromonas gingivalis peptidylarginine deiminase: implications for autoimmunity in rheumatoid arthritis.
Montgomery AB, Kopec J, Shrestha L, Thezenas ML, Burgess-Brown NA, Fischer R, Yue WW, Venables PJ
Ann. Rheum. Dis.. 2015 . doi: 10.1136/annrheumdis-2015-207656
PMID: 26209657

K2P channel gating mechanisms revealed by structures of TREK-2 and a complex with Prozac.
Dong YY, Pike AC, Mackenzie A, McClenaghan C, Aryal P, Dong L, Quigley A, Grieben M, Goubin S, Mukhopadhyay S, Ruda GF, Clausen MV, Cao L, Brennan PE, Burgess-Brown NA, Sansom MS, Tucker SJ, Carpenter EP
Science. 2015 347(6227):1256-1259. doi: 10.1126/science.1261512
PMID: 25766236

2014

Optimisation of a triazolopyridine based histone demethylase inhibitor yields a potent and selective KDM2A (FBXL11) inhibitor.
England KS, Tumber A, Krojer T, Scozzafava G, Ng SS, Daniel M, Szykowska A, Che K, von Delft F, Burgess-Brown NA, Kawamura A, Schofield CJ, Brennan PE
Medchemcomm. 2014 5(12):1879-1886. doi: 10.1039/C4MD00291A
PMID: 26682034

Assessing cellular efficacy of bromodomain inhibitors using fluorescence recovery after photobleaching.
Philpott M, Rogers CM, Yapp C, Wells C, Lambert JP, Strain-Damerell C, Burgess-Brown NA, Gingras AC, Knapp S, Müller S
Epigenetics Chromatin. 2014 7:14. doi: 10.1186/1756-8935-7-14
PMID: 25097667

Machine-assisted synthesis of modulators of the histone reader BRD9 using flow methods of chemistry and frontal affinity chromatography
Guetzoyan, L; Ingham, RJ; Nikbin, N; Rossignol, J; Wolling, M; Baumert, M; Burgess-Brown, NA; Strain-Damerell, CM; Shrestha, L; Brennan, PE; Fedorov, O; Knapp, S; Ley, SV;
MedChemComm. 2014 5:540-546. doi: 10.1039/c4md00007b
PMID:

2013

Structural insights into functional overlapping and differentiation among myosin V motors
Nascimento, AFZ; Trindade, DM; Tonoli, CCC; De Giuseppe, PO; Assis, LHP; Honorato, RV; Deoliveira, PSL; Mahajan, P; Burgess-Brown, NA; Von Delft, F; Larson, RE; Murakami, MT;
Journal of Biological Chemistry. 2013 288:34131-34145. doi: 10.1074/jbc.M113.507202
PMID:

Medium-throughput production of recombinant human proteins: ligation-independent cloning.
Strain-Damerell C, Mahajan P, Gileadi O, Burgess-Brown NA
Methods Mol. Biol.. 2013 1091:55-72. doi: 10.1007/978-1-62703-691-7_4
PMID: 24203324

Medium-throughput production of recombinant human proteins: protein production in insect cells.
Mahajan P, Strain-Damerell C, Gileadi O, Burgess-Brown NA
Methods Mol. Biol.. 2013 1091:95-121. doi: 10.1007/978-1-62703-691-7_6
PMID: 24203326

The structural basis of ZMPSTE24-dependent laminopathies
Quigley, A; Dong, YY; Pike, ACW; Dong, L; Shrestha, L; Berridge, G; Stansfeld, PJ; Sansom, MSP; Edwards, AM; Bountra, C; Von Delft, F; Bullock, AN; Burgess-Brown, NA; Carpenter, EP;
Science. 2013 340:1604-1607. doi: 10.1126/science.1231513
PMID:

2012

The PHD and chromo domains regulate the ATPase activity of the human chromatin remodeler CHD4.
Watson AA, Mahajan P, Mertens HD, Deery MJ, Zhang W, Pham P, Du X, Bartke T, Zhang W, Edlich C, Berridge G, Chen Y, Burgess-Brown NA, Kouzarides T, Wiechens N, Owen-Hughes T, Svergun DI, Gileadi O, Laue ED
J. Mol. Biol.. 2012 422(1):3-17. doi: 10.1016/j.jmb.2012.04.031
PMID: 22575888

Plant growth regulator daminozide is a selective inhibitor of human KDM2/7 histone demethylases.
Rose NR, Woon EC, Tumber A, Walport LJ, Chowdhury R, Li XS, King ON, Lejeune C, Ng SS, Krojer T, Chan MC, Rydzik AM, Hopkinson RJ, Che KH, Daniel M, Strain-Damerell C, Gileadi C, Kochan G, Leung IK, Dunford J, Yeoh KK, Ratcliffe PJ, Burgess-Brown N, von Delft F, Muller S, Marsden B, Brennan PE, McDonough MA, Oppermann U, Klose RJ, Schofield CJ, Kawamura A
J. Med. Chem.. 2012 55(14):6639-43. doi: 10.1021/jm300677j
PMID: 22724510

2011

Human SNM1A and XPF-ERCC1 collaborate to initiate DNA interstrand cross-link repair.
Wang AT, Sengerová B, Cattell E, Inagawa T, Hartley JM, Kiakos K, Burgess-Brown NA, Swift LP, Enzlin JH, Schofield CJ, Gileadi O, Hartley JA, McHugh PJ
Genes Dev.. 2011 25(17):1859-70. doi: 10.1101/gad.15699211
PMID: 21896658

Human SNM1a and XPF-ERCC1 collaborate to initiate DNA interstrand cross-link repair
Wang, AT; Sengerová, B; Cattell, E; Inagawa, T; Hartley, JM; Kiakos, K; Burgess-Brown, NA; Swift, LP; Enzlin, JH; Schofield, CJ; Gileadi, O; Hartley, JA; McHugh, PJ;
Genes and Development. 2011 25:1859-1870. doi: 10.1101/gad.15699211
PMID:

High-performance liquid chromatography separation and intact mass analysis of detergent-solubilized integral membrane proteins.
Berridge G, Chalk R, D'Avanzo N, Dong L, Doyle D, Kim JI, Xia X, Burgess-Brown N, Deriso A, Carpenter EP, Gileadi O
Anal. Biochem.. 2011 410(2):272-80. doi: 10.1016/j.ab.2010.11.008
PMID: 21093405

2010

High-performance liquid chromatography separation and intact mass analysis of detergent-solubilized integral membrane proteins
Berridge, G; Chalk, R; Burgess-Brown, NA; Carpenter, EP; Gileadi, O;
Analytical Biochemistry. 2010 :-. doi: 10.1016/j.ab.2010.11.008
PMID:

A prominent β-hairpin structure in the winged-helix domain of RECQ1 is required for DNA unwinding and oligomer formation.
Lucic B, Zhang Y, King O, Mendoza-Maldonado R, Berti M, Niesen FH, Burgess-Brown NA, Pike AC, Cooper CD, Gileadi O, Vindigni A
Nucleic Acids Res.. 2010 39(5):1703-17. doi: 10.1093/nar/gkq1031
PMID: 21059676

High-throughput production of human proteins for crystallization: the SGC experience.
Savitsky P, Bray J, Cooper CD, Marsden BD, Mahajan P, Burgess-Brown NA, Gileadi O
J. Struct. Biol.. 2010 172(1):3-13. doi: 10.1016/j.jsb.2010.06.008
PMID: 20541610

2009

Structure of the human RECQ1 helicase reveals a putative strand-separation pin.
Pike AC, Shrestha B, Popuri V, Burgess-Brown N, Muzzolini L, Costantini S, Vindigni A, Gileadi O
Proc. Natl. Acad. Sci. U.S.A.. 2009 106(4):1039-44. doi: 10.1073/pnas.0806908106
PMID: 19151156

Large-scale structural analysis of the classical human protein tyrosine phosphatome.
Barr AJ, Ugochukwu E, Lee WH, King ON, Filippakopoulos P, Alfano I, Savitsky P, Burgess-Brown NA, Müller S, Knapp S
Cell. 2009 136(2):352-63. doi: 10.1016/j.cell.2008.11.038
PMID: 19167335

2008

High throughput production of recombinant human proteins for crystallography.
Gileadi O, Burgess-Brown NA, Colebrook SM, Berridge G, Savitsky P, Smee CE, Loppnau P, Johansson C, Salah E, Pantic NH
Methods Mol. Biol.. 2008 426:221-46. doi: 10.1007/978-1-60327-058-8_14
PMID: 18542867

Codon optimization can improve expression of human genes in Escherichia coli: A multi-gene study.
Burgess-Brown NA, Sharma S, Sobott F, Loenarz C, Oppermann U, Gileadi O
Protein Expr. Purif.. 2008 59(1):94-102. doi: 10.1016/j.pep.2008.01.008
PMID: 18289875

2005

The structural genomics consortium: High-output structure determination
Gileadi, O; Colebrook, S; Burgess, N; Berridge, G; Haroniti, A; Shrestha, B; Smee, C; Sundstrom, M;
FASEB Journal. 2005 19:A315-A315. doi:
PMID:

2003

Proteomic analysis of the cell-surface membrane in chronic lymphocytic leukemia: identification of two novel proteins, BCNP1 and MIG2B.
Boyd, RS; Adam, PJ; Patel, S; Loader, JA; Berry, J; Redpath, NT; Poyser, HR; Fletcher, GC; Burgess, NA; Stamps, AC; Hudson, L; Smith, P; Griffiths, M; Willis, TG; Karran, EL; Oscier, DG; Catovsky, D; Terrett, JA; Dyer, MJS;
Leukemia. 2003 17:1605-1612. doi: 10.1038/sj.leu.2402993
PMID: 12886250

2002

LuxS: its role in central metabolism and the in vitro synthesis of 4-hydroxy-5-methyl-3(2H)-furanone.
Winzer, K; Hardie, KR; Burgess, N; Doherty, N; Kirke, D; Holden, MTG; Linforth, R; Cornell, KA; Taylor, AJ; Hill, PJ; Williams, P;
Microbiology. 2002 148:909-922. doi: 10.1099/00221287-148-4-909
PMID: 11932438

LuxS-dependent quorum sensing in Porphyromonas gingivalis modulates protease and haemagglutinin activities but is not essential for virulence.
Burgess, NA; Kirke, DF; Williams, P; Winzer, K; Hardie, KR; Meyers, NL; Aduse-Opoku, J; Curtis, MA; Cámara, M;
Microbiology. 2002 148:763-772. doi: 10.1099/00221287-148-3-763
PMID: 11882711

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