Expression Vectors

The table below contains the entire collection of SGC’s expression vectors. To browse the collection, find associated data, or acquire samples, select the desired expression vector in the table below and read the PDF information sheet for the vector. You can sort the table by clicking the column headers. To purchase a vector, follow the links to AddGene and Source BioScience in the Description column.

Clone Vector ID GenBank Deposition ID SGC Site Description
p11 Oxford/Toronto

E. coli expression vector with N-terminal Hexa-HIS and TEV cleavage

p15 Oxford

E. coli expression vector with C-terminal His6 tag and TEV cleavage

p15TV-L EF456736 Toronto

E. coli expression vector with N-terminal Hexa-HIS and TEV cleavage available from AddGene

p21a-LIC EF456737 Toronto

E. coli expression vector with N-terminal Hexa-HIS and TEV cleavage

p28BIOH-LIC KC164371 Toronto

E. coli expression vector with N-terminal AviTag and a C-terminal Hexa-HIS tag

p28BIOHTEV-LIC Toronto

E. coli expression vector with N-terminal TEV cleavable AviTag and a C-terminal Hexa-HIS tag

pAB-LIC Toronto

Baculovirus expression vector for secretion with N-terminal Honeybee melittin signal and C-terminal 8xHis tag

pACYC-GST Toronto

E. coli pACYC based expression vector with N-terminal TEV cleavable GST-tag

pACYC-LIC+ Oxford/Toronto

E. coli pACYC based expression vector with N-terminal Hexa-HIS and a thrombin protease cleavage site

pBacMam2-SDiEx-LIC Toronto

Baculovirus and mammalian expression vector with N-terminal signal and C-terminal fusion His10 and FLAG tag preceded by a TEV protease cleavage site

pBEN1-SGC GU725055 Oxford

E. coli expression vector with N-terminal Strep tag and SET1 (solubility enhancement tag) available from Source BioScience Life Sciences

pCD33-CH Toronto

Mammalian expression vector with N-terminal CD33 signal peptide and C-terminal Hexa-HIS

pCDF-BirA JF914075 Oxford

E. coli co-expression vector containing the biotin-protein ligase gene

pCDF-Duet-1-LIC1 Toronto

E. coli co-expression vector derived from pCDF-Duet-1with N-terminal His tag followed by TEV cleavage site preceded by 1st promoter. This vector is compatible with pET and pACYC vectors

pCDF-LIC Oxford

E. coli co-expression vector with N-terminal His6 tag and TEV cleavage, compatible with pET and pACYC vectors

pcDNA3-C-Flag-LIC Oxford

Mammalian expression vector with C-terminal Flag tag

pcDNA3-C-GFP-LIC Oxford

Mammalian expression vector with C-terminal GFP tag

pcDNA3-CT10HF-LIC Oxford

Mammalian expression vector with C-terminal His10-Flag tags and TEV cleavage

pcDNA3-N-Flag-LIC Oxford

Mammalian expression vector with N-terminal Flag tag

pcDNA3-N-GFP-LIC Oxford

Mammalian expression vector with N-terminal GFP tag

pCOEX-1 Oxford

E. coli co-expression vector with N-terminal His7 tag and TEV cleavage, pACYC backbone

pCW-LIC EF460848 Toronto

E. coli expression vector available from AddGene

pER-NH Toronto

Mammalian based expression vector with N-terminal ER signal peptide and Hexa-HIS

pET-6HisMBP-LIC Toronto

E. coli expression vector with N-terminal 6 x His and MBP fusion tags

pET15-MHL EF456738 Toronto

E. coli expression vector with N-terminal Hexa-HIS and TEV cleavage available from AddGene

pET28-CFP-YFP Toronto

E. coli expression vector with N-terminal Hexa-HIS, CFP and TEV cleavage, and C-terminal YFP

pET28-CUb Toronto

E. coli expression vector with N-terminal Hexa-HIS and TEV cleavage and coexpression of ubiquitin at C-terminal.

pET28-FLAG Toronto

E. coli expression vector with N-terminal Hexa-HIS, FLAG tag, and TEV cleavage

pET28-MHL EF456735 Toronto

E. coli expression vector with N-terminal Hexa-HIS and TEV cleavage available from AddGene

pET28-MKH8SUMO Toronto

E. coli expression vector with N-terminal Hexa-HIS, thrombin,SUMO and TEV cleavage

pET28-SBP Toronto

E. coli expression vector with N-terminal Hexa-HIS and SBP tag

pET28-SBP-TEV Toronto

E. coli expression vector with N-terminal Hexa-HIS, SBP tag and TEV cleavage available from AddGene

pET28a-LIC EF442785 Toronto

E. coli expression vector with N-terminal Hexa-HIS and Thrombin cleavage available from AddGene

pET28GST-LIC EF456739 Toronto

E. coli expression vector with N-terminal Hexa-HIS, GSTtag, and Thrombin cleavage available from AddGene

pET28GSTlinker-LIC Toronto

E. coli expression vector with a long linker between N-terminal GST tag and TEV cleavage site. The linker is designed to leave GST-tag free in DNA binding screening experiments

pET32a-LIC EF442786 Toronto

E. coli expression vector with N-terminal Trx-tag, 6X His-tag, thrombin site, S-tag, and TEV

pETDuet-1-LIC1 Toronto

E. coli co-expression vector derived from pET-Duet-1with N-terminal His tag followed by TEV cleavage site preceded by 1st promoter. This vector is compatible with pACYC vector.

pFB-6HisMBP-TEV-MHL Toronto

Baculovirus expression vector with N-terminal 6 x His and MBP fusion tags

pFB-6HZB Oxford

Baculovirus expression vector with N-terminal His6-ZB tags and TEV cleavage

pFB-CFlag-LIC Toronto

Baculovirus expression vector with C-terminal Flag Tag

pFB-CHis-LIC Toronto

Baculovirus expression vector with C-terminal Hexa-His Tag

pFB-CT10HF-LIC Oxford

Baculovirus expression vector with C-terminal His10-Flag tags and TEV cleavage available from AddGene and Source BioScience Life Sciences

pFB-CTevHis-LIC Toronto

Baculovirus expression vector with C-terminal TEV cleavage and Hexa-His Tag

pFB-HGT-LIC Oxford

Baculovirus expression vector with N-terminal His6-GST tags and TEV cleavage

pFB-LIC-Bse EF199842 Oxford

Baculovirus expression vector with N-terminal His6 tag and TEV cleavage available from AddGene and Source BioScience Life Sciences

pFB-Mam-CT10HF-LIC Oxford

BacMam expression vector C-terminal His10-Flag tags and TEV cleavage

pFB-Nflag-LIC Toronto

Baculovirus expression vector with N-terminal Flag tag followed by TEV cleavage site

pFB-nHB-cTEVhisAvi-LIC Toronto

Baculovirus expression vector for secretion with C-terminal fusion tags of TEV- His-Avi

pFB-NHT-CF-Bse Oxford

Baculovirus expression vector with N-terminal His6, TEV cleavage and C-terminal Flag tag

pFB-Sec-NH Oxford

Baculovirus secretion vector with gp64 signal peptide, N-terminal His6 tag and TEV cleavage available from AddGene and Source BioScience Life Sciences

pFB-ZB JF682518 Oxford

Baculovirus expression vector with N-terminal His10-ZB tags and TEV cleavage

pFBD-BirA Toronto

Baculovirus expression vector with N-terminal AviTag, C-terminal Hexa-HIS and Coexpression of BirA

pFBD-LIC1 Toronto

Baculovirus dual expression vector with N-terminal His tag followed by a thrombin cleavage site preceded by Polyhedrin promoter. The P10 promoter is untagged, but the Nco I site under P10 is destroyed which gives more flexibility of removing or replacing His-tag by Nco I and BseR I cut.

pFBIP-C Toronto

Baculovirus expression vector with N-terminal BiP signal peptide, Hexa-His, and TEV cleavage site

pFBIP-N Toronto

Baculovirus expression vector with N-terminal BiP signal peptide and C-terminal Hexa-His

pFBOH-avi-TEV-LIC Toronto

Baculovirus expression vector with N-terminal Hexa-His, AviTag, and TEV cleavage

pFBOH-GST Toronto

Baculovirus expression vector with N-terminal GST tag, Hexa-HIS and TEV cleavage

pFBOH-LIC EF456740 Toronto

Baculovirus expression vector with N-terminal Hexa-HIS and Thrombin cleavage available from AddGene

pFBOH-MHL Toronto

Baculovirus expression vector with N-terminal Hexa-HIS and TEV cleavage

pFBOH-SBP-TEV-LIC Toronto

Baculovirus expression vector with N-terminal Hexa-HIS, SBP-tag, and TEV cleavage

pFBOH-SUMOstar Toronto

Baculovirus expression vector with N-terminal Hexa-HIS and SUMOstar fusion protein

pFBOH-SUMOstar-TEV Toronto

Baculovirus expression vector with N-terminal Hexa-HIS, SUMOstar fusion protein, and TEV cleavage

pFGP64-C Toronto

Baculovirus expression vector for secretion with N-terminal Autographa californica nuclear polyhedrosis virus gp64 signal peptide and C-terminal Hexa-His.

pFGP64-N Toronto

Baculovirus expression vector for secretionwith N-terminal Autographa californica nuclear polyhedrosis virus gp64 signal peptide, Hexa-His, and TEV cleavage

pFHMSP-LIC-C Toronto

Baculovirus expression vector for secretion with C-terminal Hexa-HIS available from AddGene

pFHMSP-LIC-N Toronto

Baculovirus expression vector for secretion with N-terminal Hexa-HIS and TEV cleavage available from AddGene

pFHMSPN-avi-TEV-LIC Toronto

Baculovirus expression vector for secretion with N-terminal Hexa-HIS, Avi-tag and TEV cleavage

pFHMSPN-SBP-TEV-LIC Toronto

Baculovirus expression vector for secretion with N-terminal Hexa-HIS, SBP-tag and TEV cleavage

pFSTREP-C Toronto

Baculovirus expression vector for secretion with N-terminal Apis mellifera melittin signal peptide and C-terminal Strep-tag

pFSTREP-N Toronto

Baculovirus expression vector for secretion with N-terminal Apis mellifera melittin signal peptide, Strep-tag, and TEV cleavage

pFTRYP-C Toronto

Baculovirus expression vector for secretion with N-terminal Homo sapiens trypsin 2 signal peptide and a C-terminal Hexa-His

pFTRYP-EK-C Toronto

Baculovirus expression vector for secretion with N-terminal Homo sapiens trypsin 2 signal peptide, enterokinase cleavage site and C-terminal Hepta-His

pFTRYP-EK-N Toronto

Baculovirus expression vector  for secretion with N-terminal Homo sapiens trypsin 2 signal peptide, enterokinase cleavage site , Hexa-His, and TEV cleavage

pFTRYP-N Toronto

Baculovirus expression vector  for secretion with N-terminal Homo sapiens trypsin 2 signal peptide, Hexa-His, and TEV cleavage

pFUSE-HEAVY KC176268 Toronto

Mammalian expression vector for IgG HC production

pFUSE-LIGHT KC176267 Toronto

Mammalian expression vector for IgG LC production

pGTvL1-SGC Oxford

E. coli expression vector with GST and TEV cleavage available from AddGene and Source BioScience Life Sciences

pGTVL2 JF522100 Oxford

E. coli expression vector with His6-GST and TEV cleavage 
 

pLIC-SGC1 Oxford

E. coli expression vector with His6 and TEV cleavage available from AddGene and Source BioScience Life Sciences
 

pNH-TrxT GU269914 Oxford

E. coli expression vector with N-terminal His6-Thioredoxin and TEV cleavage available from AddGene and Source BioScience Life Sciences
 

pNIC-Bio1 Oxford

E. coli expression vector with N-terminal His10, TEV cleavage and C-terminal Avi tag
 

pNIC-Bio2 JF912191 Oxford

E. coli expression vector with N-terminal His10, TEV cleavage and C-terminal Avi tag

pNIC-Bio3 JN792439 Oxford

E. coli expression vector with N-terminal His6, TEV cleavage and C-terminal Avi tag

pNIC-CH EF199843 Oxford

E. coli expression vector with C-terminal His6 tag available from AddGene and Source BioScience Life Sciences

pNIC-CTHF EF199844 Oxford

E. coli expression vector with C-terminal His6-Flag tags and TEV cleavage available from AddGene and Source BioScience Life Sciences

pNIC-CTHO Oxford

E. coli expression vector with C-terminal His6 tag and TEV cleavage
 

pNIC-H102 JF912192 Oxford

E. coli expression vector with N-terminal His10 tag and TEV cleavage

pNIC-NHT-CF EF199845 Oxford

E. coli expression vector with N-terminal His6, TEV cleavage and C-terminal Flag tag
 

pNIC-ZB GU452710 Oxford

E. coli expression vector with N-terminal His6-ZB tags and TEV cleavage available from AddGene and Source BioScience Life Sciences

pNIC28-Bsa4 EF198106 Oxford

E. coli expression vector with N-terminal His6 tag and TEV cleavage available from AddGene and Source BioScience Life Sciences

pSFV4 KC164372 Toronto

E. coli expression vector for FABs with AviTag
 

pTRC-LIC EF460847 Toronto

E. coli expression vector

pTvHR21-SGC Oxford

E. coli expression vector with C-terminal His6 tag (for recombinase cloning) available from AddGene and Source BioScience Life Sciences