SGC-UBD1031 A probe with dual USP16-HDAC6 activity.

The probe and control may be requested here.

For any inquiries please contact proberequests@thesgc.org.

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Overview

SGC in collaboration with Dr Mark Lautens’ at the University of Toronto’s Department of Chemistry has developed the first chemical probe SGC-UBD1031 for the ubiquitin binding domain (UBD) of USP16. SGC-UBD1031 has equipotent affinity to HDAC6 UBD and it is recommended to be used in parallel with SGC-UBD253.

SGC-UBD1031 binds potently USP16 UBD with KD = 16 nM (SPR), and in cells it inhibits the USP16-ISG15 interaction with EC₅₀ = 1.5 micromolar (nanoBRET).

SGC-UBD1031N is a closely related negative control with no activity in the nanoBRET assay up to 30 micromolar.

Selectivity Profile

In Vitro Potency

Cell-based Assay Data